Methods Used in Adenosine Research

Front Matter....Pages i-xvi
Front Matter....Pages 1-1
Synthesis of Adenosine and Adenine Nucleotide Analogs....Pages 3-20
The Measurement of Adenosine and Adenine Nucleotides in Tissues and Body Fluids....Pages 21-42
The Demonstration and Measurement of Adenosine Triphosphate Release from Nerves....Pages 43-63
Front Matter....Pages 65-65
The Study of Adenosine Metabolism in Isolated Cells and Tissues....Pages 67-82
Ectonucleotidases....Pages 83-107
Adenosine Deaminase....Pages 109-125
 S -Adenosylhomocysteine Hydrolase....Pages 127-145
Purine Nucleoside Phosphorylase....Pages 147-162
Front Matter....Pages 163-163
Measurement and Inhibition of Membrane Transport of Adenosine....Pages 165-180
The Use of Ligands in the Study of the Nucleoside-Transport Complex....Pages 181-190
Front Matter....Pages 191-191
The Classification of Receptors for Adenosine and Adenine Nucleotides....Pages 193-212
The Pharmacologic Estimation of Potencies of Agonists and Antagonists in the Classification of Adenosine Receptors....Pages 213-237
Use of Radioligands in the Identification, Classification, and Study of Adenosine Receptors....Pages 239-278
Use of Photoaffinity Labels as P 2 -Purinoceptor Antagonists....Pages 279-292
Use of Structure-Activity Relationships in the Study of Adenosine Receptors....Pages 293-304
Classification of Adenosine Receptors in the Central Nervous System....Pages 305-316
Classification of Adenosine Receptors in Peripheral Tissues....Pages 317-327
Front Matter....Pages 329-329
Criteria for the Involvement of Adenosine in the Regulation of Blood Flow....Pages 331-336
Methods Used to Study the Involvement of Adenosine in the Regulation of Lipolysis....Pages 337-357
Criteria for the Involvement of Adenosine and Adenine Nucleotides in Nonadrenergic, Noncholinergic Transmission....Pages 359-379
Back Matter....Pages 381-384