Microtubules,

Content: 
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xiii-xviii

Preface
Pages xix-xx
John (Jack) Correia, Leslie Wilson

Chapter 1 - Site-Specific Fluorescent Labeling of Tubulin
Pages 1-12
Kamalika Mukherjee, Susan L. Bane

Chapter 2 - Measuring Microtubule Persistence Length Using a Microtubule Gliding Assay
Pages 13-25
Douglas S. Martin

Chapter 3 - Structural Studies of the Doublecortin Family of MAPs
Pages 27-48
Franck Fourniol, Mylène Perderiset, Anne Houdusse, Carolyn Moores

Chapter 4 - Detection and Quantification of Microtubule Detachment from Centrosomes and Spindle Poles
Pages 49-62
Anutosh Ganguly, Hailing Yang, Fernando Cabral

Chapter 5 - Regulation of Tubulin Expression by Micro-RNAs: Implications for Drug Resistance
Pages 63-74
Sharon Lobert, Mary E. Graichen

Chapter 6 - Determining the Structure–Mechanics Relationships of Dense Microtubule Networks with Confocal Microscopy and Magnetic Tweezers-Based Microrheology
Pages 75-96
Yali Yang, Megan T. Valentine

Chapter 7 - Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes
Pages 97-108
Andrea Pelikan, James Sillibourne, Stephanie Miserey-Lenkei, Frederique Carlier-Grynkorn, Bruno Goud, Phong T. Tran

Chapter 8 - Going Solo: Measuring the Motions of Microtubules with an In Vitro Assay for TIRF Microscopy
Pages 109-124
Kris Leslie, Niels Galjart

Chapter 9 - Analysis of Microtubules in Isolated Axoplasm from the Squid Giant Axon
Pages 125-137
Yuyu Song, Scott T. Brady

Chapter 10 - Imaging GTP-Bound Tubulin: From Cellular to In Vitro Assembled Microtubules
Pages 139-153
Hélène de Forges, Antoine Pilon, Christian Poüs, Franck Perez

Chapter 11 - Tubulin-Specific Chaperones: Components of a Molecular Machine That Assembles the α/β Heterodimer
Pages 155-171
Guoling Tian, Nicholas J. Cowan

Chapter 12 - Heterotrimeric G Proteins and Microtubules
Pages 173-189
Witchuda Saengsawang, Mark M. Rasenick

Chapter 13 - Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro
Pages 191-213
Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L. Ross

Chapter 14 - Measurement of In Vitro Microtubule Polymerization by Turbidity and Fluorescence
Pages 215-229
Matthew Mirigian, Kamalika Mukherjee, Susan L. Bane, Dan L. Sackett

Chapter 15 - Live-Cell Imaging of Microtubules and Microtubule-Associated Proteins in Arabidopsis thaliana
Pages 231-246
Jessica Lucas

Chapter 16 - Investigating Tubulin Posttranslational Modifications with Specific Antibodies
Pages 247-267
Maria M. Magiera, Carsten Janke

Chapter 17 - Purification and Assembly of Bacterial Tubulin BtubA/B and Constructs Bearing Eukaryotic Tubulin Sequences
Pages 269-281
José M. Andreu, María A. Oliva

Chapter 18 - Microtubule-Associated Proteins and Tubulin Interaction by Isothermal Titration Calorimetry
Pages 283-302
P.O. Tsvetkov, P. Barbier, G. Breuzard, V. Peyrot, F. Devred

Chapter 19 - Methods for Studying Microtubule Binding Site Interactions: Zampanolide as a Covalent Binding Agent
Pages 303-325
Jessica J. Field, Enrique Calvo, Peter T. Northcote, John H. Miller, Karl-Heinz Altmann, José Fernando Díaz

Chapter 20 - Studying Kinetochore-Fiber Ultrastructure Using Correlative Light-Electron Microscopy
Pages 327-342
Daniel G. Booth, Liam P. Cheeseman, Ian A. Prior, Stephen J. Royle

Chapter 21 - Fluorescence-Based Assays for Microtubule Architecture
Pages 343-354
Susanne Bechstedt, Gary J. Brouhard

Chapter 22 - Structure–Function Analysis of Yeast Tubulin
Pages 355-374
Anna Luchniak, Yusuke Fukuda, Mohan L. Gupta Jr.

Chapter 23 - Using MTBindingSim as a Tool for Experimental Planning and Interpretation
Pages 375-384
Julia T. Philip, Aranda R. Duan, Emily O. Alberico, Holly V. Goodson

Chapter 24 - Imaging Individual Spindle Microtubule Dynamics in Fission Yeast
Pages 385-394
Judite Costa, Chuanhai Fu, Viktoriya Syrovatkina, Phong T. Tran

Index
Pages 395-405

Volumes in Series
Pages 407-417