Plant Genome Editing with CRISPR Systems: Methods and Protocols

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
 Plant DNA Repair Pathways and Their Applications in Genome Engineering  (Qiudeng Que, Zhongying Chen, Tim Kelliher, David Skibbe, Shujie Dong, Mary-Dell Chilton)....Pages 3-24
Front Matter ....Pages 25-25
 Rapid Screening of CRISPR/Cas9-Induced Mutants Using the ACT-PCR Method  (Chun Wang, Kejian Wang)....Pages 27-32
 Decoding Sanger Sequencing Chromatograms from CRISPR-Induced Mutations  (Xianrong Xie, Xingliang Ma, Yao-Guang Liu)....Pages 33-43
Front Matter ....Pages 45-45
 Creating Large Chromosomal Deletions in Rice Using CRISPR/Cas9  (Riqing Li, Si Nian Char, Bing Yang)....Pages 47-61
 A Multiplexed CRISPR/Cas9 Editing System Based on the Endogenous tRNA Processing  (Kabin Xie, Yinong Yang)....Pages 63-73
 A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants  (Xu Tang, Zhaohui Zhong, Qiurong Ren, Binglin Liu, Yong Zhang)....Pages 75-82
 CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation  (Aimee Malzahn, Yong Zhang, Yiping Qi)....Pages 83-93
Front Matter ....Pages 95-95
 Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR/Cas9  (Weihang Gu, Dabing Zhang, Yiping Qi, Zheng Yuan)....Pages 97-107
 Knocking Out MicroRNA Genes in Rice with CRISPR-Cas9  (Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Qian Li, Xuelian Zheng, Yiping Qi et al.)....Pages 109-119
 An Agrobacterium-Mediated CRISPR/Cas9 Platform for Genome Editing in Maize  (Keunsub Lee, Huilan Zhu, Bing Yang, Kan Wang)....Pages 121-143
Front Matter ....Pages 145-145
 Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants  (Hanchuanzhi Yu, Yunde Zhao)....Pages 147-154
 Creating Targeted Gene Knockouts in Brassica oleracea Using CRISPR/Cas9  (Tom Lawrenson, Penny Hundleby, Wendy Harwood)....Pages 155-170
 Application of CRISPR/Cas9-Mediated Gene Editing in Tomato  (Nathan T. Reem, Joyce Van Eck)....Pages 171-182
 Genome Editing in Potato with CRISPR/Cas9  (Satya Swathi Nadakuduti, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, David S. Douches)....Pages 183-201
 Visual Assay for Gene Editing Using a CRISPR/Cas9 System in Carrot Cells  (Magdalena Klimek-Chodacka, Tomasz Oleszkiewicz, Rafal Baranski)....Pages 203-215
 Genome Editing in Soybean with CRISPR/Cas9  (Junqi Liu, Samatha Gunapati, Nicole T. Mihelich, Adrian O. Stec, Jean-Michel Michno, Robert M. Stupar)....Pages 217-234
 Genome Editing in Citrus Tree with CRISPR/Cas9  (Hongge Jia, Xiuping Zou, Vladimir Orbovic, Nian Wang)....Pages 235-241
Front Matter ....Pages 243-243
 Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems  (Yingxiao Zhang, Yong Zhang, Yiping Qi)....Pages 245-256
 Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1  (Xiaojia Yin, Abhishek Anand, Paul Quick, Anindya Bandyopadhyay)....Pages 257-268
 Targeted Mutagenesis Using FnCpf1 in Tobacco  (Akira Endo, Seiichi Toki)....Pages 269-281
Front Matter ....Pages 283-283
 Gene Replacement by Intron Targeting with CRISPR-Cas9  (Jun Li, Xiangbing Meng, Jiayang Li, Caixia Gao)....Pages 285-296
 Targeted Base Editing with CRISPR-Deaminase in Tomato  (Zenpei Shimatani, Tohru Ariizumi, Ushio Fujikura, Akihiko Kondo, Hiroshi Ezura, Keiji Nishida)....Pages 297-307
Front Matter ....Pages 309-309
 Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System  (Ahmed Mahas, Zahir Ali, Manal Tashkandi, Magdy M. Mahfouz)....Pages 311-326
 Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat  (Zhen Liang, Kunling Chen, Caixia Gao)....Pages 327-335
 DNA-Free Genome Editing via Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce  (Jongjin Park, Sunmee Choi, Slki Park, Jiyoung Yoon, Aiden Y. Park, Sunghwa Choe)....Pages 337-354
 An Automated Protoplast Transformation System  (Scott C. Lenaghan, C. Neal Stewart Jr.)....Pages 355-363
Back Matter ....Pages 365-367