Pancreatic Cancer: Methods and Protocols

Front Matter ....Pages i-xii
 Subtyping of IPMN  (Toru Furukawa)....Pages 1-8
 Clinical Management and Surgical Decision-Making of IPMN of the Pancreas  (Masao Tanaka)....Pages 9-22
 Develop Nomogram to Predict Malignancy of Intraductal Papillary Mucinous Neoplasm  (Yongkang Kim, Taesung Park)....Pages 23-32
 Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal Adenocarcinoma  (Jungsun Kim, Kenneth S. Zaret)....Pages 33-53
 Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar-to-Ductal Metaplasia  (Jun Liu, Pei Wang)....Pages 55-62
 Spheroid Culture of Human Pancreatic Ductal Cells to Reconstitute Development of Pancreatic Intraepithelial Neoplasia  (James J. Lee, Seung K. Kim)....Pages 63-71
 Pancreatic Microtumors: A Novel 3D Ex Vivo Testing Platform  (Mackenzie Lee Goodwin, Sumi Kanthraj Urs, Diane M. Simeone)....Pages 73-80
 3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering  (Claudio Ricci, Serena Danti)....Pages 81-95
 Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens  (Lindsey A. Baker, Hervé Tiriac, David A. Tuveson)....Pages 97-115
 Generation and Culture of Tumor and Metastatic Organoids from Murine Models of Pancreatic Ductal Adenocarcinoma  (Lindsey A. Baker, David A. Tuveson)....Pages 117-133
 An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding  (Takashi Okumura, Kenoki Ohuchida, Masafumi Nakamura)....Pages 135-141
 Osteoclast Differentiation Assay  (Jingxuan Yang, Xiaohong Bi, Min Li)....Pages 143-148
 Primary Cultures for Pancreatic Stellate Cells (PSCs)  (Lei Tian, Zipeng Lu, Yi Miao)....Pages 149-155
 Cytokine Profiling and Orthotopic Xenografing of Pancreatic Stellate Cells  (Dong Qian, Lei Tian, Zipeng Lu, Yi Miao)....Pages 157-160
 Quantitative Method to Track Proteolytic Invasion in 3D Collagen  (Kazumi Ebine, Christina R. Chow, Hidayatullah G. Munshi)....Pages 161-169
 Detection and Quantification of Macropinosomes in Pancreatic Tumors  (Szu-Wei Lee, Basheer Alas, Cosimo Commisso)....Pages 171-181
 Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo  (Edward Sim, Elena Irollo, Elda Grabocka)....Pages 183-195
 Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells  (Chiara Vidoni, Alessandra Ferraresi, Christian Seca, Eleonora Secomandi, Ciro Isidoro)....Pages 197-206
 Measurement of Reactive Oxygen Species by Fluorescent Probes in Pancreatic Cancer Cells  (Yongde Luo, Dan Wang, James L. Abbruzzese, Weiqin Lu)....Pages 207-219
 Evaluating the Metabolic Alterations in Pancreatic Cancer  (Aneesha Dasgupta, Surendra K. Shukla, Venugopal Gunda, Ryan J. King, Pankaj K. Singh)....Pages 221-228
 Isolation of Extracellular Vesicles for Cancer Diagnosis and Functional Studies  (Alex W. Brenner, Gloria H. Su, Fatemeh Momen-Heravi)....Pages 229-237
 Evaluation of Post-transcriptional Gene Regulation in Pancreatic Cancer Cells: Studying RNA Binding Proteins and Their mRNA Targets  (Aditi Jain, Samantha Z. Brown, Henry L. Thomsett, Eric Londin, Jonathan R. Brody)....Pages 239-252
 Laser Capture Microdissection on Frozen Sections for Extraction of High-Quality Nucleic Acids  (H. Carlo Maurer, Kenneth P. Olive)....Pages 253-259
 Statistical Methods for Identifying Biomarkers from miRNA Profiles of Cancers  (Junghyun Namkung)....Pages 261-286
 Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice  (Lu Zhuang, Xianbao Zhan, Yan Bi, Baoan Ji)....Pages 287-297
 Pancreatic Acinar-to-Ductal Metaplasia and Pancreatic Cancer  (Liang Wang, Dacheng Xie, Daoyan Wei)....Pages 299-308
 Orthotopic Pancreatic Tumor Mouse Models of Liver Metastasis  (George Zhang, Yi-Chieh Nancy Du)....Pages 309-320
 Molecular and Physiological Evaluation of Pancreatic Cancer-Induced Cachexia  (Surendra K. Shukla, Aneesha Dasgupta, Scott E. Mulder, Pankaj K. Singh)....Pages 321-333
Back Matter ....Pages 335-337