Phage Display: Methods and Protocols

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
 Construction of Human Immune and Naive scFv Libraries  (Jonas Kügler, Florian Tomszak, André Frenzel, Michael Hust)....Pages 3-24
 Construction of Naive and Immune Human Fab Phage-Display Library  (Noorsharmimi Omar, Theam Soon Lim)....Pages 25-44
 Construction of Synthetic Antibody Phage-Display Libraries  (Johan Nilvebrant, Sachdev S. Sidhu)....Pages 45-60
 Modular Construction of Large Non-Immune Human Antibody Phage-Display Libraries from Variable Heavy and Light Chain Gene Cassettes  (Nam-Kyung Lee, Scott Bidlingmaier, Yang Su, Bin Liu)....Pages 61-82
 Construction of Macaque Immune-Libraries  (Arnaud Avril, Sebastian Miethe, Michael Hust, Thibaut Pelat)....Pages 83-112
 Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format  (Ulrike S. Diesterbeck)....Pages 113-131
 Construction of Rabbit Immune Antibody Libraries  (Thi Thu Ha Nguyen, Jong Seo Lee, Hyunbo Shim)....Pages 133-146
 Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries  (Julius Grzeschik, Doreen Könning, Steffen C. Hinz, Simon Krah, Christian Schröter, Martin Empting et al.)....Pages 147-167
 Construction of High-Quality Camel Immune Antibody Libraries  (Ema Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, Baptiste Monsion)....Pages 169-187
 Construction of Chicken Antibody Libraries  (Jeanni Fehrsen, Susan Wemmer, Wouter van Wyngaardt)....Pages 189-203
 Construction and Selection of Affilin® Phage Display Libraries  (Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, Eva Bosse-Doenecke)....Pages 205-238
 Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies  (Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau)....Pages 239-253
 Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach  (Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, Nai-Kong V. Cheung)....Pages 255-269
Front Matter ....Pages 271-271
 Parallelized Antibody Selection in Microtiter Plates  (Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, André Frenzel et al.)....Pages 273-284
 Mass Spectrometry Immuno Assay (MSIA™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning  (Chai Fung Chin, Yee Siew Choong, Theam Soon Lim)....Pages 285-299
 Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection  (Angela Chiew Wen Ch’ng, Nurul Hamizah Binti Hamidon, Zoltán Konthur, Theam Soon Lim)....Pages 301-319
 Phage Display and Selections on Cells  (Wieland Fahr, André Frenzel)....Pages 321-330
 Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens  (Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, Bin Liu)....Pages 331-347
 Antibody Isolation From a Human Synthetic Combinatorial and Other Libraries of Single-Chain Antibodies  (Almog Bitton, Limor Nahary, Itai Benhar)....Pages 349-363
 Screening Phage-Display Antibody Libraries Using Protein Arrays  (Ricardo Jara-Acevedo, Paula Díez, María González-González, Rosa María Dégano, Nieves Ibarrola, Rafael Góngora et al.)....Pages 365-380
 Antibody Selection on FFPE Tissue Slides  (Andre ten Haaf, Stefan Gattenlöhner, Mehmet Kemal Tur)....Pages 381-391
 Antibody Affinity and Stability Maturation by Error-Prone PCR  (Tobias Unkauf, Michael Hust, André Frenzel)....Pages 393-407
Front Matter ....Pages 409-409
 Upgrading Affinity Screening Experiments by Analysis of Next-Generation Sequencing Data  (Christian Grohmann, Michael Blank)....Pages 411-424
 Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Discovery  (Kevin A. Henry)....Pages 425-446
 High-Throughput IgG Reformatting and Expression  (Chao-Guang Chen, Georgina Sansome, Michael J. Wilson, Con Panousis)....Pages 447-461
 Monitoring Phage Biopanning by Next-Generation Sequencing  (Anna Vaisman-Mentesh, Yariv Wine)....Pages 463-473
Front Matter ....Pages 475-475
 ORFeome Phage Display  (Jonas Zantow, Gustavo Marçal Schmidt Garcia Moreira, Stefan Dübel, Michael Hust)....Pages 477-495
 Epitope Mapping by Phage Display  (Gustavo Marçal Schmidt Garcia Moreira, Viola Fühner, Michael Hust)....Pages 497-518
 Metasecretome Phage Display  (Milica Ciric, Filomena Ng, Jasna Rakonjac, Dragana Gagic)....Pages 519-534
 Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages  (Gertrudis Rojas, Tania Carmenate)....Pages 535-560
Back Matter ....Pages 561-562