Neuroendocrine Peptide Methodology

Content: 
SELECTED METHODS IN ENZYMOLOGY, Page ii
Front Matter, Page iii
Copyright, Page iv
List of Contributors, Pages xi-xv
Preface, Page xvii, P. MICHAEL CONN
Contents of Methods in Enzymology, Volumes 103, 124, and 168, Pages xix-xxxi
1 - Regulation of Neuroendocrine Peptide Gene Expression, Pages 3-18, M. BLUM
2 - Gene Transfer Methods for Studying the Regulation and Expression of Neuropeptide Genes, Pages 19-35, AUDREY SEASHOLTZ, MICHAEL COMB, MITCHEL MARTIN, EDWARD HERBERT
3 - Neuropeptide Gene Transcription in Central and Peripheral Nervous System Tissue by Nuclear Run-On Assay, Pages 37-46, JEFFREY D. WHITE, EDMUND F. LAGAMMA
4 - RNA Dot and Blot Hybridization: Selected Procedures for Endocrine and Neuroendocrine Studies, Pages 47-56, BRUCE A. WHITE, THOMAS LUFKIN, GREGORY M. PRESTON, CARTER BANCROFT
5 - Detection of Neuroendocrine Peptide Precursor cDNA Clones Using Synthetic Oligonucleotides, Pages 57-65, JAMES DOUGLASS, EDWARD HERBERT
6 - Measurement of Neuroendocrine Peptide mRNA in Discrete Brain Regions, Pages 67-78, JEFFREY D. WHITE, KIM D. STEWART, JEFFREY F. McKELVY
7 - High-Resolution in Situ Hybridization Histochemistry, Pages 79-95, FRANK BALDINO JR., MARIE-FRANCOISE CHESSELET, MICHAEL E. LEWIS
8 - Nonradioactive Methods of in Situ Hybridization: Visualization of Neuroendocrine mRNA, Pages 97-105, P.C. EMSON, H. ARAI, S. AGRAWAL, C. CHRISTODOULOU, M.J. GAIT
9 - Graphical and Statistical Approaches to Data Analysis for in Situ Hybridization, Pages 107-133, JOSEPH T. McCABE, ROBERT A. DESHARNAIS, DONALD W. PFAFF
10 - In Situ Hybridization: Quantitation Using Radiolabeled Hybridization Probes, Pages 135-146, GEORGE R. UHL
11 - Quantitation and Digital Representation of in Situ Hybridization Histochemistry, Pages 147-160, MICHAEL E. LEWIS, WADE T. ROGERS, RUDOLPH G. KRAUSE II, JAMES S. SCHWABER
12 - Measurement of Neuropeptide Release: In Vitro and in Vivo Procedures, Pages 163-191, J.E. LEVINE, V.D. RAMIREZ
13 - Measurement of Hormone Secretion from Individual Cells by Cell Blot Assay, Pages 193-204, MARCIA E. KENDALL, W.C. HYMER
14 - Detection and Measurement of Secretion from Individual Neuroendocrine Cells Using a Reverse Hemolytic Plaque Assay, Pages 205-227, PHILIP F. SMITH, ENRIQUE H. LUQUE, JIMMY D. NEILL
15 - Use of Microdialysis in Neuroendocrinology, Pages 229-252, KEITH M. KENDRICK
16 - Push–Pull Perfusion Technique in the Median Eminence: A Model System for Evaluating Releasing Factor Dynamics, Pages 253-264, NORMAN W. KASTING, JOSEPH B. MARTIN
17 - Intracellular Calcium Levels in Rat Anterior Pituitary Cells: Single-Cell Techniques, Pages 267-288, DENIS A. LEONG
18 - Measurement of Intracellular Ionized Calcium with Aequorin, Pages 289-314, ANDRÉ B. BORLE, KENNETH W. SNOWDOWNE
19 - Use of Intracellular Calcium and Membrane Potential Fluorescent Indicators in Neuroendocrine Cells, Pages 315-340, ALESSANDRO M. CAPPONI, P. DANIEL LEW, WERNER SCHLEGEL, TULLIO POZZAN
20 - Transient Permeabilization of Endocrine Cells: Inositol Lipid Metabolism, Pages 341-348, IRENE WINICOV, MARVIN C. GERSHENGORN
21 - Use of Protein Kinase C-Depleted Cells for Investigation of the Role of Protein Kinase C in Stimulus–Response Coupling in the Pituitary, Pages 349-363, CRAIG A. McARDLE, P. MICHAEL CONN
22 - Synthesis and Use of Diacylglycerols as Activators of Protein Kinase C in Neuroendocrine Tissue, Pages 365-370, P. MICHAEL CONN, BARRY R. GANONG, JAMES EBELING, DAPHNE STALEY, JAMES E. NIEDEL, ROBERT M. BELL
23 - Assay and Purification by Conventional Column Chromatography of Protein Kinase C, Pages 371-374, TATSURO KITANO, MASAYOSHI GO, USHIO KIKKAWA, YASUTOMI NISHIZUKA
24 - Mixed Micelle Assay of Protein Kinase C, Pages 375-381, ROBERT M. BELL, YUSUF HANNUN, CARSON LOOMIS
25 - A Rapid Method for the Resolution of Protein Kinase C Subspecies from Rat Brain Tissue, Pages 383-387, MARK S. SHEARMAN, KOUJI OGITA, USHIO KIKKAWA, YASUTOMI NISHIZUKA
26 - Comparative Methods for the Radiolabeling of Peptides, Pages 391-401, A.E. BOLTON
27 - Application of Photoaffinity Labeling to Neuroendocrine Tissues, Pages 403-416, ELI HAZUM
28 - Preparation and Application of Biotinylated Neuroendocrine Peptides, Pages 417-427, ELI HAZUM
29 - Synthesis and Use of Colloidal Gold-Coupled Receptor Ligands, Pages 429-440, LOTHAR JENNES, P. MICHAEL CONN, WALTER E. STUMPF
30 - Luciferin Derivatization of Ligands for Development of Nonisotopic Binding Assays, Pages 441-447, JAMES M. SCHAEFFER
31 - Single-Channel Electrophysiology: Use of the Patch Clamp, Pages 451-479, FREDERICK SACHS, ANTHONY AUERBACH
32 - Patch Clamp Recording from Anterior Pituitary Cells Identified by Reverse Hemolytic Plaque Assay, Pages 481-502, THOMAS L. CROXTON, WILLIAM McD. ARMSTRONG, NIRA BEN-JONATHAN
33 - Temperature Measurement and Control of Small Volumes: Applications for Single Channel Recording, Pages 503-518, ANTHONY AUERBACH, FREDERICK SACHS, JAMES NEIL, RICHARD McGARRIGLE
34 - Electrophysiological Assays of Mammalian Cells Involved in Neurohormonal Communication, Pages 519-537, BERNARD DUFY, LUCE DUFY-BARBE, JEFFERY L. BARKER
35 - Electrophysiological Techniques in Dissociated Tissue Culture, Pages 539-559, GARY L. WESTBROOK, PHILLIP G. NELSON
36 - Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells, Pages 561-596, W.T. MASON, C.D. INGRAM
37 - Use of Specific Ion Channel Activating and Inhibiting Drugs in Neuroendocrine Tissue, Pages 597-601, P. MICHAEL CONN
38 - Strategies for the Preparation of Haptens for Conjugation and Substrates for Iodination for Use in Radioimmunoassay of Small Oligopeptides, Pages 605-617, WILLIAM W. YOUNGBLOOD, JOHN STEPHEN KIZER
39 - High-Density Multiple Antigen–Peptide System for Preparation of Antipeptide Antibodies, Pages 619-627, JAMES P. TAM
40 - Development and Use of Ultrasensitive Enzyme Immunoassays, Pages 629-654, GLENNWOOD E. TRIVERS, CURTIS C. HARRIS, CATHERINE ROUGEOT, FERNAND DRAY
41 - Production of Monoclonal Antibodies Reacting with the Cytoplasm and Surface of Differentiated Cells, Pages 655-664, RICHARD M. SCEARCE, GEORGE S. EISENBARTH
42 - Preparation and Use of Specific Polyclonal and Monoclonal Antibodies for Immunohistochemistry of Neuropeptides, Pages 665-678, LOTHAR JENNES, WALTER E. STUMPF
43 - Immunocytochemistry of Endorphins and Enkephalins, Pages 679-696, FLOYD E. BLOOM, ELENA L.F. BATTENBERG
44 - Preparation and Use of Complementary Peptides, Pages 697-709, KENNETH L. BOST, J. EDWIN BLALOCK
45 - Application of Fast Atom Bombardment Mass Spectrometry to Posttranslational Modifications of Neuropeptides, Pages 713-744, P.C. ANDREWS, JACK E. DIXON
46 - Flow Cytometry as an Analytic and Preparative Tool for Studies of Neuroendocrine Function, Pages 745-762, JOHN C. CAMBIER, JOHN G. MONROE
47 - Computer-Controlled Perifusion System for Neuroendocrine Tissues: Development and Applications, Pages 763-775, ANDRES NEGRO-VILAR, MICHAEL D. CULLER
48 - Visualization of Enkephalin Receptors by Image-Intensified Fluorescence Microscopy, Pages 777-785, STEVEN G. BLANCHARD, KWEN-JEN CHANG, PEDRO CUATRECASAS
49 - Cell-to-Cell Communication in Peptide Target Cells of Anterior Pituitary, Pages 787-811, CARL DENEF, PHILIPPE MAERTENS, WILFRIED ALLAERTS, ANNICK MIGNON, WIM ROBBERECHT, LUC SWENNEN, PETER CARMELIET
50 - Synthesis and Use of Neuropeptides, Pages 815-844, JOHN M. STEWART
51 - Microisolation of Neuropeptides, Pages 845-862, FRED S. ESCH, NICHOLAS C. LING, PETER BÖHLEN
52 - Solubilization of Neuropeptide Receptors, Pages 863-870, MARILYN H. PERRIN
53 - Punch Sampling Biopsy Technique, Pages 871-879, MIKLÓS PALKOVITS
54 - Use of Excitatory Amino Acids to Make Axon-Sparing Lesions of Hypothalamus, Pages 881-890, J. VICTOR NADLER, DEBRA A. EVENSON
55 - Excitotoxic Amino Acids as Neuroendocrine Research Tools, Pages 891-905, JOHN W. OLNEY, MADELON T. PRICE
56 - Simultaneous Localization of Steroid Hormones and Neuropeptides in the Brain by Combined Autoradiography and Immunocytochemistry, Pages 907-914, MADHABANANDA SAR, WALTER E. STUMPF
57 - Techniques for Tracing Peptide-Specific Pathways, Pages 915-921, LARRY W. SWANSON
Index, Pages 923-949