Neurocytochemical Methods

Front Matter....Pages I-XVI
Development and Application of Antibodies to Primary (DA, L-DOPA) and Secondary (cGMP) Messengers: A technical report....Pages 1-27
Transmitter-Related Retrograde Labeling and Immunohistochemistry to Localize Amino Acid Transmitters in Neural Pathways and Cells....Pages 29-44
Ultrastructural Identification and Quantification of Neuroactive Amino Acids....Pages 45-57
Video-Microscopic Techniques to Study Axonal Transport, Neuronal Fine Structure and Cytochemical Parameters in Live Neurons....Pages 59-73
Investigating the Three-Dimensional Morphology of Fluorescence-Labelled Neurons Using Confocal Laser Scanning Microscopy....Pages 75-87
Summary of the Session on Hodology....Pages 89-92
Combined Cytolabeling Methods for Investigating Chemo-Specific Neuronal Interactions and Transmitter Co-Localization....Pages 93-116
Molecular Neuroanatomy of Synapses, Cells and Systems in the Brain....Pages 117-135
Regulated Expression of Transcription Factor and Neurotransmitter Genes in Neural Populations: Studies Using In Situ Hybridization with Radiolabelled Oligonucleotide Probes....Pages 137-142
The Use of Markers to Study the Regulation of Glial Cells in the PNS and CNS....Pages 143-152
Report on the Discussion of the Second Session: Detection of Specific Molecules, and the Second Plenary Lecture: Molecular Neuroanatomy of Synapses, Cells and Systems in the Brain....Pages 153-167
Applications to the Study of Acetylcholine Release of the Choline Oxidase Acetylcholine Assay....Pages 169-182
Morphometric Evaluation of Populations of Neuronal Profiles in the Central Nervous System....Pages 183-195
Positron Emission Tomography: Functional Neuroanatomy In Vivo ....Pages 197-207
Microfluorometric Imaging of Time and Space Dependent Calcium Concentration Changes in Mammalian Neurons....Pages 209-221
Report on the Discussion of the Third Session: Quantitative Aspects....Pages 223-224
Immunocytochemistry of Neuropeptide Receptors using Anti-Idiotypic Antibodies and Antibodies to Peptides Encoded by Complementary RNA....Pages 225-240
New Approaches for Receptor Analysis: Histochemistry of the GABA/BZ Receptors in the Rat CNS....Pages 241-262
Report on the Discussion of the Fourth Session: Receptors....Pages 263-265
Extracellular Dyes Induce Photodegeneration and Photopermeabilization of Vertebrate and Invertebrate Neurones....Pages 267-271
A Method to Study Projections of Gabaergic Neurons Using Deletion of the Gaba Immunostaining by Metallic Ions....Pages 273-277
Ultrastructural Immunocytochemical Localization of Choline Acetyltransferase in Sympathetic Ganglia....Pages 279-282
A Dual Immunoperoxidase Labelling Method to Study the Ultrastructural Relationships between Choline Acetyltransferase- and Neuropeptide Y-Containing Neurons in the Rat Striatum....Pages 283-288
Characterization of Microglia and Brain Macrophages....Pages 289-293
Visualization and Quantification of 5-HT 1A Receptors in the Rat Brain by Radioimmunohistochemistry....Pages 295-299
Anti-Idiotypic Antibodies against Opioid Receptors: First Ultrastructural Evidence for a Presynaptic Inhibition of Opioids in the Dorsal Horn of the Rat Spinal Cord....Pages 301-305
Antibodies to Complementary Peptide for Labelling Substance P Receptors in the Dorsal Horn of the Rat Spinal Cord for both Light and Electron Microscopy....Pages 307-311
Comparative Ultrastructural Localization of Neuropeptide mRNAs using Tritiated and Biotinylated Oligodeoxynucleotide Probes....Pages 313-318
Use of In Situ Hybridization and Immunohistochemistry to Study the Effects of Intranigral Injections of Colchicine on Enkephalin- and Substance P-Containing Neurons of the Limbic Forebrain....Pages 319-323
Report on the Poster Session....Pages 325-328
Back Matter....Pages 329-335