Mouse Genetics After The Mouse Genome: Cytogenetic And Genome Research 2004

Contents......Page 4
Preface......Page 6
Duration of meiosis......Page 7
Spreads and antibody localization......Page 8
Mouse testis sections and synchronization of meiotic prophase events......Page 9
Antibody localization on knock-out mice......Page 10
References......Page 11
Abstract......Page 13
Fluorescence in situ hybridisation......Page 14
Immunostained two-dimensional spreads......Page 15
Locus-specific FISH on cryosections......Page 16
Cryosections......Page 18
Acknowledgements......Page 21
References......Page 22
Abstract......Page 23
SYCP3 and SYCP2 are required for chromosome compaction......Page 26
Summary......Page 27
References......Page 29
Historical perspective and goals of this review......Page 30
What happens as a result of vitamin A deficiency in testis?......Page 31
What happens to vitamin A after ingestion?......Page 33
Role of retinoid-specific binding proteins......Page 34
Discovery of the receptors gave insight into the mechanisms by which the metabolites of retinol could exert their functions......Page 35
Control of cell cycle progression by retinoids......Page 36
Cell-cell interactions affected by loss of RARa mediated signaling......Page 37
Serum binding proteins......Page 38
Unanswered questions......Page 39
References......Page 40
General overview......Page 44
Testis-pecific TH2B......Page 45
Novel H2B-like proteins in human germline cells......Page 46
MacroH2A and XY body formation......Page 47
The histone H1 family......Page 48
Spermatocyte-specific linker histone H1t......Page 49
The histone complement in human sperm......Page 50
Conclusions......Page 52
References......Page 53
Abstract......Page 56
PCR primers......Page 57
Cpt1b,Cpt2 and Hprt expression during preimplantation development......Page 58
Quantitative analysis of Cpt2 expression evidences the presence of two groups of embryos throughout preimplantation development......Page 59
Cpt1b,Cpt2 and Hprt expression in antral and ovulated oocytes......Page 60
References......Page 61
Abstract......Page 63
The mutagenesis and phenotype screening program at the Jackson Laboratory......Page 64
From mutant models to contraception......Page 66
Conclusions and perspective......Page 67
References......Page 68
Abstract......Page 69
Characteristics of mouse ZP glycoproteins......Page 70
Organization and expression of mouse ZP genes......Page 71
Synthesis and secretion of mouse ZP glycoproteins......Page 72
Final comments......Page 73
References......Page 74
Abstract......Page 76
Results......Page 77
Discussion......Page 79
References......Page 80
Abstract......Page 81
Whole-mount immunostaining......Page 82
The tool:cDNA library to ESTs to transcriptome......Page 83
Expression of mobile elements:regulatory aspects......Page 84
Annotation of the 2-cell embryo transcriptome......Page 86
References......Page 90
Abstract......Page 92
2-cell embryos......Page 93
4-cell embryos......Page 95
Acknowledgements......Page 96
References......Page 97
Placental development and function......Page 98
Expression profiling approaches......Page 99
Growth factors and growth factor receptors......Page 100
Members of the signal transduction cascade......Page 102
Transcription factors......Page 104
Evolutionary and comparative aspects of placentogenesis......Page 106
References......Page 107
Abstract......Page 111
Determination of total ATP content in single preimplantation embryos......Page 112
Influence of culture medium on the ATP profile of clone embryos......Page 113
Discussion......Page 115
References......Page 118
Abstract......Page 120
Glucose uptake and glucose transporter expression......Page 121
Alterations in cytoplasm-nucleus protein trafficking......Page 122
SCC effects on the loss of H1 linker histone exchange capacity......Page 123
Conclusions......Page 124
References......Page 125
Introduction......Page 126
Abnormal gene expression in cloned animals......Page 127
Reprogramming of DNA methylation and imprinting......Page 128
Histone modifications in cloned animals......Page 129
Telomere restoration in clones......Page 130
References......Page 131
Abstract......Page 133
Microscopy and quantitative analysis......Page 134
Peripherization of centromeres during T-cell differentiation......Page 135
Relative positioning of CD4 and CD8 loci......Page 137
Centromeric proximity of CD4 and CD8......Page 138
Discussion......Page 139
References......Page 141
Abstract......Page 143
Microscopy......Page 144
Results......Page 145
Changes of nuclear architecture during terminal PC differentiation......Page 149
References......Page 151
Agouti viable yellow......Page 152
Axin-fused......Page 153
Stochastic re-establishment of epigenetic state......Page 154
Mitotic and meiotic memory......Page 155
References......Page 156
Abstract......Page 157
DNA methylation in Primordial Germ Cells (PGCs)......Page 158
Late prenatal and neonatal periods......Page 161
Adults......Page 162
Acknowledgements......Page 163
References......Page 164
DNA methylation and gene function......Page 166
CpG islands with T-DMR in normal tissues and cells......Page 167
Genome-wide DNA methylation profiles focusing on T-DMR of CpG islands......Page 169
Abnormal DNA methylation status in cloned animals......Page 171
Mechanism for formation and maintenance of DNA methylation patterns......Page 172
References......Page 173
Abstract......Page 176
Screens based on imprinted expression......Page 177
Screens based on epigenetic features......Page 178
Location and organization of imprinted genes......Page 179
CpG islands and direct repeats......Page 180
Factors marking ICEs......Page 181
Regulation by antisense transcription......Page 182
Long-range regulation......Page 183
References......Page 184
Abstract......Page 187
Endogenous RT activity is required for mouse early embryonic development......Page 188
Inhibition of RT activity reduces cell proliferation and promotes differentiation in progenitor and transformed cell types......Page 189
Concluding remarks......Page 190
References......Page 191
Abstract......Page 192
Influence of the genomic context......Page 193
Placenta......Page 194
Differentiating cells and tissues......Page 195
ERVs and immunity......Page 196
Agents that disrupt cell functions......Page 197
Endocrine modulation of ERVs......Page 198
Conclusions......Page 199
References......Page 201
Abstract......Page 204
Network reconstruction using Bayesian networks......Page 206
Consensus networks......Page 208
Assessing the significance of predicted networks......Page 209
Sub-networks and cis-acting QTL......Page 211
Linkage disequilibrium (LD)as a confounding factor in the reconstruction of gene networks......Page 212
Discussion......Page 214
References......Page 215
The thanks to – in spite of paradox......Page 216
The molecular machinery of fusion......Page 217
Effectiveness of the post-mating barrier......Page 219
The parapatric contact area......Page 221
The selective advantage of the chromosomal novelty......Page 222
References......Page 223
Abstract......Page 226
II. Mouse evolutionary genetics: selection, speciation......Page 227
Evolution of reproductive isolation......Page 228
Genetic introgression,selection and evolution......Page 230
III. Mouse as a mammal: evolution of isochores......Page 231
IV. Mouse biodiversity and immune response......Page 232
References......Page 233
Coat colors, chromosomes and valleys full of mice......Page 236
More chromosomes and more coat colors......Page 239
The origin of the metacentric races and the particular importance of hybridization......Page 242
The “ tail” does not end here......Page 244
References......Page 245
Abstract......Page 247
Results......Page 248
Discussion......Page 249
The ancestral rodent genome......Page 250
References......Page 251
Abstract......Page 253
RNAi delivery: how to reach the target ?......Page 254
Knock-down versus knock-out......Page 256
Epi-allelic series: RNAi as a tool for generating graded loss-of-function......Page 257
Caveats and limitations: To be or not to be on target?......Page 259
Perspective......Page 260
References......Page 261
The PKR/ interferon pathway......Page 263
The RNAi pathway......Page 264
The mammalian RNAi pathway does not utilize RNA dependent RNA polymerase......Page 265
The role of RNAi in mammalian cells......Page 266
Experimental gene silencing with long dsRNA in mammalian cells......Page 267
dsRNA microinjection of oocytes and early embryos......Page 269
Transgenic RNAi......Page 270
Acknowledgements......Page 273
References......Page 274
Abstract......Page 276
Small Fragment Homologous Replacement (SFHR)......Page 277
Triplex-forming oligonucleotides......Page 279
References......Page 281
HMG proteins......Page 283
Overexpression studies......Page 284
Acknowledgements......Page 286
References......Page 287
Modifier genes......Page 289
The genetic background influences the test results obtained with fragile X knockout mice......Page 290
Morris water maze......Page 291
Neuroanatomy......Page 292
Conclusion and prospects......Page 293
References......Page 294
What is replicative senescence?......Page 296
Testing the link between senescence and ageing: methods to abrogate senescence......Page 297
The tumour suppressor and checkpoint protein p53 links telomere erosion to cell cycle arrest......Page 298
The role of p16 INK4A in senescence......Page 299
Telomere-independent senescence: slow cell division counters......Page 300
Murine models of replicative senescence......Page 301
Acknowledgements......Page 302
References......Page 303
Abstract......Page 305
Telomere fluorescence intensity in mouse lymphoma cell lines......Page 306
Telomere fluorescence variation in individual cells......Page 307
Telomere fluorescence and colcemid treatment time......Page 308
Discussion......Page 310
References......Page 311
Telomere structure......Page 312
Telomere replication......Page 313
Telomerase-deficient mice......Page 314
The Ku 86 and DNA-PKcs-deficient mice......Page 315
Ku86-deficient mice......Page 316
References......Page 317
Characteristics of the mouse model......Page 320
Therapeutic approaches: lessons from mouse research......Page 321
Therapeutic approaches: lessons from mouse research......Page 322
Conclusions......Page 323
References......Page 324
Author Index Vol. 105, No. 2–4, 2004......Page 326
Author Index Vol. 105, 2004......Page 327
Contents Vol. 105, 2004......Page 328