Methods for Analysis of Golgi Complex Function

Content: 
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xiii-xviii

Preface
Pages xix-xx
David Stephens, Franck Perez

Chapter 1 - Analysis of Golgi Complex Functions: In Vitro Reconstitution Systems
Pages 3-14
Frank Adolf, Felix T. Wieland

Chapter 2 - RNA Interference Approaches to Examine Golgi Function in Animal Cell Culture
Pages 15-34
Victoria J. Miller, Caroline M. McKinnon, Harry Mellor, David J. Stephens

Chapter 3 - Trafficking Along the Secretory Pathway in Drosophila Cell Line and Tissues: A Light and Electron Microscopy Approach
Pages 35-49
Margarita Zacharogianni, Catherine Rabouille

Chapter 4 - Studying Membrane Trafficking in the Worm C. elegans by RNA Interference
Pages 51-68
Zita Balklava, Elizabeth Sztul

Chapter 5 - Endomembrane and Golgi Traffic in Plant Cells
Pages 69-83
Giovanni Stefano, Anne Osterrieder, Chris Hawes, Federica Brandizzi

Chapter 6 - Biochemical Analysis of Secretory Trafficking in Mammalian Cells
Pages 85-103
Peristera Roboti, Tomasz M. Witkos, Martin Lowe

Chapter 7 - Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems
Pages 105-123
Alejo E. Rodríguez-Fraticelli, Fernando Martín-Belmonte

Chapter 8 - Using Replication Defective Viruses to Analyze Membrane Trafficking in Polarized Epithelial Cells
Pages 125-137
Lucy Pigati, Richard S. Kang, Heike Fölsch

Chapter 9 - SNAP-Tagging the Retrograde Route
Pages 139-155
Ludger Johannes, Massiullah Shafaq-Zadah

Chapter 10 - Assessing ER and Golgi N-Glycosylation Process Using Metabolic Labeling in Mammalian Cultured Cells
Pages 157-176
Romain Péanne, Jorick Vanbeselaere, Dorothée Vicogne, Anne-Marie Mir, Christophe Biot, Gert Matthijs, Yann Guérardel, François Foulquier

Chapter 11 - Fluorescence-Based Analysis of Trafficking in Mammalian Cells
Pages 179-194
Gaelle Boncompain, Franck Perez

Chapter 12 - Photobleaching Methods to Study Golgi Complex Dynamics in Living Cells
Pages 195-216
Erik Lee Snapp

Chapter 13 - Photohighlighting Approaches to Access Membrane Dynamics of the Golgi Apparatus
Pages 217-234
Prabuddha Sengupta, Jennifer Lippincott-Schwartz

Chapter 14 - Live Cell Visualization of Golgi Membrane Dynamics by Super-resolution Confocal Live Imaging Microscopy
Pages 235-242
Kazuo Kurokawa, Midori Ishii, Yasuyuki Suda, Akira Ichihara, Akihiko Nakano

Chapter 15 - Analysis of Golgi Complex Function Using Correlative Light-Electron Microscopy
Pages 243-258
Elena V. Polishchuk, Roman S. Polishchuk

Chapter 16 - Viewing Golgi Structure and Function from a Different Perspective—Insights from Electron Tomography
Pages 259-279
Brad J. Marsh, Margit Pavelka

Chapter 17 - High-Content Screening and Analysis of the Golgi Complex
Pages 281-295
George Galea, Jeremy C. Simpson

Chapter 18 - (Re)Modeling the Golgi
Pages 299-310
Pierre Sens, Madan Rao

Chapter 19 - Golgi Depletion from Living Cells with Laser Nanosurgery
Pages 311-324
Paolo Ronchi, Rainer Pepperkok

Chapter 20 - Studying Intracellular Trafficking Pathways with Probabilistic Density Maps
Pages 325-343
Kristine Schauer, Tarn Duong, Carina S. Gomes-Santos, Bruno Goud

Chapter 21 - Methods to Study Signaling at the Golgi Apparatus
Pages 345-358
Veronika Reitere, Francesco Baschieri, Valentina Millarte, Hesso Farhan

Chapter 22 - Golgi-Dependent Signaling: Self-Coordination of Membrane Trafficking
Pages 359-382
Jorge Cancino, Anita Capalbo, Alberto Luini

Chapter 23 - Signaling at the Golgi During Mitosis
Pages 383-400
Antonino Colanzi, Christine Sütterlin

Chapter 24 - Ice Recovery Assay for Detection of Golgi-Derived Microtubules
Pages 401-415
Ashley D. Grimaldi, Maria Fomicheva, Irina Kaverina

Index
Pages 417-431

Volumes in Series
Pages 433-443