PCR Technology: Principles and Applications for DNA Amplification

Front Matter....Pages i-x
Front Matter....Pages 1-5
The Design and Optimization of the PCR....Pages 7-16
Taq DNA Polymerase....Pages 17-22
PCR Automation....Pages 23-30
Simple and Rapid Preparation of Samples for PCR....Pages 31-38
Front Matter....Pages 39-43
Direct Sequencing of In Vitro Amplified DNA....Pages 45-60
Using PCR to Engineer DNA....Pages 61-70
Mutation Detection by PCR, GC-Clamps, and Denaturing Gradient Gel Electrophoresis....Pages 71-88
Detection of Gene Expression....Pages 89-97
PCR Amplification of Specific Sequences from a cDNA Library....Pages 99-104
Inverse Polymerase Chain Reaction....Pages 105-111
Alu PCR: The Use of Repeat Sequence Primers for Amplification of Human DNA from Complex Sources....Pages 113-118
A New Approach to Constructing Genetic Maps: PCR Analysis of DNA Sequences in Individual Gametes....Pages 119-135
Evolutionary Analysis via PCR....Pages 137-147
Front Matter....Pages 149-151
Use of PCR in the Diagnosis of Monogenic Disease....Pages 153-169
Diagnosis of New Mutation Diseases Using the Polymerase Chain Reaction....Pages 171-191
HLA Class II Gene Polymorphism: DNA Typing, Evolution, and Relationship to Disease Susceptibility....Pages 193-208
Applications of PCR to the Analysis of Biological Evidence....Pages 209-223
Detection of ras Oncogenes Using PCR....Pages 225-233
Application of PCR to the Detection of Human Infectious Diseases....Pages 235-244
Back Matter....Pages 245-246