Plant Protein Secretion : Methods and Protocols

Front Matter ....Pages i-xi
 An Overview of Protein Secretion in Yeast and Animal Cells  (Yusong Guo, Feng Yang, Xiao Tang)....Pages 1-17
 An Overview of Protein Secretion in Plant Cells  (Kin Pan Chung, Yonglun Zeng)....Pages 19-32
 Bioinformatics Analysis of Protein Secretion in Plants  (Liyuan Chen)....Pages 33-43
 Proteomic Analysis of Secreted Proteins from Cell Microenvironment  (Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian)....Pages 45-58
 Using Homology Modeling to Understand the Structural Basis of Specific Interaction of a Plant-Specific AtSar1a–AtSec23a Pair Involved in Protein ER Export  (Yap-Shing Nim, Shuangli Sun, Kam-Bo Wong)....Pages 59-73
 Analysis of Golgi-Mediated Protein Traffic in Plant Cells  (Wenjin Shen, Zhidan Xiao, Jinbo Shen, Caiji Gao)....Pages 75-86
 Analysis of Membrane Protein Topology in the Plant Secretory Pathway  (Jinya Guo, Yansong Miao, Yi Cai)....Pages 87-95
 Semiautomatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod  (Keith Ka Ki Mai, Byung-Ho Kang)....Pages 97-104
 3D Printing of Plant Golgi Stacks from Their Electron Tomographic Models  (Keith Ka Ki Mai, Madison J. Kang, Byung-Ho Kang)....Pages 105-113
 Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics  (Guitao Zhong, Ronghe Liu, Menglong Zhuang, Hao Wang)....Pages 115-124
 Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes  (Yuxiang Jiang, Meng Zhang, Shanjin Huang)....Pages 125-136
 Analysis of Phragmoplast Kinetics During Plant Cytokinesis  (Pantelis Livanos, Mayank Chugh, Sabine Müller)....Pages 137-150
 Immunofluorescence Analysis of Membrane-Associated Proteins for Clathrin-Mediated Endocytosis in Plant Root Cells  (Chao Wang, Xu Yan, Tingting Meng, Tianwei Hu, Jianwei Pan)....Pages 151-157
 In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer Through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM)  (Florian Fäßler, Peter Pimpl)....Pages 159-170
 Analysis of Nanobody–Epitope Interactions in Living Cells via Quantitative Protein Transport Assays  (Simone Früholz, Peter Pimpl)....Pages 171-182
 A Secretion System for Cargo Protein Identification of Vacuolar Sorting Receptors  (Jinbo Shen)....Pages 183-191
 Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination  (Qiang-Nan Feng, Yan Zhang)....Pages 193-198
 Measuring Plant Protein Secretion  (Emily R. Larson)....Pages 199-207
 Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion  (Di Sansebastiano Gian Pietro, Barozzi Fabrizio)....Pages 209-221
 The Organelle pH Estimate and Measurement in Plant Secretory Pathway  (Jinbo Shen)....Pages 223-230
 Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion (UPS) in Plant Cells  (Yu Ding, Juan Wang)....Pages 231-241
 Isolation of the Plant Exocyst Complex  (King Pong Leung, Wilson Chun Yu Lau)....Pages 243-255
 Using Microscopy Tools to Visualize Autophagosomal Structures in Plant Cells  (Weili Lin, Xiaohong Zhuang)....Pages 257-266
 Analysis of Plant Autophagy  (Liang Chen, Faqiang Li, Shi Xiao)....Pages 267-280
Back Matter ....Pages 281-285