Metabolism and Enzymology of Nucleic Acids: Including Gene Manipulations

Front Matter....Pages i-x
DNA Methylation and DNA Methyltransferase in Wheat....Pages 1-7
The X-Ray Analysis of Ribonuclease Sa....Pages 9-18
The Nature of Distribution of Ribonucleotide Reductases....Pages 19-25
Cloning, Expression and Nucleotide Sequence of a Gene Encoding a Second Thioredoxin from Corynebacterium Nephridii ....Pages 27-35
Thioredoxin from Streptomyces Aureofaciens ....Pages 37-41
Two-Dimensional 1 H-NMR Investigation of Ribonuclease T 1 and the Complexes of Rnase T 1 with 2’- and 3’-Guanosine Monophosphate....Pages 43-53
Nucleoside Phosphotransferase and Nuclease S 1 Two Enzymes with Acylphosphate Intermediates, But Different Mechanisms....Pages 55-61
Probing the Mechanism of Enzyme Action Through Specific Amino Acid Substitutions....Pages 63-63
Site-Specific Restriction Endonuclease Sau BMK I from Streptomyces Aureofaciens ....Pages 65-71
The Effects of N4- and C5-Methylati on of Cytosine on DNA Physical Properties and Restriction Endonuclease Action....Pages 73-78
Regulation of RNA Synthesis in Yeast: The Stringent Control of Ribosomal RNA Synthesis....Pages 79-84
In Vitro Study on Ribosome Degradation in Rat Liver....Pages 85-93
Specific Labelling in the Functional Domain of Elongation Factor EF-Tu from Bacillus Stearothermophilus and from Bacillus Subtilis ....Pages 95-100
GTP Expense at Codon-Specific Translation and During Misreading....Pages 101-108
Site-Directed Mutagenesis of Elongation Factor Tu....Pages 109-116
A Site-Mutated rrsD Gene Expression Construct: Impairment of Ribosomal Function....Pages 117-122
Expression of Prochymosin cDNA in E.Coli ....Pages 123-126
Control of the Cell Cycle Start by Protein Kinase Genes in Saccharomyces Cerevisiae ....Pages 127-129
Molecular Analysis of Mitochondrial DNA and Cloning of its rDNA Fragments from Tomato Cell Suspension Culture....Pages 131-136
Nucleotide Compartmentation During Synthesis of Cytoplasmic rRNA and tRNA as well as Cytoplasmic rRNA and Mitochondrial rRNA in Tomato Cell Suspension Culture....Pages 137-141
Intracellular Location of Some Purine- and Pyrimidine-Metabolizing Enzymes in Plant Cells....Pages 143-148
Some Properties of Dinucleoside Oligophosphate Phosphorylase from Euglena Gracilis ....Pages 149-153
Expression of Genes Coding for Novobiocin Biosynthesis and Novobiocin Resistance in Streptomyces Niveus ....Pages 155-159
Chromosomal Rearrangements Affecting the Regulation of Uridine Phosphorylase Gene Expression in Escherichia Coli ....Pages 161-166
Repeated Sequences in Chromosomal DNA of Streptomyces Aureofaciens 2201....Pages 167-171
Stable Gene Duplication by “Puzzle” Shot Gun Cloning in Inverted Repeats of a Streptococcal Plasmid....Pages 173-173
Different Types of Repetitive Elements in Mammalian Genome: Cloning and Characterization of cDNA Copies of Transcripts Containing Repetitive Sequences....Pages 175-181
Nucleotide Sequence Analysis of the 5′-End Of Chicken c-myb Gene....Pages 183-190
Cloning and Characterization of Human Genes Expressed in Neural Cells....Pages 191-196
Detection of a β-Globin Intron Mutation in a β-Thalas Semic Patient from Azerbaijan....Pages 197-201
The Gene of the Yeast Ribosomal Protein L32....Pages 203-212
Structural Comparison of Some Microbial Ribonucleases....Pages 213-216
Structure Bases for Nucleotide Recognition by Guanyl-Specific Ribonucleases....Pages 217-221
Crystallization and Preliminary X-Ray Structural Studies of RNase Th 1 from Trichoderma Harzianum ....Pages 223-225
Three-Dimensional Structure of RNase C 2 from Aspergillus Clavatus at 1.35 Å Resolution....Pages 227-231
Amino Acid Composition and Influence of the Modification of Some Amino Acid Residues on the Catalytic Activity of the Spleen Exonuclease....Pages 233-236
Binding of the Metal Ions in the Active Site of the Exonuclease from Crotalus Adamanteus Venom....Pages 237-243
Ribosomal Nuclease with Activity Towards Double-Stranded RNA....Pages 245-250
Investigation of Immunoglobulin Light and Heavy Chain Genes Responsible for the Synthesis of Antibodies in Hybridoma PTF.02....Pages 251-257
Regulation of Cytochrome P-450 Biosynthesis in Alkane Assimilating Yeast....Pages 259-265
On the Mechanism of Reutilization of Nuclear RNA Degradation Products: A Study in Vivo ....Pages 267-273
Immunological Characterization of DNA-Dependent RNA Polymerase(s) of Spinach Chloroplasts....Pages 275-281
DNA Crosslinked by UV Light to the Nuclear Lamina is Enriched in Pulse-Labelled Sequences....Pages 283-287
The Mechanism of Arabinofuranosyl-Cytosine Inhibition in Undifferentiated Human B Lymphocytes....Pages 289-296
Closing of the Symposium by J. Zelinka Corresponding Member of the Czechoslovak and Slovak Academy of Sciences, Director of the Institute of Molecular Biology, Slovak Academy of Sciences....Pages 297-297
Back Matter....Pages 299-312