Phytoplasmas: Methods and Protocols

Front Matter ....Pages i-xi
 Phytoplasmas: An Introduction  (L. Pagliari, R. Musetti)....Pages 1-6
Front Matter ....Pages 7-7
 Micro-Tom Tomato Grafting for Stolbur-Phytoplasma Transmission: Different Grafting Techniques  (Sara Buoso, Alberto Loschi)....Pages 9-19
 Phytoplasma Transmission: Insect Rearing and Infection Protocols  (L. Pagliari, J. Chuche, D. Bosco, D. Thiéry)....Pages 21-36
 Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors  (Kerstin Krüger, Nicola Fiore)....Pages 37-52
 Symptoms of Phytoplasma Diseases  (Paolo Ermacora, Ruggero Osler)....Pages 53-67
Front Matter ....Pages 69-69
 Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas  (Carmine Marcone)....Pages 71-81
 Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene  (Assunta Bertaccini, Samanta Paltrinieri, Nicoletta Contaldo)....Pages 83-95
 PCR-Based Sequence Analysis on Multiple Genes Other than 16S rRNA Gene for Differentiation of Phytoplasmas  (Marta Martini, Kristi D. Bottner-Parker, Ing-Ming Lee)....Pages 97-115
 Real-Time PCR Protocol for Phytoplasma Detection and Quantification  (Yusuf Abou-Jawdah, Vicken Aknadibossian, Maan Jawhari, Patil Tawidian, Peter Abrahamian)....Pages 117-130
 Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in Its Host Plant without External Standard Curves  (Sanja Baric)....Pages 131-141
 A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas  (Shigeyuki Kakizawa)....Pages 143-149
 One-Step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas  (Ioanna Malandraki, Christina Varveri, Nikon Vassilakos)....Pages 151-157
 A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification  (Claudio Ratti, Stefano Minguzzi, Massimo Turina)....Pages 159-169
 Quantitative Analysis with Droplet Digital PCR  (Nataša Mehle, Tanja Dreo)....Pages 171-186
 Rapid Sample Preparation and LAMP for Phytoplasma Detection  (Jennifer Hodgetts)....Pages 187-201
 Assembly of Phytoplasma Genome Drafts from Illumina Reads Using Phytoassembly  (Cesare Polano, Giuseppe Firrao)....Pages 203-211
 Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of “Flavescence Dorée”  (H. Al-Saddik, A. Laybros, J. C. Simon, F. Cointault)....Pages 213-238
 Transcriptomic Analyses of Phytoplasmas  (Davide Pacifico, Simona Abbà, Sabrina Palmano)....Pages 239-251
Front Matter ....Pages 253-253
 Sieve Elements: The Favourite Habitat of Phytoplasmas  (Aart J. E. van Bel)....Pages 255-277
 Laser Microdissection of Phytoplasma-Infected Grapevine Leaf Phloem Tissue for Gene Expression Study  (Simonetta Santi)....Pages 279-290
 Collection of Phloem Sap in Phytoplasma-Infected Plants  (Matthias R. Zimmermann, Torsten Knauer, Alexandra C. U. Furch)....Pages 291-299
 DAPI and Confocal Laser-Scanning Microscopy for In Vivo Imaging of Phytoplasmas  (Rita Musetti, Stefanie Vera Buxa)....Pages 301-306
 Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas  (Luciana Galetto, Marta Vallino, Mahnaz Rashidi, Cristina Marzachì)....Pages 307-317
Front Matter ....Pages 319-319
 Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets Using Bimolecular Fluorescence Complementation (BiFC)  (Katrin Janik, Hagen Stellmach, Cecilia Mittelberger, Bettina Hause)....Pages 321-331
 Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants  (Jürgen Gross, Jannicke Gallinger, Margit Rid)....Pages 333-343
 Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-Infected Plants  (Marilia Almeida-Trapp, Axel Mithöfer)....Pages 345-358
Back Matter ....Pages 359-362