Methods in Protein Sequence Analysis · 1986

Front Matter....Pages i-xxxv
Front Matter....Pages 1-1
New Ways to Look at Old Proteins....Pages 3-19
The Development of a Facility to Analyze and Synthesize Genes and Proteins....Pages 21-41
Front Matter....Pages 43-43
The Interaction between Molecular Biology and Protein Chemistry....Pages 45-51
The Synergism of Protein Chemistry and Recombinant DNA Techniques....Pages 53-66
The Evolution of the Regulatory Response to Products of Recombinant DNA Technology....Pages 67-78
Analysis of Recombinant Proteins — Current Trends and Practical Limits in Analytical Stringency....Pages 79-95
Methods for Protein Mutagenesis Using Molecular Biology....Pages 97-119
Front Matter....Pages 121-121
The Growing Role of Mass Spectrometry....Pages 123-141
Application of 252 Cf-Plasma Desorption Mass Spectrometry in Protein Primary Structure Determination....Pages 143-147
Protein Sequencing by Tandem Mass Spectrometry....Pages 149-153
Front Matter....Pages 155-155
Strategies for the Analysis of the Structure and Function of Cell Surface Receptors....Pages 157-169
Human von Willebrand Factor and the Problems in Sequence Analysis of a 280 K Dalton Protein....Pages 171-187
Covalent Chromatography, A Powerful Tool in Sequence Studies of Membrane Proteins....Pages 189-209
Front Matter....Pages 211-211
The Role of Microbore HPLC in the Purification of Subnanomole Amounts of Polypeptides and Proteins for Gas-Phase Sequence Analysis....Pages 213-228
Amino Acid Analysis of Submicrogram Hydrolyzate Samples....Pages 229-245
Automated Solid Phase-Sequencing Using Fluorescent Edman Reagents....Pages 247-260
Protein-Blotting from SDS-Polyacrylamide Gels on Glass-Fiber Sheets Coated with Quaternized Ammonium Polybases....Pages 261-275
Sequence Analysis of Proteins from Whole-Cell Lysates After Separation in Analytical Two-Dimensional Gels....Pages 277-294
Sequencing Proteins from Two-Dimensional Gels Using a Liquid Pressure Extraction Technique....Pages 295-302
An Improved Method for Electroblotting of Protein from SDS-PAGE Gels for Direct Microsequencing....Pages 303-309
Front Matter....Pages 211-211
Direct Sequence Analysis of Proteins after Electroblotting from SDS/Polyacrylamide Gel Separations....Pages 311-316
Research Resource Facility Satellite Meeting....Pages 317-319
Front Matter....Pages 321-321
Microheterogeneity Problems in Protein Sequence Analysis....Pages 323-333
Generation of Starting Points for Microsequencing with a Protease Specific for the Amino Side of Aspartyl Residues....Pages 335-337
Proteolytic Cleavage in RP-HPLC Solvents....Pages 339-344
Rapid Generation of Peptides for N-Terminal Sequence Analysis Using Immobilized Pronase....Pages 345-350
C-Terminal Sequence of Proteins: Rapid Isolation and Edman Sequencing of C-Terminal Peptides from Digests....Pages 351-358
Carboxy Terminal Sequence Determination of Peptides and Proteins....Pages 359-364
Front Matter....Pages 365-365
Automated Amino Acid Sequence Assignment: Development of a Fully Automated Protein Sequencer Using Edman Degradation....Pages 367-384
Solid-Phase Microsequencing in Packed Porous Glass Columns: Strategies and Potential for Practical Sequence Analysis....Pages 385-402
Application of Covalently Modified Glass Fibre Supports to Microsequence Analysis....Pages 403-407
Multi Purpose Berlin Sequencer Unit Construction of a Multiple Purpose Machine Assembled as Liquid-, Solid-, or Gas-Phase Sequencer....Pages 409-414
Programmed Cleavage at Proline: Application to the Sequence Determination of Wheat Storage Proteins....Pages 415-420
Front Matter....Pages 421-421
Structural Analysis of Glycoproteins: Identification of Carbohydrates and Other Posttranslational Modifications by Mass Spectrometry....Pages 423-436
Protein Sequence Analysis of Highly-Glycosylated Proteins: Carcinoembryonic Antigen (CEA) as a Model System....Pages 437-449
Identification of Iodinated Amino Acids in Peptide Sequences of Thyroglobulin....Pages 451-456
Methods of Separating, Detecting, Hydrolyzing and Storing Fluorotyrosine, Mono-Iodotyrosine and Di-Iodotyrosine....Pages 457-462
The Determination of Phosphorylation Sites in Peptides and Proteins by Mass Spectrometry....Pages 463-478
Identification of Phosphorylated Amino Acid Residues During Gas Phase Sequencing....Pages 479-482
Mass Spectrometric Characterization of Microscale Enzyme Catalyzed Reactions of Surface-Bound Peptides and Proteins....Pages 483-492
Front Matter....Pages 421-421
Identification of Posttranslational Modifications in Neuropeptides....Pages 493-503
Reinventing the Wheel: General Approaches to the Elucidation of Blocked N-Terminal Sequences....Pages 505-512
The Use of Site-Directed Mutagenesis to Study Disulphide Bond Formation and Posttranslational Processing of Insulin Precursors Expressed and Secreted from Yeast....Pages 513-518
Front Matter....Pages 519-519
Sensitivity and Selectivity in Protein Sequence Comparison....Pages 521-535
Structure and Activity-Regulation of Chimeric Proteins a Case Study of Calcium-Activated Neutral Protease....Pages 537-549
The Receptor-Binding Sequence of Urokinase....Pages 551-554
Identification and Interpretation of Potential Domains in Protein Sequences....Pages 555-563
Strategies for Structural Analysis of Large Proteins: The Utility of Domains, Peptide Sequences, and Peptide Mapping....Pages 565-570
Front Matter....Pages 571-571
Hydrophobicity Profiles Revisited....Pages 573-586
Determination of Secondary and Tertiary Structures of Proteins in Solution by Two-Dimensional NMR....Pages 587-599
Improved Hydrophilicity Plotting Method for Membrane Proteins....Pages 601-607
The Structural Characterization of Protein-Protein Crosslinks in Ribosomes of Different Organisms....Pages 609-614
Carboxyl Group Reactivity in Actin....Pages 615-623
Interactions of Hydroxyboron Compounds with Amino Acids and Proteins....Pages 625-631
Front Matter....Pages 633-633
Concluding Remarks....Pages 635-641
Back Matter....Pages 643-658