PCR Topics: Usage of Polymerase Chain Reaction in Genetic and Infectious Diseases

Front Matter....Pages I-XI
Front Matter....Pages 1-1
Quantitation of mRNA by the Polymerase Chain Reaction....Pages 3-8
Sequencing of PCR Products — Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes....Pages 9-14
Alternative Methods for DNA Probing in Diagnosis: Ligase Chain Reaction (LCR)....Pages 15-22
Application of PCR to the Detection and Analysis of Point Mutations in the Human Factor VIII Gene....Pages 23-31
The Use of PCR in the Diagnosis of Muscle Disease....Pages 32-40
Taq DNA Polymerase-Synthesized Single-Stranded DNA Hybridization Probes and their Application in Northern Blotting and in situ Hybridization....Pages 41-45
Computer Program for the Selection of PCR Primers....Pages 46-47
Nervous System Cross Reactivity Sequences....Pages 48-52
The Use of Short Adapters for Priming PCR of Unknown Chromosomal DNA Fragments....Pages 53-55
Labeling of Specific DNA Sequences with Digoxigenin during Polymerase Chain Reaction....Pages 56-58
Quantitative Analysis of Polymerase Chain Reaction (PCR) Products by Means of Fluorescence Detection....Pages 59-62
Reverse Transcription PCR (RT-PCR) of Parathyroid Hormone (PTH) mRNA....Pages 63-65
False Positive Reactions in PCR....Pages 66-68
Error Production and Error Propagation During PCR....Pages 69-71
Front Matter....Pages 73-73
PCR in Linkage Analysis of Genetic Diseases....Pages 75-79
Amplification, Isolation and Direct Nucleotide Determination of Entire Genes: Application to the Study of 16S rRNAs for Molecular Evolution in Bacteria, Identification of Cultural Isolates and Development of Probes....Pages 80-83
Diagnosis of Cystic Fibrosis — Detection of the Gene Deletion Delta F 508....Pages 84-88
Application of PCR for Prenatal Diagnosis of Cystic Fibrosis (CF)....Pages 89-91
The Use of Non-Viable Cultured Amniotic Fluid Cells for Prenatal Diagnosis of Cystic Fibrosis by Polymerase Chain Reaction....Pages 92-93
Determination of Glutathione S-Transferase u Genotype in Humans by PCR-Amplified Gene-Probes....Pages 94-98
Front Matter....Pages 73-73
Rapid Apolipoprotein E Genotype Identification....Pages 99-102
Front Matter....Pages 103-103
Detection of Herpes Viruses by PCR-Diagnostic Aspects....Pages 105-112
Diagnosis of Herpes Simplex Virus Encephalitis (HSVE) by Polymerase Chain Reaction....Pages 113-116
Rapid Detection of CMV-Specific DNA and mRNA by PCR in Immunocompromised Patients....Pages 117-123
Specific and Sensitive Detection of Cytomegalovirus DNA in Clinical Specimens by Polymerase Chain Reaction....Pages 124-129
Detection of EBNA2-mRNA in Primary Central Nervous System Lymphomas in Patients with AIDS....Pages 130-135
Detection of Epstein-Barr Virus Genomes in Lymphoproliferative Diseases....Pages 136-141
Screening of Lymphatic Tissues for the Presence of Human Herpesvirus 6 DNA Using the Polymerase Chain Reaction (PCR)....Pages 142-144
HTLV Retrovirus in Neurological Disease....Pages 147-160
Use of PCR to Investigate the Cellular Tropism of Human T Cell Leukemia Virus Type 1....Pages 161-170
Viral Genome Sequences in Cerebrospinal Fluid and Serum of HIV-I-Infected but Asymptomatic Patients....Pages 171-177
PCR Detection of HBV Replication in Anti-HBeAg Chronic Carriers with Pre-S Antigenemia....Pages 181-185
Detection of Virus DNA by PCR in Hepatitis Non-A, Non-B....Pages 186-191
Hepatitis B Virus DNA — Identification by Polymerase Chain Reaction (PCR) and Correlation with Serological HBV Markers....Pages 192-195
Front Matter....Pages 197-197
Rapid Diagnosis of Mycobacterium Tuberculosis Infection by PCR....Pages 199-202
Detection of Chlamydia Trachomatis by PCR ....Pages 203-205
The Polymerase Chain Reaction (PCR) to Detect Gene Sequences of Borrelia Burgdorferi, the Etiologic Agent of Lyme Disease....Pages 206-208
Rapid Diagnosis of Human Listeriosis Using the Polymerase Chain Reaction....Pages 209-213
Front Matter....Pages 215-215
Quantitation of Mdr1 Transcript by PCR a Tool for Monitoring Drug Resistance in Cancer Chemotherapy....Pages 217-220
Ras Oncogene Mutations in Renal Cell Cancer....Pages 221-223
Front Matter....Pages 215-215
Primer Mismatch Analysis: a Rapid and Non-Isotopic Method for Analysing Ras Mutations in Human Cancer....Pages 224-229
Expression of Adenovirus Type 12 13S Gene Product is Sufficient to Immortalize Primary Mouse Cells....Pages 230-233
PCR-Based Rapid Detection of the Philadelphia-Chromosome in Chronic Myelogenous and Acute Lymphoblastic Leukemia....Pages 234-238
Back Matter....Pages 239-258