Peptide Self-Assembly

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
 Methods to Characterize the Nanostructure and Molecular Organization of Amphiphilic Peptide Assemblies  (V. Castelletto, I. W. Hamley)....Pages 3-21
 Solid-State NMR Structural Characterization of Self-Assembled Peptides with Selective 13C and 15N Isotopic Labels  (Danting Huang, Benjamin C. Hudson, Yuan Gao, Evan K. Roberts, Anant K. Paravastu)....Pages 23-68
 ATR-FTIR Analysis of Amyloid Proteins  (Jean-Marie Ruysschaert, Vincent Raussens)....Pages 69-81
 Imaging Protein Fibers at the Nanoscale and In Situ  (Angelo Bella, Michael Shaw, Emiliana De Santis, Maxim G. Ryadnov)....Pages 83-100
 Replica Exchange Molecular Dynamics: A Practical Application Protocol with Solutions to Common Problems and a Peptide Aggregation and Self-Assembly Example  (Ruxi Qi, Guanghong Wei, Buyong Ma, Ruth Nussinov)....Pages 101-119
 An Aggregate Weight-Normalized Thioflavin-T Measurement Scale for Characterizing Polymorphic Amyloids and Assembly Intermediates  (Ronald Wetzel, Saketh Chemuru, Pinaki Misra, Ravi Kodali, Smita Mukherjee, Karunakar Kar)....Pages 121-144
 Nanoparticle Tracking for Protein Aggregation Research  (Xiaomeng Lu, Regina M. Murphy)....Pages 145-158
 Peptide Self-Assembly Measured Using Fluorescence Correlation Spectroscopy  (Judith J. Mittag, Joachim O. Rädler, Jennifer J. McManus)....Pages 159-171
Front Matter ....Pages 173-173
 A General Method to Prepare Peptide-Based Supramolecular Hydrogels  (Dan Yuan, Junfeng Shi, Ning Zhou, Bing Xu)....Pages 175-180
 Recursive Directional Ligation Approach for Cloning Recombinant Spider Silks  (Nina Dinjaski, Wenwen Huang, David L. Kaplan)....Pages 181-192
 Synthesis of Mikto-Arm Star Peptide Conjugates  (Jin Mo Koo, Hao Su, Yi-An Lin, Honggang Cui)....Pages 193-207
 Synthesis and Evaluation of Self-Assembled Nanostructures of Peptide-π Chromophore Conjugates  (Tejaswini S. Kale, John D. Tovar)....Pages 209-220
 Programmable Fabrication of Multilayer Collagen Nanosheets of Defined Composition  (Tao Jiang, Vincent P. Conticello)....Pages 221-232
 Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies  (Carolina Mora Solano, Yi Wen, Huifang Han, Joel H. Collier)....Pages 233-248
 Microwave-Assisted Synthesis and Immunological Evaluation of Self-Assembling Peptide Vaccines  (C. Brent Chesson, Rojelio Elias Alvarado, Jai S. Rudra)....Pages 249-259
 Preparation and Screening of Catalytic Amyloid Assemblies  (Zsofia Lengyel, Caroline M. Rufo, Ivan V. Korendovych)....Pages 261-270
 Self-assembly of Filamentous Cell Penetrating Peptides for Gene Delivery  (Dawei Xu, Louis DeRidder, Brendan Elmore, He Dong)....Pages 271-281
 Biogelx: Cell Culture on Self-Assembling Peptide Gels  (Mhairi M. Harper, Michael L. Connolly, Laura Goldie, Eleanore J. Irvine, Joshua E. Shaw, Vineetha Jayawarna et al.)....Pages 283-303
Front Matter ....Pages 305-305
 Production and Use of Recombinant Aβ for Aggregation Studies  (Tiernan T. O’Malley, Sara Linse, Dominic M. Walsh)....Pages 307-320
 Disaggregation of Aβ42 for Structural and Biochemical Studies  (Hyewon Chung, Elliot J. Crooks, Martine Ziliox, Steven O. Smith)....Pages 321-330
 Preparation of Stable Amyloid-β Oligomers Without Perturbative Methods  (Samuel A. Kotler, Ayyalusamy Ramamoorthy)....Pages 331-338
 Discriminating Strains of Self-Propagating Protein Aggregates Using a Conformational Stability Assay  (Heather H. C. Lau, Angus Lau, Joel C. Watts)....Pages 339-354
 Model Phospholipid Liposomes to Study the β-Amyloid-Peptide-Induced Membrane Disruption  (Wei Qiang, Katelynne E. Doherty)....Pages 355-367
 Using Molecular Tweezers to Remodel Abnormal Protein Self-Assembly and Inhibit the Toxicity of Amyloidogenic Proteins  (Ravinder Malik, Jing Di, Gayatri Nair, Aida Attar, Karen Taylor, Edmond Teng et al.)....Pages 369-386
 Incorporation of an Azobenzene β-Turn Peptidomimetic into Amyloid-β to Probe Potential Structural Motifs Leading to β-Sheet Self-Assembly  (Todd M. Doran, Bradley L. Nilsson)....Pages 387-406
 Solid-State NMR Studies of Amyloid Materials: A Protocol to Define an Atomic Model of Aβ(1–42) in Amyloid Fibrils  (Yiling Xiao, Dan McElheny, Minako Hoshi, Yoshitaka Ishii)....Pages 407-428
 Experimental and Computational Protocols for Studies of Cross-Seeding Amyloid Assemblies  (Baiping Ren, Rundong Hu, Mingzhen Zhang, Yonglan Liu, Lijian Xu, Binbo Jiang et al.)....Pages 429-447
Back Matter ....Pages 449-452