Microbial Toxins: Tools in Enzymology

Content: 
Contributors to volume 165
Pages ix-xii

Preface
Page xiii
Sidney Harshman

Volumes in series
Page xv

Methods in enzymology
Pages xvii-xxix

[1] Preparation and purification of staphylococcal alpha toxin Original Research Article
Pages 3-7
Sidney Harshman, Nancy Sugg, Paul Cassidy

[2] Preparation and purification of Оі-hemolysin of staphylococci Original Research Article
Pages 8-16
Michel Plommet

[3] Purification and assay of staphylococcal Оґ-lysin Original Research Article
Pages 16-22
T Harry Birkbeck, John H Freer

[4] Purification and crystallization of staphylococcal leukocidin Original Research Article
Pages 22-32
Masatoshi Noda, Iwao Kato

[5] Purification of epidermolytic toxin of Staphylococcus aureus Original Research Article
Pages 32-36
Joyce C.S de Azavedo, Christopher J Bailey, John P Arbuthnott

[6] Preparation of toxic shock syndrome toxin-1 Original Research Article
Pages 37-43
Debra A Blomster-Hautamaa, Patrick M Schlievert

[7] Purification of staphylococcal enterotoxins Original Research Article
Pages 43-52
John J Iandolo, Rodney K Tweten

[8] Production, purification, and assay of streptolysin O Original Research Article
Pages 52-59
Joseph E Alouf, Christiane Geoffroy

[9] Production, purification, and assay of streptolysin S Original Research Article
Pages 59-64
Joseph E Alouf, Catherine Loridan

[10] Production, purification, and assay of streptococcal erythrogenic toxin Original Research Article
Pages 64-67
Christiane Geoffroy, Joseph E Alouf

[11] Diphtheria toxin: Purification and properties Original Research Article
Pages 68-76
Stephen F Carroll, Joseph T Barbieri, R John Collier

[12] Isolation and characterization of the Botulinum neurotoxins Original Research Article
Pages 76-85
Lance L Simpson, James J Schmidt, John L Middlebrook

[13] Purification of tetanus toxin and its major peptides Original Research Article
Pages 85-90
John P Robinson

[14] Purification of alpha toxin from clostridium perfringens: Phospholipase C Original Research Article
Pages 91-94
Colette Jolivet-Reynaud, HervГ© Moreau, Joseph E Alouf

[15] Production, purification, and assay of Clostridium perfringens enterotoxin Original Research Article
Pages 94-103
James L McDonel, Bruce A McClane

[16] Production and purification of anthrax toxin Original Research Article
Pages 103-116
Stephen H Leppla

[17] Production and isolation of neissereal IgA proteases Original Research Article
Pages 117-120
Andrew G Plaut

[18] Purification of pertussis toxin Original Research Article
Pages 120-126
V.Y Perera

[19] Purification of Escherichia coli heat-stable enterotoxin Original Research Article
Pages 126-137
Richard N Greenberg, Abdul M.K Saeed

[20] Purification of Escherichia coli О±-hemolysin Original Research Article
Pages 137-146
G.A Bohach, S.J Cavalieri, I.S Snyder

[21] Preparation of Pseudomonas exotoxin A Original Research Article
Pages 147-152
Kenneth J Kozak, Catharine B Saelinger

[22] Shiga toxin: Production and purification Original Research Article
Pages 152-162
Gerald T Keusch, Arthur Donohue-Rolfe, Mary Jacewicz, Anne V Kane

[23] Preparation of Yersinia pestis plague murine toxin Original Research Article
Pages 162-168
Thomas C Montie

[24] Production and purification of cholera toxin Original Research Article
Pages 169-175
John J Mekalanos

[25] Cytolytic toxins of Vibrio vulnificus and Vibrio damsela Original Research Article
Pages 176-189
Arnold S Kreger, Mahendra H Kothary, Larry D Gray

[26] Thermostable direct hemolysin of Vibrio parahaemolyticus Original Research Article
Pages 189-193
Yoshifumi Takeda

[27] Aerolysin from Aeromonas hydrophila Original Research Article
Pages 193-199
J Thomas Buckley, S Peter Howard

[28] Preparation of hemolysin from Aeromonas Original Research Article
Pages 200-203
Åsa Ljungh, Torkel Wadström

[29] Preparation of diphtheria toxin fragment a coupled to hormone Original Research Article
Pages 204-210
Thomas N Oeltmann, Ronald G Wiley

[30] Assay of hemolytic toxins Original Research Article
Pages 213-217
Alan W Bernheimer

[31] Diphtheria toxin: Quantification and assay Original Research Article
Pages 218-225
Stephen F Carroll, R John Collier

[32] Use of exotoxin A to inhibit protein synthesis Original Research Article
Pages 226-231
Catharine B Saelinger

[33] Shiga toxin as inhibitor of protein synthesis Original Research Article
Pages 231-235
Arthur Donohue-Rolfe, Mary Jacewicz, Gerald T Keusch

[34] [32P]ADP-ribosylation of proteins catalyzed by cholera toxin and related heat-labile enterotoxins Original Research Article
Pages 235-245
D Michael Gill, Marilyn Woolkalis

[35] Assay and purification of cytosolic factor required for cholera toxin activity Original Research Article
Pages 246-249
Marilyn Woolkalis, D Michael Gill, Jenifer Coburn

Selective destruction of vagal sensory neurons using Shigella cytotoxin Original Research Article
Pages 250-254
Ronald G Wiley, Arthur Donohue-Rolfe, Gerald T Keusch, Thomas N Oeltmann

[37] Measurement of neurotoxic actions on mammalian nerve impulse conduction Original Research Article
Pages 254-269
Jonathan J Lipman

[38] Electroencephalogram as a tool for assaying neurotoxicity Original Research Article
Pages 270-277
Jonathan J Lipman

[39] Assay of pore-forming toxins in cultured cells using radioisotopes Original Research Article
Pages 278-285
Monica Thelestam

[40] Incorporation of toxin pores into liposomes Original Research Article
Pages 285-293
Roswitha FГјssle, JГёrgen Tranum-Jensen, Andreas Sziegoleit, Sucharit Bhakdi

[41] Assay methods for alpha toxin from Clostridium perfringens: Phospholipase C Original Research Article
Pages 293-297
Colette Jolivet-Reynaud, HervГ© Moreau, Joseph E Alouf

[42] Phospholipase activity of bacterial toxins Original Research Article
Pages 298-301
Larry W Daniel, Lynn King, Martha Kennedy

[43] Detection of endotoxin by enhancement with toxic shock syndrome toxin-1 (TSST-1) Original Research Article
Pages 302-306
G.A Bohach, P.M Schlievert

[44] Ileal loop fluid accumulation test for diarrheal toxins Original Research Article
Pages 306-323
Merlin S Bergdoll

[45] Monkey feeding test for staphylococcal enterotoxin Original Research Article
Pages 324-333
Merlin S Bergdoll

[46] Assays for epidermolytic toxin of Staphylococcus aureus Original Research Article
Pages 333-338
Joyce C.S de Azavedo, John P Arbuthnott

[47] Immunochemical assays for toxic shock syndrome toxin-1 Original Research Article
Pages 339-344
Patrick M Schlievert

[48] Chick embryo assay for staphylococcal toxic shock syndrome toxin-1 Original Research Article
Pages 344-347
Joyce C.S de Azavedo, Roger N Lucken, John P Arbuthnott

[49] Photoreactive lipids for the study of membrane-penetrating toxins Original Research Article
Pages 347-357
Cesare Montecucco

[50] Electron microscopy: Assays involving negative staining Original Research Article
Pages 357-374
JГёrgen Tranum-Jensen

[51] Electron microscopy: Assays involving freeze-fracture and freeze-etching Original Research Article
Pages 374-389
JГёrgen Tranum-Jensen

[52] Two-dimensional crystals of tetanus toxin Original Research Article
Pages 389-396
J. Reidler, J.P. Robinson

Addendum to article [20]
Page 399
G.A. Bohach, S.J. Cavalieri, I.S. Snyder

Addendum to article [22]
Pages 399-401
Gerald T. Keusch, Arthur Donohue-Rolfe, Mary Jacewicz, Anne V. Kane

Author index
Pages 403-421

Subject index
Pages 423-440