Organtransplantation in Rats and Mice: Microsurgical Techniques and Immunological Principles

Front Matter....Pages I-XXIII
Front Matter....Pages 1-1
History and Development of Experimental Microsurgery....Pages 3-8
Organ Transplantation in Mice: Current Status and Future Prospects....Pages 9-15
Experimental Microsurgery as an Educational Concept and Scientific Basis for a Research Group: Principles of the Technical Development of Animal Models in Transplantation Research....Pages 17-24
Front Matter....Pages 25-25
Guidelines for the Use and Care of Small Laboratory Animals in Transplantation Research....Pages 27-40
Technical Aids and Training Program for Microsurgery....Pages 41-46
Statistical Methods in Small Animal Experimentation....Pages 47-54
In Vitro Analysis of Transplantation Experiments in Small Animals: Personal Experience and Practical Aspects....Pages 55-59
Front Matter....Pages 61-61
Microsurgical Techniques for Vascular Anastomoses: Suture and Cuff Technique....Pages 63-67
Heart Transplantation....Pages 69-72
Techniques of Skin, Renal, and Vascular Transplantation in the Rat....Pages 73-81
Microsurgical Techniques for Small Bowel Transplantation in Rats....Pages 83-96
Technique of Rat Islet Transplantation....Pages 97-104
Pancreas Transplantation in Rats....Pages 105-111
Combined Pancreas and Kidney Transplantation....Pages 113-122
Liver Transplantation....Pages 123-131
Lung and Thoracic Heart-Lung Transplantation....Pages 133-140
Combined Abdominal Organ Transplantation....Pages 141-147
Front Matter....Pages 149-149
Surgical Technique for Skin Transplantation in Mice....Pages 151-154
Surgical Technique for Vascularized Ear Transplantation in Mice....Pages 155-160
A Microsurgical Technique for Orthotopic Aorta Transplantation in Mice....Pages 161-165
Front Matter....Pages 149-149
Pancreatic Islet and Proislet Transplantation in the Mouse Model....Pages 167-177
Vascular Thymus Transplantation in Mice....Pages 179-184
Surgical Techniques for Mouse Heart Transplantation....Pages 185-192
Microsurgical Techniques for Kidney Transplantation in Mice....Pages 193-201
Microsurgical Techniques for Small Bowel Transplantation in Mice....Pages 203-211
Surgical Techniques for Orthotopic Liver Transplantation in Mice....Pages 213-221
Front Matter....Pages 223-223
Concepts and Mechanisms of Immune Activation, Graft Rejection, Immunosuppression, and Tolerance....Pages 225-252
Immunogenetic Basis of Organ Graft Rejection in Rat and Mouse....Pages 253-276
Oral Feeding and Intrathymic Injection of MHC Class I-derived Allopeptides Prolong Graft Survival....Pages 277-284
Microchimerism in Organ Transplantation....Pages 285-298
Gene Transfer and Tolerance Induction....Pages 299-312
Lessons from Knockout and Transgenic Mice....Pages 313-324
Immunological Methods in Small Animal Research....Pages 325-337
Analytical Methods in Molecular Biology....Pages 339-350
Front Matter....Pages 351-351
Venovenous Allotransplantation in the Rat....Pages 353-358
Segmental Femoral Artery Allografts: Histomorphological Analysis of the Rejection Response in Inbred Rat Strains....Pages 359-364
Chronic Rejection of Vascularized Organ Grafts in Rodent Models....Pages 365-373
Immunosuppression in Experimental Heart Transplantation....Pages 375-384
The Use of Rat Heterotopic Heart Transplantation Models to Characterize the Immunosuppressive Activities of Leflunomide....Pages 385-397
Lymphatic Reconstruction after Intestinal Transplantation in Rats....Pages 399-405
Front Matter....Pages 407-407
Immunological Phenomenon after Small Bowel Transplantation....Pages 409-416
Mucosal Immunology of the Small Bowel....Pages 417-425
Function Studies After Small Bowel Transplantation in the Rat....Pages 427-433
Methods and Effects of Immunosuppression After Small Bowel Transplantation in the Rat....Pages 435-439
Macrophage Depletion in Small Animal Transplant Models....Pages 441-451
Combined Liver and Small Bowel Transplantation — The Tolerogenic Effect on the Liver....Pages 453-464
Front Matter....Pages 465-465
Immunological Basis of Pancreas Transplantation — Experimental Studies in the Rat....Pages 467-474
Immunosuppression After Pancreas Transplantation....Pages 475-480
Graft-Versus-Host Reaction After Pancreas Transplantation in the Rat....Pages 481-487
Management of Exocrine Secretion in Experimental Pancreas Transplantation in Rats....Pages 489-500
Results After Islet Transplantation in Rats....Pages 501-513
Alginate-Encapsulated Islets in Rats....Pages 515-523
Front Matter....Pages 525-525
Orthotopic Liver Transplantation in the Rat: Survival Relationship Between Different Strain Combinations....Pages 527-534
Selective Immunosuppression After Orthotopic Rat Liver Transplantation with Monoclonal Antibodies Directed Against Adhesion Molecules and Activation Antigens....Pages 535-543
Biological Effect of Heterotopic Liver Transplantation....Pages 545-554
Immune Mechanisms of Liver Transplantation Tolerance in the Rat....Pages 555-558
Front Matter....Pages 559-559
Experimental Concordant Xenografting in Small Animals....Pages 561-573
Reduction of Xenoreactive Antibodies by the Pharmacon Antigen Variation Effect in a Discordant Xenotransplantation Model....Pages 575-579
Technique of Plasmapheresis in the Xenogeneic Rat Model....Pages 581-588
Preconditioning Bone Marrow Transplants for Successful Xenotransplantation in the Small Animal Model....Pages 589-594
Front Matter....Pages 559-559
Transgenic Mouse Models and Knockout Mouse Models to Manipulate the Xenograft Response....Pages 595-604
Novel Mouse Models for Xenotransplantation Research....Pages 605-614
Transplantation Models in Human/Mouse Chimeras....Pages 615-626
Front Matter....Pages 627-627
Development of Graft Vascular Disease in Transplanted Aortic Segments in Mice....Pages 629-635
Results of Heart Transplantation in Mice....Pages 637-647
Intestinal Transplantation in the Murine Model....Pages 649-652
Renal Allografts in the Mouse and Donor-Specific Tolerance Induced by Antibody to CD45RB....Pages 653-658
Donor-Specific Tolerance in Mouse Liver Transplantation....Pages 659-665
Back Matter....Pages 667-671