Nucleic Acids and Protein Synthesis Part F

Content: 
Contributors to volume XXX
Pages xi-xv

Preface
Page xvii
Kivie Moldave, Lawrence Grossman

Volumes in series
Pages xix-xxi

[1] Chain initiation factors from Escherichia coli Original Research Article
Pages 3-18
A.J. Wahba, M.J. Miller

[2] Electron microscopic studies of chain initiation complexes and polysomes Original Research Article
Pages 19-24
C. Vasquez, M.J. Miller, A.J. Wahba

[3] Purification and phosphorylation of initiation factor IF2 Original Research Article
Pages 24-31
John L. Fakunding, Jolinda A. Traugh, Robert R. Traut, John W.B. Hershey

[4] Preparation and properties of crystalline initiation factor 1 (IF1) from Escherichia coli Original Research Article
Pages 31-39
Sylvia Lee-Huang, Severo Ochoa

[5] Preparation of radioactive initiation factor 3 Original Research Article
Pages 39-44
Steven Sabol, Severo Ochoa

[6] Purification of two messenger-discriminating species of initiation factor 3 (IF3) from Escherichia coli Original Research Article
Pages 45-53
Sylvia Lee-Huang, Severo Ochoa

[7] Selection of ribosome binding sites on mRNA in Escherichia coli by cistron-specific IF3 subfractions, and interference factors Original Research Article
Pages 54-68
Michel Revel, Yoram Groner

[8] Purification and properties of interference factor i: a cistron-specific translation control factor and a subunit of phage RNA replicase Original Research Article
Pages 68-78
Yoram Groner, Michel Revel

[9] The initial steps in protein synthesis: Effects by antibiotics Original Research Article
Pages 79-86
Juan Modolell

[10] Preparation and assay of ribosome dissociation factors from Escherichia coli and rabbit reticulocytes and the assay for free ribosomes Original Research Article
Pages 87-94
Michael Gottlieb, Nicolette H. Lubsen, Bernard D. Davis

[11] Protein chain initiation in wheat embryo Original Research Article
Pages 94-101
Abraham Marcus, Samarendra N. Seal, Donald P. Weeks

[12] Initiation of globin synthesis: Assays Original Research Article
Pages 101-128
Ronald G. Crystal, Norton A. Elson, W. French Anderson

[13] Preparation of protein synthesis initiation factors IF-M1, IF-M2A, and IF-M2B from rabbit reticulocytes Original Research Article
Pages 128-136
William Merrick, Hermann Graf, W. French Anderson

[14] Preparation of rabbit reticulocyte initiation factor IF-M3 Original Research Article
Pages 136-141
Philip M. Prichard, W. French Anderson

[15] Purification and properties of the peptide chain initiation factors from rabbit reticulocytes Original Research Article
Pages 141-153
Charles L. Woodley, Yang C. Chen, Naba K. Gupta

[16] Purification and assays for the presence and biological functions of two eukaryotic initiation factors from rabbit reticulocytes that are required for initiation complex formation with rabbit reticulocyte ribosomes Original Research Article
Pages 153-171
Linda M. Cashion, Geraldine L. Dettman, Wendell M. Stanley Jr.

[17] Preparation of protein synthesis initiation factors from rabbit liver Original Research Article
Pages 171-180
Dante Picciano, W. French Anderson

[18] Isolation, purification, and assay of an initiation factor from rat liver cytosol that promotes binding of aminoacyl-tRNA to 40 S ribosomal subunits Original Research Article
Pages 180-186
David P. Leader, Ira G. Wool

[19] Assay of reassociation of eukaryotic ribosomal subunits catalyzed by initiation factors Original Research Article
Pages 186-197
Richard E.H. Wettenhall, Ira G. Wool

[20] Purification of eukaryotic initiation factor 1 (EIF1) from Artemia saliva embryos Original Research Article
Pages 197-206
Michael Zasloff, Severo Ochoa

[21] Assay and isolation of a 40 S ribosomal subunit В· Met-tRNAfMet binding factor from rabbit reticulocytes Original Research Article
Pages 206-215
J.M. Cimadevilla, B. Hardesty

[22] Elongation factor Tu and the aminoacyl-tRNA В· EFTu В· GTP complex Original Research Article
Pages 219-232
David Lee Miller, Herbert Weissbach

[23] Elongation factor T-dependent GTP hydrolysis: Dissociation from aminoacyl-tRNA binding Original Research Article
Pages 232-235
J.P.G. Ballesta

[24] The binding of Escherichia coli elongation factor G to the ribosome Original Research Article
Pages 235-238
James W. Bodley, Herbert Weissbach, Nathan Brot

[25] Binding of the elongation factors EF 1 and EF 2 to 80 S ribosomes in a cell-free system from porcine brain of the hypothalamic region Original Research Article
Pages 238-245
Dietmar Richter

[26] Separation of cytoplasmic and mitochondrial elongation factors from yeast Original Research Article
Pages 245-253
Dietmar Richter, Fritz Lipmann

[27] Soluble protein factors and ribosomal subunits from yeast. Interactions with aminoacyl-tRNA Original Research Article
Pages 254-261
A. ToraГ±o, A. Sandoval, C.F. Heredia

[28] The use of inhibitors in studies of protein synthesis Original Research Article
Pages 261-282
Sidney Pestka

[29] The Elongation steps in protein synthesis by eukaryotic ribosomes: Effects of antibiotics Original Research Article
Pages 282-289
L. Carrasco, E. Battaner, D. Vazquez

[30] Mammalian release factor; in Vitro Assay and Purification Original Research Article
Pages 293-303
C.T. Caskey, A.L. Beaudet, W.P. Tate

[31] An assay for protein chain termination using peptidyl-tRNA Original Research Article
Pages 303-310
John R. Menninger, Charline Walker

[32] Preparation and characterization of free and membrane-bound polysomes Original Research Article
Pages 313-327
H. Bloemendal, E.L. Benedetti, W.S. Bont

[33] Pressure-induced dissociation of ribosomes Original Research Article
Pages 328-345
R. Baierlein, A.A. Infante

[34] The dissociation of rat liver ribosomes by formamide : Extraction of a 5 S RNAВ·protein complex Original Research Article
Pages 346-349
Mary L. Petermann

[35] Preparation of ribosomal subunits by large-scale zonal centrifugation Original Research Article
Pages 349-354
Paul S. Sypherd, John W. Wireman

[36] Separation of large quantities of eukaryotic ribosomal subunits by zonal ultracentrifugation Original Research Article
Pages 354-367
Corinne C. Sherton, Ralph F. Di Camelli, Ira G. Wool

[37] A general procedure for the preparation of highly active eukaryotic ribosomes and ribosomal subunits Original Research Article
Pages 368-387
Glenn E. Brown, Alfred J. Kolb, Wendell M. Stanley Jr.

[38] The characterization of ribosomal subunits of eukaryotes by ultracentrifugal techniques. Criteria of purity Original Research Article
Pages 387-395
Mary G. Hamilton

[39] Hydrogen-tritium exchange studies on ribosomes Original Research Article
Pages 395-406
Melvin V. Simpson

[40] The inactivation and reactivation of Escherichia coli ribosomes Original Research Article
Pages 406-426
Ada Zamir, Ruth Miskin, Zvi Vogel, David Elson

[41] preparation of [3H]anisomycin and [3H]gougerotin: Binding to eukaryotic ribosomes Original Research Article
Pages 426-439
Mariano Barbacid, David Vazquez

[42] Ribonuclease sensitivity of aminoacyl-tRNA: an assay for codon recognition and interaction of aminoacyl-tRNA with 50 S subunits Original Research Article
Pages 439-452
Sidney Pestka

[43] “Nonenzymatic” translation Original Research Article
Pages 452-462
L.P. Gavrilova, A.S. Spirin

[44] Assay for nonenzymatic and enzymatic translocation with Escherichia coli ribosomes Original Research Article
Pages 462-470
Sidney Pestka

[45] Peptidyl-puromycin synthesis on polyribosomes from Escherichia coli Original Research Article
Pages 470-479
Sidney Pestka

[46] Peptidyl-puromycin synthesis on polyribosomes from rat liver or brain Original Research Article
Pages 479-488
Sidney Pestka

[47] Assay of ester and polyester formation by the ribosomal peptidyltransferase Original Research Article
Pages 489-497
Stephen Fahnestock, Helmut Neumann, Alexander Rich

[48] Two-dimensional polyacrylamide gel electrophoresis for separation of ribosomal proteins Original Research Article
Pages 497-505
H.G. Wittmann

[49] Two-dimensional polyacrylamide gel electrophoresis of eukaryotic ribosomal proteins Original Research Article
Pages 506-526
Corinne C. Sherton, Ira G. Wool

[50] A modified two-dimensional gel system for the separation and radioautography of microgram amounts of ribosomal proteins Original Research Article
Pages 526-539
G.A. Howard, R.R. Traut

[51] Estimation of the number and size of ribosomal proteins by two-dimensional gel electrophoresis Original Research Article
Pages 540-545
Mary G. Hamilton

[52] Determination of ribosomal protein molecular weight distributions from stained dodecyl sulfate gels Original Research Article
Pages 545-553
T.A. Bickle, R.R. Traut

[53] Constitution of 50 S ribosomal subunits from Bacillus stearothermophilus Original Research Article
Pages 554-562
S. Fahnestock, V. Erdmann, M. Nomura

[54] Isotopic labeling and analysis of phosphoproteins from mammalian ribosomes Original Research Article
Pages 563-590
Lawrence Bitte, David Kabat

[55] Phosphorylation of ribosomal proteins: preparation of rat liver ribosomal protein kinases. Assay of the phosphorylation of ribosomal subunit proteins. Assay of the function of phosphorylated ribosomes Original Research Article
Pages 590-601
Charles Eil, Ira G. Wool

[56] The isolation of messenger RNA from mammalian cells Original Research Article
Pages 605-612
George Brawerman

[57] Isolation of rabbit reticulocyte 9 S mRNA Original Research Article
Pages 613-621
Bernard Lebleu

[58] Preparation of globin messenger RNA Original Research Article
Pages 621-630
Arthur W. Nienhuis, Amy K. Falvey, W. French Anderson

[59] Immunoadsorption of ovalbumin synthesizing polysomes and partial purification of ovalbumin messenger RNA Original Research Article
Pages 631-648
Robert T. Schimke, Rafael Palacios, Drew Sullivan, Mary L. Kiely, Carmen Gonzales, John M. Taylor

[60] The purification of the messenger RNA for silk fibroin Original Research Article
Pages 648-654
Donald D. Brown, Yoshiaki Suzuki

[61] DNA- and RNA-directed synthesis in vitro of phage enzymes Original Research Article
Pages 654-669
Peter Herrlich, Manfred Schweiger

[62] Cell-free synthesis of myosin Original Research Article
Pages 669-674
Stuart M. Heywood, Arthur W. Rourke

[63] Translation of mRNA from vertebrate eye lenses Original Research Article
Pages 675-694
Anton J.M. Berns, Hans Bloemendal

[64] Assay of ovalbumin mRNA in reticulocyte lysate Original Research Article
Pages 694-701
Robert T. Schimke, Robert E. Rhoads, G. Stanley McKnight

[65] Isolation on cellulose of ovalbumin and globin mRNA and their translation in an ascites cell-free system Original Research Article
Pages 701-708
Gunther Schutz, Miguel Beato, Philip Feigelson

[66] Translation of reovirus mRNA, poliovirus RNA, and bacteriophage QОІ RNA in cell-free extracts of mammalian cells Original Research Article
Pages 709-723
Lydia Villa-Komaroff, Maxson McDowell, David Baltimore, Harvey F. Lodish

[67a] Protein synthesis with rabbit reticulocyte preparations Original Research Article
Pages 724-731
William R. Woodward, Joel L. Ivey, Edward Herbert

[67b] Preparation and characterization of an inhibitor of globin synthesis from the postribosomal supernatant of reticulocyte lysates Original Research Article
Pages 731-740
William R. Woodward, Edward Herbert

[67c] Preparation of reticulocyte aminoacyl-tRNA and the assay of codon recognition properties of isoacceptor tRNA\'s in a reticulocyte cell-free system Original Research Article
Pages 740-746
William R. Woodward, Prapon Wilairat, Edward Herbert

[67d] Preparation and analysis of nascent chains on reticulocyte membrane-bound ribosomes Original Research Article
Pages 746-748
William R. Woodward, Edward Herbert

[68] The wheat embryo cell-free system Original Research Article
Pages 749-754
Abraham Marcus, Dov Efron, Donald P. Weeks

[69] In vitro translation of STNV-RNA Original Research Article
Pages 754-761
John M. Clark Jr., William H. Klein

Author index
Pages 763-781

Subject index
Pages 782-808