Methionine Dependence of Cancer and Aging: Methods and Protocols

Front Matter ....Pages i-xii
 Methods to Study the Role of Methionine-Restricted Diet and Methioninase in Cancer Growth Control  (Shushma Chaturvedi, Joseph R. Bertino)....Pages 1-12
 Altered Methionine Metabolism in Cancer Cells  (Robert M. Hoffman, Peter H. Stern, Dennis W. Coalson, C. Douglas Wallace, Richard W. Erbe)....Pages 13-26
 Linkage of Methionine Dependence and Other Features of Malignancy  (Robert M. Hoffman, Richard W. Erbe)....Pages 27-36
 Isolation and Characterization of Methionine-Independent Clones from Methionine-Dependent Cancer Cells  (Stacey L. Borrego, Da-Wei Lin, Peter Kaiser)....Pages 37-48
 Tumor-Specific S/G2-Phase Cell Cycle Arrest of Cancer Cells by Methionine Restriction  (Robert M. Hoffman, Shuya Yano)....Pages 49-60
 Preclinical Breast Cancer Models to Investigate Metabolic Priming by Methionine Restriction  (Elena Strekalova, Dmitry Malin, Harisha Rajanala, Vincent L. Cryns)....Pages 61-73
 Efficacy of Methionine-Restricted Diets on Cancers In Vivo  (Robert M. Hoffman, Yasunori Hoshiya, Weiyan Guo)....Pages 75-81
 Dietary Methionine Restriction-Based Cancer Chemotherapy in Rodents  (Robert M. Hoffman, Peter H. Stern)....Pages 83-94
 Clinical Studies of Methionine-Restricted Diets for Cancer Patients  (Robert M. Hoffman)....Pages 95-105
 Development of Recombinant Methioninase for Cancer Treatment  (Robert M. Hoffman, Yuying Tan, Shukuan Li, Qinghong Han, Shigeo Yagi, Tomoaki Takakura et al.)....Pages 107-131
 Methioninase Cell-Cycle Trap Cancer Chemotherapy  (Robert M. Hoffman, Shuya Yano, Kentaro Igarashi)....Pages 133-148
 High Efficacy of Recombinant Methioninase on Patient-Derived Orthotopic Xenograft (PDOX) Mouse Models of Cancer  (Robert M. Hoffman, Takashi Murakami, Kei Kawaguchi, Kentaro Igarashi, Yuying Tan, Shukuan Li et al.)....Pages 149-161
 Total Methionine Restriction Treatment of Cancer  (Robert M. Hoffman, Demetrius M. Kokkinakis, Eugene P. Frenkel)....Pages 163-171
 Methioninase Gene Therapy  (Robert M. Hoffman, Kenji Miki, Waddah Al-Refaie, Mingxu Xu, Yuying Tan)....Pages 173-197
 Selenomethionine and Methioninase: Selenium Free Radical Anticancer Activity  (Julian E. Spallholz)....Pages 199-210
 Safety and Toxicity of Recombinant Methioninase and Polyethylene Glycol (PEG) Recombinant Methioninase in Primates  (Robert M. Hoffman, Zhijian Yang, Yuying Tan, Qinghong Han, Shukuan Li, Shigeo Yagi)....Pages 211-229
 Pilot Phase I Clinical Trial of Methioninase on High-Stage Cancer Patients: Rapid Depletion of Circulating Methionine  (Robert M. Hoffman, Yuying Tan, Shukuan Li, Qinghong Han, Jorge Zavala Sr, Jorge Zavala Jr)....Pages 231-242
 Methionine Gamma Lyase from Clostridium sporogenes Increases the Anticancer Efficacy of Doxorubicin on A549 Cancer Cells In Vitro and Human Cancer Xenografts  (V. S. Pokrovsky, N. Yu. Anisimova, D. Zh. Davydov, S. V. Bazhenov, N. V. Bulushova, G. B. Zavilgelsky et al.)....Pages 243-261
 Methionine Restriction and Life-Span Extension  (Robert M. Hoffman)....Pages 263-266
 l-[Methyl-11C] Methionine-Positron-Emission Tomography (MET-PET)  (Robert M. Hoffman)....Pages 267-271
 Is the Hoffman Effect for Methionine Overuse Analogous to the Warburg Effect for Glucose Overuse in Cancer?  (Robert M. Hoffman)....Pages 273-278
 Recombinant Methioninase as a DNA Demethylation Agent  (Robert M. Hoffman, David Machover)....Pages 279-284
 Homocysteine, Thioretinaco Ozonide, and Oxidative Phosphorylation in Cancer and Aging: A Proposed Clinical Trial Protocol  (Kilmer S. McCully)....Pages 285-310
 Afterword: Oral Methioninase—Answer to Cancer and Fountain of Youth?  (Robert M. Hoffman, Qinghong Han, Kei Kawaguchi, Shukuan Li, Yuying Tan)....Pages 311-322
Back Matter ....Pages 323-329