Methods in Cell Biology, Vol. 9

Content: 
Edited by
Page iii

Copyright page
Page iv

List Of Contributors
Pages xi-xii

Preface
Page xiii
David M. Prescoit

Contents Of Previous Volumes
Pages xv-xxv

Chapter 1 Preparation of Large Quantities of Pure Bovine Lymphocytes and a Monolayer Technique for Lymphocyte Cultivation Original Research Article
Pages 1-11
J. Hinrich Peters

Chapter 2 Methods to Culture Diploid Fibroblasts on a Large Scale Original Research Article
Pages 13-23
H.W. Rüdiger

Chapter 3 Partition of Cells in Two-Polymer Aqueous Phases: A Method for Separating Cells and for Obtaining Information on Their Surface Properties Original Research Article
Pages 25-50
Harry Walter

Chapter 4 Synchronization of Cell Division in Vivo through the Combined Use of CytosineArabinoside and Colcemid Original Research Article
Pages 51-69
Robert S. Verbin, Emmanuel Farber

Chapter 5 The Accumulation and Selective Detachment of Mitotic Cells Original Research Article
Pages 71-84
Edwin V. Gaffney

Chapter 6 Use of the Mitotic Selection Procedure for Cell Cycle Analysis: Emphasis on Radiation-Induced Mitotic Delay Original Research Article
Pages 85-101
D.P. Highfield, W.C. Dewey

Chapter 7 Evaluation of S Phase Synchronization by Analysis of DNA Replication in 5-Bromodeoxyuridine Original Research Article
Pages 103-113
Raymond E. Meyn, Roger R. Hewitt, Ronald M. Humphrey

Chapter 8 Application of Precursors Adsorbed on Activated Charcoal for Labeling of Mammalian DNA in Vivo Original Research Article
Pages 115-122
George Russev, Roumen Tsanev

Chapter 9 Growth of Functional Glial Cells in a Serumless Medium Original Research Article
Pages 123-137
Sam T. Donta

Chapter 10 Miniature Tissue Culture Technique with a Modified (Bottomless) Plastic Microplate Original Research Article
Pages 139-155
Eliseo Manuel Hernández-Baumgarten

Chapter 11 Agar Plate Culture and Lederberg-Style Replica Plating of Mammalian Cells Original Research Article
Pages 157-178
Toshio Kuroki

Chapter 12 Methods and Applications of Flow Systems for Analysis and Sorting of Mammalian Cells Original Research Article
Pages 179-246
H.A. Crissman, P.F. Mullaney, J.A. Steinkamp

Chapter 13 Purification of Surface Membranes from Rat Brain Cells Original Research Article
Pages 247-257
Kari Hemminki

Chapter 14 The Plasma Membrane of KB Cells; Isolation and Properties Original Research Article
Pages 259-280
F.C. Charalampous, N.K. Gonatas

Chapter 15 The Isolation of Nuclei from Paramecium aurelia Original Research Article
Pages 281-309
Donald J. Cummings, Andrew Tait

Chapter 16 Isolation of Micro- and Macronuclei of Tetrahymena pyriformis Original Research Article
Pages 311-327
Martin A. Gorovsky, Meng-Chao Yao, Josephine Bowen Keevert, Gloria Lorick Pleger

Chapter 17 Manipulations with Tetrahymena pyriformis on Solid Medium Original Research Article
Pages 329-348
Enore Gardonio, Michael Crerar, Ronald E. Pearlman

Chapter 18 The Isolation of Nuclei with Citric Acid and the Analysis of Proteins by Two-Dimensional Polyacrylamide Gel Electrophoresis Original Research Article
Pages 349-376
Charles W. Taylor, Lynn C. Yeoman, Harris Busch

Chapter 19 Isolation and Manipulation of Salivary Gland Nuclei and Chromosomes Original Research Article
Pages 377-390
M. Robert

Subject Index
Pages 391-397