Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology: Practical Protocols

Front Matter....Pages I-XXVI
Front Matter....Pages 1-1
Temperature-Dependent Dimorphism of the Non-Conventional Yeast Arxula adeninivorans ....Pages 3-6
Expression of Heterologous Genes in Arxula adeninivorans Budding Cells and Mycelia....Pages 7-12
Front Matter....Pages 13-13
Effect of Precursor Feeding on Riboflavin Production of Ashbya gossypii and Influence of Threonine Aldolase Overexpression....Pages 15-20
Front Matter....Pages 21-21
Characterization of the 26S-rRNA Gene to Classify an Industrial Strain to be Candida maltosa ....Pages 23-28
Isolation of a GCN4 Gene Analog and Determination of Its Involvement in Induction of Cycloheximide-Resistant Ribosomal Protein L41-Q in Candida maltosa ....Pages 29-34
Dehalogenation During Biodegradation of Chlorophenols by Candida maltosa ....Pages 35-38
Production of Aromatic Acids During the Oxidation of Phenylalkanes by Candida maltosa ....Pages 39-44
Front Matter....Pages 45-45
Growth of Debaryomyces hansenii in Seawater Culture Medium....Pages 47-49
Glycerol and Glucose Dissimilation by Debaryomyces hansenii: Substrate Influence on Growth Rate and Biomass Yield of Yeast....Pages 51-57
Induction of GPD from the Halotolerant Yeast Debaryomyces hansenii by Salt Stress....Pages 59-63
Salt Tolerance in Debaryomyces hansenii . Effect of Sodium on Growth at High Temperature and at Extreme pH....Pages 65-68
Preparation of Total DNA from Debaryomyces hansenii ....Pages 69-73
Front Matter....Pages 75-75
Life Cycle of Hansenula polymorpha ....Pages 77-81
Obtention of Auxotrophic Mutants in Hansenula polymorpha ....Pages 83-88
Transformation by Electroporation of the Yeast Hansenula polymorpha ....Pages 89-96
Expression of Hansenula polymorpha Nitrate Reductase Under the Control of the M0X1 Promoter....Pages 97-100
Promoter Assessment in Hansenula polymorpha Using a lacZ Reporter Gene....Pages 101-115
Immuno-Gold Labeling of Invertase in Hansenula polymorpha ....Pages 117-123
Front Matter....Pages 125-125
Determination of Intra- and Extracellular Concentration of Glycerol in the Halophilic Black Yeast Hortaea werneckii Grown at Different Environmental Salinities....Pages 127-130
Regulation of HMGCoA Reducíase Activity by Salt Stress in Hortaea werneckii ....Pages 131-134
Front Matter....Pages 125-125
Isolation and Identification of Lipids from the Halophilic Black Yeast Hortaea werneckii ....Pages 135-142
Inhibition of DHN-Melanin Biosynthesis by Tricyclazole in Hortaea Werneckii ....Pages 143-148
Front Matter....Pages 149-149
Mating, Sporulation and Tetrad Analysis in Kluyveromyces lactis ....Pages 151-154
Isolation of Mitochondrial Mutants from the Petite-Negative Yeast Kluyveromyces lactis ....Pages 155-159
Gene Disruption in the Yeast Kluyveromyces lactis ....Pages 161-167
Transformation of Kluyveromyces lactis ....Pages 169-174
Removal of Dominant Markers from the Kluyveromyces lactis Genome Using the Cre/loxP System....Pages 175-178
Phenotypic Analysis of the Kluyveromyces lactis Killer Phenomenon....Pages 179-183
Genetic Manipulation of the Kluyveromyces lactis Killer Plasmids k1 and k2....Pages 185-190
Chitin-Binding Capability of the Zymocin Complex from Kluyveromyces lactis ....Pages 191-194
Heterologous Protein Production in High Copy Number Vector Systems....Pages 195-199
Alcohol Dehydrogenase (ADH) Isozymes in Kluyveromyces lactis: Detection by Activity....Pages 201-207
 Kluyveromyces lactis Genome Mapping and Sequencing....Pages 209-211
Front Matter....Pages 213-213
Mating and Segregation in Pichia stipitis ....Pages 215-219
 ADH1 Disruption in Pichia stipitis by Targeted Restriction Enzyme Mediated Integration (tREMI)....Pages 221-228
Examination of Xylose Fermentation in Pichia stipitis by Means of the Sensomat System....Pages 229-233
Regulation of Fermentation and Respiration....Pages 235-240
Investigation of Transcriptional Regulation of the Fermentative ADH in Pichia stipitis Using an EGFP Reporter Gene....Pages 241-244
Cyanide-Insensitive Respiration....Pages 245-252
Freeze Transformation, Plasmid Reisolation and Stability in Pichia stipitis ....Pages 253-259
Front Matter....Pages 261-261
Objective Analysis of Medium-Induced Fragility of Schwanniomyces occidentalis ....Pages 263-266
Subjective Analysis of Medium-Induced Fragility of Schwanniomyces occidentalis ....Pages 267-269
Analysis of Structural Discontinuities of the Wall of Schwanniomyces occidentalis ....Pages 271-274
Induction of Amylases in Schwanniomyces occidentalis ....Pages 275-278
Ethanol Production from Starch by Schwanniomyces occidentalis ....Pages 279-282
Front Matter....Pages 283-283
Characterization of Soluble Cytochrome P450 from Trichosporon domesticum ....Pages 285-290
Front Matter....Pages 291-291
Oxidation of the Fungicide Biphenyl by Yeasts of the Genus Trichosporon ....Pages 293-296
Front Matter....Pages 297-297
Genetic Complementation Analysis by Protoplast Fusion of Xanthophyllomyces dendrorhous ....Pages 299-304
Lethal Effect of UV Light and Photoreactivation in Xanthophyllomyces dendrorhous ....Pages 305-308
Fermentation and Carotenoid Analysis of the Yeast Xanthophyllomyces dendrorhous [Phaffia rhodozyma) ....Pages 309-313
Metabolic Engineering of the Carotenoid Biosynthetic Pathway in Xanthophyllomyces dendrorhous [Phaffia rhodozyma) ....Pages 315-322
Astaxanthin Biosynthesis in Xanthophyllomyces dendrorhous ( Phaffia rhodozyma ): Effect of Inhibitors and Low Temperature....Pages 323-328
Purification and Characterization of Extrachromosomal Genetic Elements of Double-Stranded RNA (dsRNA) of Xanthophyllomyces dendrorhous ....Pages 329-332
Front Matter....Pages 333-333
Mating, Sporulation and Random Spore Selection by Nystatin in Yarrowia lipolytica ....Pages 335-338
Induction of the Yeast-Hypha Transition in Yarrowia lipolytica ....Pages 339-342
Tagging of Genes by Insertional Mutagenesis in the Yeast Yarrowia lipolytica ....Pages 343-356
Detection and Activation of the Ylt1 Retrotransposon in the Yeast Yarrowia lipolytica ....Pages 357-364
Flow Cytometry of Yarrowia lipolytica ....Pages 365-368
A Copper-Regulated Yarrowia lipolytica Vector for the Expression of Heterologous Proteins....Pages 369-371
Lipase Assay in Yarrowia lipolytica ....Pages 373-377
Front Matter....Pages 333-333
Expression of Mutant Gpr1p in Yarrowia lipolytica and Its Effect on Growth on Different Media....Pages 379-384
Detection of Biosynthesis and Degradation of Peroxisomes by Different Carbon Sources with a Combined Assay Using a β-Galactosidase-GFP-SKL Chimaeric Protein for Labeling of Peroxisomes and the Peroxisomal Isocitrate Lyase as Control....Pages 385-392
Induction of Citric Acid/lsocitric Acid and α-Ketoglutaric Acid Production in the Yeast Yarrowia lipolytica ....Pages 393-400
Regulation of Production of Yarrowia lipolytica Extracellular Ribonuclease and Alkaline Extracellular Protease....Pages 401-406
Front Matter....Pages 407-407
 Zygosaccharomyces bailii: A Yeast With a Peculiar Pattern for the Regulation of Acetic Acid Metabolism in the Presence of Glucose....Pages 409-416
Transfection of Yeast With Toxin-Coding dsRNA Viruses of Zygosaccharomyces bailii and Toxin Purification by Receptor-Mediated Affinity Chromatography....Pages 417-425
Front Matter....Pages 427-427
Heat-Shock-Induced Activation of Trehalase in the Yeast Zygosaccharomyces rouxii ....Pages 429-433
Front Matter....Pages 435-435
Linear and Circular Plasmids of Yeasts....Pages 437-443
Rapid Method to Extract DNA from Capsulated Yeast Species....Pages 445-449
Testing Various Yeasts for Ethanol Production from Xylose....Pages 451-455
Use of a Differential Culture Medium for the Enumeration of Zygosaccharomyces bailii, Saccharomyces cerevisiae and Pichia membranifaciens in Wine....Pages 457-462
Characterization of Ubiquinone Type (CoQ7–CoQ10) of Non-Conventional Yeasts....Pages 463-467
Non-Radioactive Dot Blot DNA Reassociation for Unequivocal Yeast Identification....Pages 469-474
Taxonomy Rules and Classification Hints for Non-Taxonomists Working with Unknown or JII-Identified Yeast Cultures....Pages 475-485
Back Matter....Pages 486-494