Methods in DNA Amplification

Front Matter....Pages I-IX
Front Matter....Pages 1-1
The Polymerase Chain Reaction and Fixed Tissues....Pages 3-9
Impact of PCR on the Pathologist’s World....Pages 11-17
Sensitive and Rapid Detection and Quantification of Nucleic Acids....Pages 19-26
Polymorphic Keratins as Detected by PCR and SSCP....Pages 27-35
Production of Antibodies of Monoclonal Specificity Without the Use of Hybridoma Cell Lines....Pages 37-46
Use of Chelex 100 TM in the Extraction of Viruses from Diverse Cell-Free Clinical Samples for PCR....Pages 47-53
Detection of Amplified Oncogenes by Differential Polymerase Chain Reaction....Pages 55-64
Resolution of a Secondary Structure in an Unknown mRNA 5’ End....Pages 65-69
Automated Solid-Phase Sequencing of Genomic DNA Obtained from Polymerase Chain Reaction....Pages 71-79
Front Matter....Pages 81-81
Ligase-Mediated Detection Techniques....Pages 83-92
Qualitative and Quantitative Detection of Nucleic Acids of Infectious Agents by NASBA....Pages 93-102
Application of NASBA to the Detection of Listeria Monocytogenes ....Pages 103-110
Front Matter....Pages 111-111
Confirmation of Hepatitis C Virus Carrier State by Polymerase Chain Reaction in Cases with Nondiagnostic Serologies....Pages 113-121
Confirmation of Hepatitis C Virus Positive Seras by Polymerase Chain Reaction....Pages 123-126
Specific Detection and Rapid Identification of Human Enteroviruses by PCR Amplification....Pages 127-134
Detection of Herpes Simplex Virus Using Polymerase Chain Reaction....Pages 135-143
Detection of Human Cytomegalovirus (HCMV) — DNA from Granulocytes and Peripheral Blood Mononuclear Cells (PBMNC) of Immunosuppressed Patients by Nested PCR: Lack of Correlation to Isolation of Infectious Virus....Pages 145-153
Timing of Mother-to Child Transmission of HIV-1 as Determinated by Nested Polymerase Chain Reaction — A Cohort Study in Kigali, Rwanda....Pages 155-161
One-Day Detection of Enteroviruses in Clinical Specimens by Magnetic Bead Extraction of Viral RNA and Nested Polymerase Chain Reaction....Pages 163-168
Monitoring of Poliovirus Strains Circulating in the Environment and Their Intertypic and Intratypic Differentiation by Rflp Analysis of a PCR Product Derived from the 5’ Non-Coding Region....Pages 169-178
Front Matter....Pages 111-111
Detection of Adenoviruses in Blood by Polymerase Chain Reaction....Pages 179-184
Detection of the HPV 16 E2 Gene in Nonkeratinizing Squamous Cervical Carcinomas by PCR....Pages 185-189
Front Matter....Pages 191-191
Identification of Toxoplasma-DNA by Polymerase Chain Reaction in Peripheral Blood Leukocytes of Patients with Suspected Toxoplasmosis....Pages 193-196
Detection of Mycobacterium Tuberculosis in Clinical Samples by Polymerase Chain Reaction....Pages 197-203
Tuberculosis in HIV Infected Patients Detected by PCR: A Comparison with Clinical Data....Pages 205-210
Detection of Borrelia Burgdorferi in Human Skin Biopsies by a Nested Polymerase Chain Reaction....Pages 211-217
The Application of PCR Fingerprinting to the Epidemiologic Analysis of Bacterial and Fungal Pathogens....Pages 219-225
Back Matter....Pages 227-251