Microbial Natural Products Chemistry: A Metabolomics Approach (Advances in Experimental Medicine and Biology, 1439)

Preface
Contents
Chapter 1: Unveiling Chemical Interactions Between Plants and Fungi Using Metabolomics Approaches
	1.1 Introduction
	1.2 MS-Based Metabolomics
	1.3 NMR-Based Metabolomics
	1.4 Biomarkers Search in Plant-Microorganism Interactions
	1.5 Disease Diagnosis in Plants Using Metabolomics Approaches
	1.6 Chemotaxonomy
	1.7 Signaling Compounds and Plant Defense
	1.8 Plant Resistance
	1.9 Conclusions
	References
Chapter 2: Metabolomics Applied to Cyanobacterial Toxins and Natural Products
	2.1 Introduction
	2.2 Analysis of Cyanotoxins Using Metabolomics Approaches
	2.3 Discovery of Cyanobacterial Natural Products Using Metabolomics Approaches
	2.4 Conclusion and Perspectives
	References
Chapter 3: Unveiling Microbial Chemical Interactions Based on Metabolomics Approaches
	3.1 Introduction
	3.2 Genome Derived Approaches (Genomic)
	3.3 Chemical Diversity Investigation (Phenomics)
	3.4 Metabolomics
		3.4.1 Gas Chromatography – Mass Spectrometer (GC-MS)
		3.4.2 Liquid Chromatography – Mass Spectrometer (LC-MS)
		3.4.3 Direct Analysis of Biological Samples (DI, MALDI and DESI)
	3.5 Perspectives
	References
Chapter 4: Chemical-Biology and Metabolomics Studies in Phage-Host Interactions
	4.1 Introduction
	4.2 Viruses, Bacteria, and Their Interactions
		4.2.1 Mechanisms of Attack and Defense in the Virus-Bacteria System
			4.2.1.1 Bacterial Defense Strategies Mediated by Macromolecules
			4.2.1.2 Protein-Based Phage Parasitism Strategy
		4.2.2 Phage Proteins in the Control of Bacterial Pathogens
		4.2.3 Toxins and Other Metabolites Associated with Virus-Bacteria Interaction
	4.3 Metabolomics and Phage-Bacteria Interaction
		4.3.1 Workflow Metabolomics in Phage-Host Interaction
		4.3.2 Metabolomics Studies in Phage-Host Interaction
	4.4 Final Considerations and Perspectives
	References
Chapter 5: Advances in Mass Spectrometry-Metabolomics Based Approaches
	5.1 Introduction
	5.2 Mass Spectrometry-Based Metabolomics for Bioactive Microbial Compounds Discovery
	5.3 Spatial Mapping of Natural Products by Mass Spectrometry Imaging
	5.4 Ion Mobility Spectrometry (IMS) and Other Ambient Ionization Techniques Applied to Metabolomic Profiles of Microbial Natural Products
	5.5 Dereplication of Microbial Natural Products Through MS
	5.6 Concluding Remarks
	References
Chapter 6: Advances in Microbial NMR Metabolomics
	6.1 NMR Fundamentals
	6.2 Complementary Aspects of NMR to MS
	6.3 Practical Aspects
	6.4 Sample Preparation
	6.5 Data Acquisition
	6.6 Data Processing
	6.7 Statistical Assessment
	6.8 Tools for NMR-Based Metabolomics
	6.9 Applications
	6.10 Conclusion and Future Perspectives
	References
Chapter 7: Sample Preparation in Microbial Metabolomics: Advances and Challenges
	7.1 Introduction
	7.2 Metabolomics Sample Preparation Workflow
		7.2.1 Experimental Design and Pre-Analytical Factors
			7.2.1.1 Cultivation Conditions
			7.2.1.2 Storage and Stability
			7.2.1.3 Sample Harvesting
			7.2.1.4 Quenching
		7.2.2 Sample Extraction
			7.2.2.1 Microbial Intracellular Extraction
			7.2.2.2 Microbial Extracellular Extraction
			7.2.2.3 SPE and SPME
			7.2.2.4 Derivatization
	7.3 Metabolomics Analysis and Sample Normalization
	7.4 Advances in Microbial Sample Preparation
	7.5 Conclusions
	References
Chapter 8: Discovering New Natural Products Using Metabolomics-Based Approaches
	8.1 Introduction
	8.2 Metabolomics and Integrated Analytical Techniques
	8.3 Processes and Concepts: Dereplication and Annotation of Compounds
	8.4 Strategic Tools for Mining Microbial Natural Products
		8.4.1 Databases and Data Analysis Platforms
		8.4.2 Automated Data Processing and In Silico Computational Tools
			8.4.2.1 Examples in Mass Spectrometry
			8.4.2.2 Examples in Nuclear Magnetic Resonance
		8.4.3 Multivariate Statistical Analyses
	8.5 Conclusion
	References
Chapter 9: Microbial Metabolites Annotation by Mass Spectrometry-Based Metabolomics
	9.1 Introduction
	9.2 Mass Spectrometry to Detect Microbial Metabolites
	9.3 Spectral Library Search for Microbial Metabolites Annotation
	9.4 Using In Silico Tools to Improve Annotations
	9.5 Annotation of Substructures
	9.6 How to Validate a Metabolite Annotation
	9.7 Perspectives
	References
Index