Mass Spectrometry in the Biological Sciences

Front Matter....Pages i-xii
Mass Spectrometry in the Health and Life Sciences....Pages 1-4
Designing Time-of-Flight Mass Spectrometers for the Future: Multichannel Recording of Peptide Amino Acid Sequences....Pages 5-23
The Role of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in Biological Research — New Developments and Applications....Pages 25-68
Electrospray Ionization Mass Spectrometry: The Observation of Covalent, Ionic and Non-Covalent Interactions.....Pages 69-84
Using Deuterium Exchange and Mass Spectrometry to Study Protein Structure....Pages 85-104
MALDI-TOF Mass Spectrometry in the Protein Biochemistry Lab: From Characterization of Cell Cycle Regulators to the Quest for Novel Antibiotics....Pages 105-133
Chemistry, Mass Spectrometry and Peptide-Mass Databases: Evolution of Methods for the Rapid Identification and Mapping of Cellular Proteins....Pages 135-150
Identification of Electroblotted Proteins by Peptide Mass Searching of a Sequence Database....Pages 151-170
Mass Spectrometric Identification of Proteins Isolated By Two-Dimensional Gel Electrophoresis....Pages 171-202
Infrared-Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (IR-MALDI-MS) of Proteins Electroblotted onto Polymer Membranes After SDS-PAGE Separation....Pages 203-216
Picoliter Vials: A New Sample Handling Technique for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry....Pages 217-226
Direct Neuropeptide Profiling of Single Neurons and Target Tissue by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry....Pages 227-243
Approaches to the Practical Use of MS/MS in a Protein Sequencing Facility....Pages 245-265
Capillary Liquid Chromatography/Electrospray Mass Spectrometry with a Magnetic Sector Instrument for Accurate Mass Measurement of Peptide and Protein Mixtures....Pages 267-280
Mass Spectrometric Methods for Peptide Sequencing: Applications to Immunology and Protein Acylation....Pages 281-305
A Multifaceted Strategy for the Characterization of Recombinant gD-2, a Potential Herpes Vaccine....Pages 307-331
Identifying Unexpected Protein Modifications....Pages 333-350
Mass Spectrometry in Carbohydrate Recognition Studies....Pages 351-364
Mass Spectrometric and Tandem Mass Spectrometric Approaches to the Analysis of Glycoconjugates....Pages 365-384
Structural Determination and Biosynthetic Studies of the Rhizobial Nod Metabolites: The Lipo-Chitin Oligosaccharides....Pages 385-401
Mass Spectrometry as a Tool to Determine the Structures and Heterogeneity of Bacterial Lipooligosaccharides (LOS)....Pages 403-424
Applications of Tandem Liquid Chromatography/Mass Spectrometry in Drug Biotransformation and Quantification Studies....Pages 425-449
Isoeicosanoids: Mass Spectrometry of Free Radical Oxidation Products Derived From Arachidonoyl Phospholipids....Pages 451-476
Applications of Accelerator Mass Spectrometry in Toxicology: A Highly Sensitive Tool for Low-Level Isotope Measurements....Pages 477-495
Characterization of Posttranscriptional Modification in Nucleic Acids by Tandem Mass Spectrometry....Pages 497-517
The Future of DNA Sequencing: Methods and Applications....Pages 519-533
Back Matter....Pages 535-570