Molecular and Cellular Aspects of Erythropoietin and Erythropoiesis

Front Matter....Pages I-VIII
Preface....Pages 1-4
Acknowledgements....Pages 5-5
Abbreviations....Pages 6-8
Front Matter....Pages 9-9
The Molecular Biology of Erythropoietin and the Expression of its Gene....Pages 11-21
The Molecular Biology of Erythropoietin....Pages 23-36
Analysis of Erythroid Cell-Specific Gene Expression....Pages 37-50
The Role of Oxygen Tension in the Regulation of Protein Synthesis in Erythroid Cells....Pages 51-60
Erythropoietin Production and Erythropoietin Receptors on Murine Erythroleukemic Cell Lines....Pages 61-72
The Purification of Spleen CFU-E and its Applications in the Study of In Vitro Erythropoiesis....Pages 73-87
Use of Purified Erythropoietin Responsive Cells Produced by the Anemia Strain of Friend Virus to Study the Action of Erythropoietin....Pages 89-102
Retroviral Vectors for Gene Transfer and Expression in Haematopoietic Cells....Pages 103-121
Back Matter....Pages 122-125
Front Matter....Pages 126-126
Respective Roles of Programme and Differentiation Factors during Hemoglobin Switching in the Embryo....Pages 127-145
Cellular Interactions and/or Random Differentiation for the Formation of Erythroid Cells in the Early Chick Embryo....Pages 147-163
Divergence of Erythroid Restricted Precursors from Hemopoietic Stem Cells During Early Ontogeny....Pages 165-175
Some Endocrine Aspects of Renal and Extrarenal Erythropoietin in Normoxic and Hypoxic Rats....Pages 177-186
Perturbation of Erythropoiesis during the Period of Early Anemia. A Model for Studying the Regulation of Erythropoiesis in the Neonatal Mammal....Pages 187-204
The Cellular Basis of Developmental Changes in Haemoglobin Synthesis....Pages 205-223
Back Matter....Pages 225-226
Front Matter....Pages 227-227
Cell to Cell Interaction in Erythropoiesis: The Role of the Thymus....Pages 229-236
Erythropoiesis and Macrophage Subsets in Medullary and Extramedullary Sites....Pages 237-258
Studies on the Interaction between Murine Resident Bone Marrow Macrophages and Haematopoietic Cells....Pages 259-272
Back Matter....Pages 372-375
Front Matter....Pages 227-227
Monocyte-Macrophage Mediated Suppression of Erythropoiesis in Renal Anemia....Pages 273-289
Erythropoietin Production by Macrophages: Cellular Response to Physiological Oxygen Tensions and Detection of Erythropoietin Gene Expression by In Situ Hybridization....Pages 291-310
Chemoreception of Oxygen in the Kidney and Erythropoietin Production....Pages 311-327
Renal Biogenesis of Erythropoietin....Pages 329-336
Enhanced Erythropoietic Response to Hypobaria in Hypertransfused, Post-Hypoxic, Post-Anemic or Testosterone-Treated Polycythemic Rodents....Pages 337-351
The Use of Two-Phase Systems for the Fractionation of Heterogeneous Populations of Bone Marrow Cells and Erythrocytes: Bisphosphoglycerate Mutase as an Enzyme Marker for Erythroid Cells....Pages 353-371
Back Matter....Pages 372-375
Front Matter....Pages 376-376
The Estimation of Erythropoietin (Epo): Principles, Problems and Progress....Pages 377-387
The Use of 125 I Recombinant DNA Derived Human Erythropoietin (R-HuEPO) as a Replacement for 125 I Human Urinary Epo as Tracer Antigen in a Radioimmunoassay for Human Epo.....Pages 389-393
Development of a Radioimmunoassay for Erythropoietin Using Recombinant Erythropoietin-Derived Reagents....Pages 395-407
Isolation of Anti-Erythropoietin by Automated Immuno-Affinity FPLC using Recombinant EPO....Pages 409-416
The Effect of Heat-Treatment on the Estimation of Bioactive Erythropoietin in Serum using the Mouse Spleen Cell Assay....Pages 417-428
Standards for the Assay of Erythropoietin....Pages 429-438
Back Matter....Pages 439-441
Back Matter....Pages 442-461