Plant Membrane Proteomics: Methods and Protocols

Front Matter ....Pages i-xii
 Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments Prior to Proteomic Analysis  (Bronwyn J. Barkla)....Pages 1-12
 Isolation and Purity Assessment of Membranes from Norway Spruce  (Enni Väisänen, Junko Takahashi, Luis A. Jiménez Barboza, Xianbao Deng, Teemu H. Teeri, Kurt V. Fagerstedt et al.)....Pages 13-39
 Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins, and 2-DE Analysis  (Aarti Pandey, Subhra Chakraborty, Niranjan Chakraborty)....Pages 41-55
 Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis  (Ivo Chamrád, Jana Uřinovská, Beáta Petrovská, Hana Jeřábková, René Lenobel, Jan Vrána et al.)....Pages 57-79
 Isolation, Purity Assessment, and Proteomic Analysis of Nuclei  (Setsuko Komatsu)....Pages 81-90
 Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient  (Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko et al.)....Pages 91-105
 Analyzing the Vacuolar Membrane (Tonoplast) Proteome  (Miwa Ohnishi, Katsuhisa Yoshida, Tetsuro Mimura)....Pages 107-116
 Preparation of Membrane Fractions (Envelope, Thylakoids, Grana, and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies  (Lucas Moyet, Daniel Salvi, Martino Tomizioli, Daphné Seigneurin-Berny, Norbert Rolland)....Pages 117-136
 Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria  (Ann Magnuson, Tanai Cardona)....Pages 137-145
 Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development  (Roque Bru-Martínez, Ascensión Martínez-Márquez, Jaime Morante-Carriel, Susana Sellés-Marchart, María José Martínez-Esteso, José Luis Pineda-Lucas et al.)....Pages 147-162
 Sample Preparation for Analysis of the Plant Mitochondrial Membrane Proteome  (Christine Schikowsky, Beate Thal, Hans-Peter Braun, Holger Eubel)....Pages 163-183
 Plasma Membrane Proteomics of Arabidopsis Suspension-Cultured Cells Associated with Growth Phase Using Nano-LC-MS/MS  (Bin Li, Daisuke Takahashi, Yukio Kawamura, Matsuo Uemura)....Pages 185-194
 Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS Analysis  (Anna M. Jozefowicz, Andrea Matros, Katja Witzel, Hans-Peter Mock)....Pages 195-204
 Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic Stresses  (Małgorzata Janicka, Anna Wdowikowska, Grażyna Kłobus)....Pages 205-215
 Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters  (Carsten Rautengarten, Berit Ebert, Joshua L. Heazlewood)....Pages 217-234
 MSE for Label-Free Absolute Protein Quantification in Complex Proteomes  (Stefan Helm, Sacha Baginsky)....Pages 235-247
 Identification and Characterization of Plant Membrane Proteins Using ARAMEMNON  (Rainer Schwacke, Ulf-Ingo Flügge)....Pages 249-259
 VANTED: A Tool for Integrative Visualization and Analysis of -Omics Data  (Anja Hartmann, Anna Maria Jozefowicz)....Pages 261-278
Back Matter ....Pages 279-280