Opioid Receptors: Methods and Protocols

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
 Overview of Genetic Analysis of Human Opioid Receptors  (Santi M. Spampinato)....Pages 3-13
 Renilla Luciferase Reporter Assay to Study 3′-UTR-Driven Posttranscriptional Regulation of OPRM1  (Gabriele Vincelli, Andrea Bedini)....Pages 15-26
 Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1  (Vittoria Borgonetti, Nicoletta Galeotti)....Pages 27-34
 Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)  (Monica Baiula)....Pages 35-43
 Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Nonvisual Arrestins  (Andrea Bedini)....Pages 45-58
 Functionalization and Bioconjugation of Nanoruby for Long-Term, Ultrasensitive Imaging of Μu-Opioid Receptors  (Rashmi Pillai, Mark Connor, Varun K. A. Sreenivasan)....Pages 59-70
 Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues  (Yvonne Schmidt, Halina Machelska)....Pages 71-82
 Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells  (Melih Özgür Celik, Dominika Labuz, Halina Machelska)....Pages 83-95
Front Matter ....Pages 97-97
 Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-Tolerant Mice  (Vittoria Borgonetti, Nicoletta Galeotti)....Pages 99-108
 GTPγS-Autoradiography for Studies of Opioid Receptor Functionality  (Alfhild Grönbladh, Mathias Hallberg)....Pages 109-116
 Evaluating Opioid-Mediated Adenylyl Cyclase Inhibition in Live Cells Using a BRET-Based Assay  (Preeti Manandhar, Shivani Sachdev, Marina Santiago)....Pages 117-125
 Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury  (Viola Seitz, Philip Stötzner, Dominika Labuz, Halina Machelska)....Pages 127-137
 Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV  (Bradley Nash, Elena Irollo, Renato Brandimarti, Olimpia Meucci)....Pages 139-162
 An in Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion  (Monica Baiula)....Pages 163-169
 Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat  (Alfhild Grönbladh, Erik Nylander, Sofia Zelleroth, Mathias Hallberg)....Pages 171-180
 The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls, and Future Perspectives of Biased Agonists at Opioid Receptors  (Andrea Bedini)....Pages 181-192
Front Matter ....Pages 193-193
 Mechanical Nociception in Mice and Rats: Measurement with Automated von Frey Equipment  (Gabriele Campana, Roberto Rimondini)....Pages 195-198
 Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment  (Silvia Franchi, Mara Castelli, Sarah Moretti, Alberto Panerai, Paola Sacerdote)....Pages 199-207
 Measurement of Macrophage Toll-Like Receptor 4 Expression After Morphine Treatment  (Mara Castelli, Alberto Panerai, Paola Sacerdote, Silvia Franchi)....Pages 209-217
Front Matter ....Pages 219-219
 Conditioned Place Preference (CPP) in Rats: From Conditioning to Reinstatement Test  (Maria Scherma, Liana Fattore, Walter Fratta, Paola Fadda)....Pages 221-229
 Analysis of Opioid-Seeking Behavior Through the Intravenous Self-Administration Reinstatement Model in Rats  (Liana Fattore, Paola Fadda, Mary Tresa Zanda, Walter Fratta)....Pages 231-245
 Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors in Rats and Mice  (Roberto Rimondini, Gabriele Campana)....Pages 247-251
 Early-Life Stress as a Probe to Study the Opioid System in Developing Rodents  (Stefano Loizzo, Alberto Loizzo)....Pages 253-258
 Evaluation of μ-Opioid System Functionality in Mouse Pups: Ultrasonic Vocalizations as an Index of Infant Attachment  (Francesca R. D’Amato)....Pages 259-265
Back Matter ....Pages 267-270