Methods in Mycoplasmology. Mycoplasma Characterization

Content: 
Contributors, Page ii
Front Matter, Page iii
Copyright, Page iv
Contributors, Pages xiii-xv
Preface, Pages xvii-xviii, Shmuel Razin, Joseph G. Tully
Contents of Volume II: Diagnostic Mycoplasmology, Pages xix-xxii
A1 - CHARACTERISTICS OF THE MYCOPLASMAS AS A GROUP, Pages 3-7, Shmuel Razin
A2 - PRINCIPLES OF MYCOPLASMA CLASSIFICATION AND TAXONOMY, Pages 9-13, E.A. Freundt
A3 - MYCOPLASMA–ANIMAL HOST INTERRELATIONSHIPS, Pages 15-19, Wallace A. Clyde Jr.
A4 - MYCOPLASMA–PLANT–INSECT INTERRELATIONSHIPS, Pages 21-25, R.F. Whitcomb, J.M. Bové
B1 - INTRODUCTORY COMMENTS, Pages 29-30, Shmuel Razin
B2 - PHASE-CONTRAST MICROSCOPY, Pages 31-33, W. Bredt
B3 - DARK-FIELD MICROSCOPY, Pages 35-37, Joseph G. Tully
B4 - GRAM STAINING TECHNIQUE, Pages 39-41, Michael F. Barile
B5 - TRANSMISSION ELECTRON MICROSCOPY: BASIC TECHNIQUES, Pages 43-50, R.M. Cole
B6 - SCANNING ELECTRON MICROSCOPY OF MYCOPLASMAS, Pages 51-55, Johnny L. Carson, Albert M. Collier
B7 - SPECIALIZED ELECTRON MICROSCOPIC TECHNIQUES FOR MYCOPLASMAS IN ANIMAL TISSUES, Pages 57-61, Johnny L. Carson, Albert M. Collier
B8 - SPECIALIZED ELECTRON MICROSCOPIC TECHNIQUES FOR MYCOPLASMA-LIKE ORGANISMS IN PLANT TISSUES, Pages 63-69, Ronelle C. Norris, Randolph E. McCoy
B9 - SPECIALIZED ELECTRON MICROSCOPIC TECHNIQUES FOR SPIROPLASMAS IN PLANT AND INSECT TISSUES, Pages 71-76, David L. Williamson
B10 - TRANSFER OF SPIROPLASMAS ONTO COLLODION MEMBRANES FOR ELECTRON MICROSCOPIC STUDIES, Pages 77-81, Monique Gamier, J.M. Bové
B11 - IDENTIFICATION OF MYCOPLASMA COLONIES, Pages 83-88, Shmuel Razin
B12 - EXAMINATION OF MYCOPLASMAS FOR MOTILITY, Pages 89-90, W. Bredt
C1 - INTRODUCTORY REMARKS, Pages 93-97, A.W. Rodwell
C2 - GENERAL CULTIVATION TECHNIQUES FOR MYCOPLASMAS AND SPIROPLASMAS, Pages 99-101, Joseph G. Tully
C3 - COLLECTION AND TRANSPORT OF SPECIMENS, Pages 103-107, Wallace A. Clyde Jr., William M. McCormack
C4 - GROWTH INHIBITORY FACTORS IN ANIMAL AND PLANT TISSUES, Pages 109-114, David Taylor-Robinson, Tseh An Chen
C5 - PRECAUTIONS IN HANDLING INFECTIOUS MATERIAL, Pages 115-119, Gerard J. McGarrity
C6 - STERILITY AND QUALITY CONTROL OF MYCOPLASMA CULTURE MEDIA, Pages 121-125, Joseph G. Tully, David L. Rose
C7 - CULTURE MEDIA FOR CLASSIC MYCOPLASMAS, Pages 127-135, E.A. Freundt
C8 - CULTURE MEDIA FOR UREAPLASMAS, Pages 137-146, Maurice C. Shepard
C9 - CULTURE MEDIA FOR SPIROPLASMAS, Pages 147-158, Robert F. Whitcomb
C10 - CULTURE MEDIA FOR ANAEROPLASMAS, Pages 159-162, Isadore M. Robinson
C11 - DEFINED AND PARTLY DEFINED MEDIA, Pages 163-172, A.W. Rodwell
C12 - CLONING AND FILTRATION TECHNIQUES FOR MYCOPLASMAS, Pages 173-177, Joseph G. Tully
C13 - LABELING OF CELLULAR COMPONENTS DURING GROWTH, Pages 179-184, Shlomo Rottem
C14 - METHODS FOR DIRECT AND INDIRECT MEASUREMENT OF MYCOPLASMA GROWTH, Pages 185-196, A.W. Rodwell, R.F. Whitcomb
C15 - PRESERVATION OF MYCOPLASMA CULTURES AND CULTURE COLLECTIONS, Pages 197-204, R.H. Leach
C16 - BACTERIAL AND FUNGAL INHIBITORS IN MYCOPLASMA CULTURE MEDIA, Pages 205-209, Joseph G. Tully
C17 - OPTIMAL TEMPERATURE AND ATMOSPHERIC CONDITIONS FOR GROWTH, Pages 211-215, Roberta S. Gardella, Richard A. DelGiudice
D1 - INTRODUCTORY REMARKS, Pages 219-220, Shmuel Razin
D2 - HARVEST AND WASHING OF MYCOPLASMAS, Pages 221-223, Shlomo Rottem
D3 - CELL LYSIS AND ISOLATION OF MEMBRANES, Pages 225-233, Shmuel Razin
D4 - MANIPULATION OF MEMBRANE LIPID COMPOSITION, Pages 235-239, Ronald N. McElhaney
D5 - ELECTROPHORETIC CHARACTERIZATION OF MYCOPLASMA PROTEINS, Pages 241-255, C. Mouches, J.M. Bové
D6 - CHARACTERIZATION OF MEMBRANE PROTEINS BY CROSSED IMMUNOELECTROPHORESIS, Pages 257-267, Karl-Erik Johansson, Henri Wróblewski
D7 - CHARACTERIZATION OF MEMBRANE LIPIDS, Pages 269-275, Shlomo Rottem
D8 - CHARACTERIZATION OF MEMBRANE LIPOGLYCANS, Pages 277-283, Paul F. Smith, Thomas A. Langworthy
D9 - CHARACTERIZATION OF CARBOHYDRATE COMPONENTS OF MYCOPLASMA MEMBRANES, Pages 285-294, Itzhak Kahane, Hans-Gerd Schiefer
D10 - DNA EXTRACTION AND PURIFICATION, Pages 295-299, Patricia Carle, Colette Saillard, J.M. Bové
D11 - DETERMINATION OF GUANINE PLUS CYTOSINE CONTENT OF DNA, Pages 301-308, Patricia Carle, Colette Saillard, J.M. Bové
D12 - GENOME SIZE DETERMINATION, Pages 309-311, Patricia Carle, J.M. Bové
D13 - EcoRI RESTRICTION ENZYME ANALYSIS OF MYCOPLASMA DNA, Pages 313-318, Colette Saillard, J.M. Bové
D14 - HYBRIDIZATION BETWEEN MYCOPLASMA DNAs, Pages 319-325, J.R. Degorce-Dumas, Berenice Ricard, J.M. Bové
D15 - LOCALIZATION OF ENZYMES IN MYCOPLASMAS: PREPARATORY STEPS, Pages 327-332, J.Dennis Pollack
E1 - INTRODUCTORY REMARKS, Pages 335-336, Shmuel Razin
E2 - SUGAR FERMENTATION, Pages 337-343, Shmuel Razin, Vincent P. Cirillo
E3 - ARGININE HYDROLYSIS, Pages 345-349, Michael F. Barile
E4 - UREA HYDROLYSIS, Pages 351-353, Shmuel Razin
E5 - TESTS FOR DIGITONIN SENSITIVITY AND STEROL REQUIREMENT, Pages 355-362, Joseph G. Tully
E6 - PHOSPHATASE ACTIVITY, Pages 363-366, Janet M. Bradbury
E7 - PROTEOLYTIC ACTIVITY, Pages 367-371, E.A. Freundt
E8 - FILM AND SPOT PRODUCTION, Pages 373-374, E.A. Freundt
E9 - PIGMENTED CAROTENOIDS, Pages 375-376, Shmuel Razin
E10 - TETRAZOLIUM REDUCTION, Pages 377-378, Laurence B. Senterfit
E11 - HEMAGGLUTINATION, HEMADSORPTION, AND HEMOLYSIS, Pages 379-384, Roberta S. Gardella, Richard A. DelGiudice
E12 - DETECTION OF β-D-GLUCOSIDASE: HYDROLYSIS OF ESCULIN AND ARBUTIN, Pages 385-389, David L. Rose, Joseph G. Tully
E13 - ISOZYME RESOLUTION IN MYCOPLASMAS, Pages 391-396, Stephen J. O'Brien, Michael F. Barile
F1 - INTRODUCTORY REMARKS, Pages 399-400, Joseph G. Tully
F2 - PREPARATION OF ANTIGENS AND ANTISERA, Pages 401-404, Laurence B. Senterfit
F3 - GROWTH INHIBITION TESTS, Pages 405-410, Wallace A. Clyde Jr.
F4 - METABOLISM INHIBITION TESTS, Pages 411-417, David Taylor-Robinson
F5 - TETRAZOLIUM REDUCTION INHIBITION, Pages 419-421, Laurence B. Senterfit
F6 - THE MYCOPLASMACIDAL TEST (MCT), Pages 423-430, Helmut Brunner
F7 - IMMUNOFLUORESCENCE, Pages 431-439, Roberta S. Gardella, Richard A. DelGiudice, Joseph G. Tully
F8 - AGAR PRECIPITIN AND IMMUNOELECTROPHORETIC METHODS FOR DETECTION OF MYCOPLASMIC ANTIGENS, Pages 441-456, George E. Kenny
F9 - ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETECTION OF ANTI-MYCOPLASMAL ANTIBODY, Pages 457-469, Gail H. Cassell, Mary B. Brown
F10 - APPLICATION OF ELISA TO SPIROPLASMA DETECTION AND CLASSIFICATION, Pages 471-476, Colette Saillard, J.M. Bové
F11 - THE COMBINED DEFORMATION–METABOLISM INHIBITION TEST, Pages 477-483, David L Williamson
F12 - SINGLE RADIAL HEMOLYSIS TECHNIQUE, Pages 485-487, Christopher J. Howard
F13 - GROWTH PRECIPITATION TEST, Pages 489-492, Henning Ernø, Kirsten Peterslund
Appendix Tables 1–4, Pages 494-500
Index, Pages 501-504