Microbial Carotenoids: Methods and Protocols

Front Matter ....Pages i-xii
 Advancement of Biotechnology by Genetic Modifications  (Arnold L. Demain, Sergio Sánchez)....Pages 1-43
 Carotenoids Production: A Healthy and Profitable Industry  (Carlos Barreiro, José-Luis Barredo)....Pages 45-55
 Carotenoids: From Plants to Food and Feed Industries  (Panagiota Langi, Sotirios Kiokias, Theodoros Varzakas, Charalampos Proestos)....Pages 57-71
 Express Analysis of Microalgal Secondary Carotenoids by TLC and UV-Vis Spectroscopy  (Galina S. Minyuk, Alexei E. Solovchenko)....Pages 73-95
 Batch Cultivation for Astaxanthin Analysis Using the Green Microalga Chlorella zofingiensis Under Multitrophic Growth Conditions  (Jin Liu)....Pages 97-106
 Preparative Recovery of Carotenoids from Microalgal Biomass  (María del Carmen Cerón García, Cynthia Victoria González López, José María Fernández Sevilla, Emilio Molina Grima)....Pages 107-115
 Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae  (Yixi Su, Zhiqian Yi, Snædís Huld Björnsdóttir, Sigurdur Brynjolfsson, Weiqi Fu)....Pages 117-126
 Carotenoid Production by Recombinant Corynebacterium glutamicum: Strain Construction, Cultivation, Extraction, and Quantification of Carotenoids and Terpenes  (Nadja A. Henke, Jonas Frohwitter, Petra Peters-Wendisch, Volker F. Wendisch)....Pages 127-141
 Rapid and Selective Screening Method for Isolation and Identification of Carotenoid-Producing Bacteria  (Dalal Asker, Tarek S. Awad, Teruhiko Beppu, Kenji Ueda)....Pages 143-170
 Purification and Identification of Astaxanthin and Its Novel Derivative Produced by Radio-tolerant Sphingomonas astaxanthinifaciens  (Dalal Asker, Tarek S. Awad, Teruhiko Beppu, Kenji Ueda)....Pages 171-192
 Screening, Isolation, and Identification of Zeaxanthin-Producing Bacteria  (Dalal Asker, Tarek S. Awad, Teruhiko Beppu, Kenji Ueda)....Pages 193-209
 Synthesis of Carotenoids of Industrial Interest in the Photosynthetic Bacterium Rhodopseudomonas palustris : Bioengineering and Growth Conditions  (Eric Giraud, Laure Hannibal, Clémence Chaintreuil, Joël Fardoux, André Verméglio)....Pages 211-220
 Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides  (Francisco E. Nicolás, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Sergio López-García, Eusebio Navarro, Santiago Torres-Martínez et al.)....Pages 221-237
 Expression Vectors and Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides  (Enrique A. Iturriaga, María Isabel Alvarez, Arturo P. Eslava, Tamás Papp)....Pages 239-256
 Lycopene Production by Mated Fermentation of Blakeslea trispora  (Sonia Martínez-Cámara, Sara Rubio, Hannah del Río, Marta Rodríguez-Sáiz, José-Luis Barredo)....Pages 257-268
 HPLC Analysis of Carotenoids in Neurosporaxanthin-Producing Fungi  (Dámaso Hornero-Méndez, M. Carmen Limón, Javier Avalos)....Pages 269-281
 Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous  (Carlos García-Estrada, Katarina Kosalková, Isabel-Clara Sánchez-Orejas)....Pages 283-295
 Isolation and Selection of New Astaxanthin-Producing Strains of Phaffia rhodozyma  (Diego Libkind, Martín Moliné, Fernando Colabella)....Pages 297-310
 Engineering Pichia pastoris for the Production of Carotenoids  (Patricia Veiga-Crespo, José Miguel Araya-Garay, Tomás G. Villa)....Pages 311-326
 Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements from Carotenogenic Yeasts  (Marcelo Baeza, María Fernández-Lobato, Jennifer Alcaíno, Víctor Cifuentes)....Pages 327-339
Back Matter ....Pages 341-343