Plant germline development : methods and protocols

Front Matter ....Pages i-xiii
Front Matter ....Pages 1-1
 An Introduction to Male Germline Development  (Hannes Vogler, Ueli Grossniklaus, Afif Hedhly)....Pages 3-15
 Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants  (Joann A. Conner, Peggy Ozias-Akins)....Pages 17-34
Front Matter ....Pages 35-35
 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice  (Ethel Mendocilla-Sato, Wenjing She, Célia Baroux)....Pages 37-45
 Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana  (Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, Tomokazu Kawashima)....Pages 47-54
 Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers  (Mark A. Chamberlin, Shai J. Lawit)....Pages 55-65
 Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy  (David Twell, Lynette Brownfield)....Pages 67-75
 In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes  (Burcu Nur Keçeli, Nico De Storme, Danny Geelen)....Pages 77-85
 Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins  (Daniel Slane, Patrick Bürgel, Martin Bayer)....Pages 87-94
 Live-Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes  (Antony M. Chettoor, Matthew M. S. Evans)....Pages 95-101
 Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy  (Christina Maria Franck, Jens Westermann, Aurélien Boisson-Dernier)....Pages 103-116
 Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures  (Mário Luís da Costa, Ana Lúcia Lopes, Maria Isabel Amorim, Sílvia Coimbra)....Pages 117-137
Front Matter ....Pages 139-139
 Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues  (Laksana Kantama, Erik Wijnker, Hans de Jong)....Pages 141-158
 Whole Mount RNA-FISH on Ovules and Developing Seeds  (Andrea Bleckmann, Thomas Dresselhaus)....Pages 159-171
 Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy  (Peng-Fei Jia, Hong-Ju Li, Wei-Cai Yang)....Pages 173-180
 Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes  (Peng-Fei Jia, Hong-Ju Li, Wei-Cai Yang)....Pages 181-189
Front Matter ....Pages 191-191
 Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS)  (Mário R. Santos, Cláudia Bispo, Jörg D. Becker)....Pages 193-210
 Isolation of Rice Sperm Cells for Transcriptional Profiling  (Scott D. Russell, Daniel S. Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, Xiaoping Gou)....Pages 211-219
 Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation  (Maria Englhart, Lucija Šoljić, Stefanie Sprunck)....Pages 221-234
Front Matter ....Pages 235-235
 Isolation and Detection of Small RNAs from Pollen  (German Martinez)....Pages 237-250
 Analysis of Proteins Enriched in Rice Gamete  (Takashi Okamoto)....Pages 251-263
 Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract  (Jan Fíla, David Honys)....Pages 265-274
 Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants  (Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, Lynette Brownfield)....Pages 275-293
 RNA-Seq Data Analysis: From Raw Data Quality Control to Differential Expression Analysis  (Weihong Qi, Ralph Schlapbach, Hubert Rehrauer)....Pages 295-307
 RNA-Seq Data Analysis Protocol: Combining In-House and Publicly Available Data  (Marc W. Schmid)....Pages 309-335
Front Matter ....Pages 337-337
 Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality  (Iris Heidmann, Marco Di Berardino)....Pages 339-354
 Agrobacterium-Mediated Sorghum Transformation  (Emily Wu, Zuo-Yu Zhao)....Pages 355-364
 Use of the Cas9 Orthologs from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana  (Jeannette Steinert, Carla Schmidt, Holger Puchta)....Pages 365-376
 Transgenic Reproductive Cell Ablation  (Shai J. Lawit, Mark A. Chamberlin)....Pages 377-386
 In Vivo Reporters for Protein Half-Life  (Pavel Reichman, Nico Dissmeyer)....Pages 387-406
 Conditional Modulation of Biological Processes by Low-Temperature Degrons  (Nico Dissmeyer)....Pages 407-416
Back Matter ....Pages 417-422