Plant and Food Carotenoids: Methods and Protocols

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
 Pathways for Carotenoid Biosynthesis, Degradation, and Storage  (Tianhu Sun, Yaakov Tadmor, Li Li)....Pages 3-23
Front Matter ....Pages 25-25
 A Simple In Vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-Chain Prenyltransferases  (M. Victoria Barja, Manuel Rodríguez-Concepción)....Pages 27-38
 Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase In Vitro  (Maurizio Camagna, Ralf Welsch)....Pages 39-52
 Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO  (Eleanore T. Wurtzel, Jesús Beltrán)....Pages 53-61
 Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases  (Lourdes Gómez-Gómez, Gianfranco Diretto, Oussama Ahrazem, Salim Al-Babili)....Pages 63-74
 Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases  (Linda Dora Thomas, Srinivasagan Ramkumar, Johannes von Lintig)....Pages 75-88
 Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters  (Olivia Costantina Demurtas, Rita de Brito Francisco, Enrico Martinoia, Giovanni Giuliano)....Pages 89-99
Front Matter ....Pages 101-101
 Applications of Visible Spectroscopy and Color Measurements in the Assessments of Carotenoid Levels in Foods  (Paula Mapelli-Brahm, Francisco J. Rodríguez-Pulido, Carla M. Stinco, Francisco J. Heredia, Antonio J. Meléndez-Martínez)....Pages 103-116
 A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids  (Dámaso Hornero-Méndez)....Pages 117-134
 Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management  (Antonio Pérez-Gálvez, Isabel Viera, María Roca)....Pages 135-144
 cis/trans Carotenoid Extraction, Purification, Detection, Quantification, and Profiling in Plant Tissues  (Yagiz Alagoz, Namraj Dhami, Chris Mitchell, Christopher I. Cazzonelli)....Pages 145-163
 Determination of Plant Volatile Apocarotenoids  (José L. Rambla, Antonio Granell)....Pages 165-175
 A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants  (Jianing Mi, Kun-Peng Jia, Aparna Balakrishna, Salim Al-Babili)....Pages 177-188
 Fractionation of Tomato Fruit Chromoplasts  (Karel De Pourcq, Albert Boronat)....Pages 189-197
 Quantification of Strigolactones  (Carlos Rial, Rosa M. Varela, José M. G. Molinillo, Alexandra G. Durán, Francisco A. Macías)....Pages 199-208
 Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS  (Daniele Giuffrida, Mariosimone Zoccali, Luigi Mondello)....Pages 209-219
Front Matter ....Pages 221-221
 Elucidating Carotenoid Biosynthetic Enzyme Localization and Interactions Using Fluorescent Microscopy  (Maria Shumskaya, Rena F. Quinlan, Eleanore T. Wurtzel)....Pages 223-234
 Visualization of Carotenoid-Storage Structures in Fruits by Transmission Electron Microscopy  (Joanna Lado, Jaime Zacarias, María Jesús Rodrigo, Lorenzo Zacarías)....Pages 235-244
 Light Microscopy and Raman Imaging of Carotenoids in Plant Cells In Situ and in Released Carotene Crystals  (Tomasz Oleszkiewicz, Marta Z. Pacia, Ewa Grzebelus, Rafal Baranski)....Pages 245-260
Front Matter ....Pages 261-261
 Carotenoid Isotopolog Profiling in 13C-Labeled Leaf Extracts by LC-MS and LC-FTICR-MS  (Björn Thiele, Shizue Matsubara)....Pages 263-277
 Quantification of Carotenoid Pathway Flux in Green and Nongreen Systems  (Julian Koschmieder, Ralf Welsch)....Pages 279-291
 Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis  (Juan M. García, María J. Pozo, Juan A. López-Ráez)....Pages 293-299
Front Matter ....Pages 301-301
 Use of Potyvirus Vectors to Produce Carotenoids in Plants  (José-Antonio Daròs)....Pages 303-312
 Agrobacterium tumefaciens-Mediated Stable Transformation of Daucus carota  (Christian Gonzalez-Calquin, Claudia Stange)....Pages 313-320
 CRISPR-Cas-Mediated Gene Knockout in Tomato  (Gwen Swinnen, Thomas Jacobs, Laurens Pauwels, Alain Goossens)....Pages 321-341
 Modulation of Activity of a Carotenoid Pathway Through the Use of the TeT-on Regulatory System: Application in the Fungus Fusarium fujikuroi  (Julia Marente, Pedro Ortega, Javier Pardo-Medina, Javier Avalos, M. Carmen Limón)....Pages 343-360
Front Matter ....Pages 361-361
 Intervention Studies in Humans  (Begoña Olmedilla-Alonso)....Pages 363-373
 In Vitro Digestion for Control and Monitoring of Food Effects in Relation to Micellarization Index of Carotenoids  (Antonio Pérez-Gálvez, Javier Fontecha)....Pages 375-386
 Evaluation of Carotenoids Protection Against Oxidative Stress in the Animal Model Caenorhabditis elegans  (Patricia Martorell, Silvia Llopis, José Vicente Gil, Salvador Genovés, Daniel Ramón, Lorenzo Zacarías et al.)....Pages 387-401
 Mouse Models to Study Antiobesogenic Effects of Carotenoids  (Joan Ribot, Bojan Stojnic, Andreu Palou, M. Luisa Bonet)....Pages 403-417
Back Matter ....Pages 419-421