Mass Spectrometry in Biology & Medicine

Front Matter....Pages i-xiv
A New Delayed Extraction MALDI-TOF MS-MS for Characterization of Protein Digests....Pages 1-16
Measurements of Protein Structure and Noncovalent Interactions by Time-of-Flight Mass Spectrometry with Orthogonal Ion Injection....Pages 17-30
Isotopic Amplification, H/D Exchange, and Other Mass Spectrometric Strategies for Characterization of Biomacromolecular Topology and Binding Sites....Pages 31-52
Probing the Nature of Amyloidogenic Proteins by Mass Spectrometry....Pages 53-72
Studying Noncovalent Small Molecule Interactions with Protein and RNA Targets by Mass Spectrometry....Pages 73-90
Identification of Protein-Protein Interfaces by Amide Proton Exchange Coupled to MALDI-TOF Mass Spectrometry....Pages 91-109
Electron Capture Dissociation Produces Many More Protein Backbone Cleavages Than Collisional and IR Excitation....Pages 111-120
Protein Micro-Characterization by Mass Spectrometry: Sample Handling and Data Flow....Pages 121-142
Towards an Integrated Analytical Technology for the Generation of Multidimensional Protein Expression Maps....Pages 143-162
Sequencing the Primordial Soup....Pages 163-177
Coaxial Nanospray Coupled with a Hybrid Quadrupole/Time-of-Flight Tandem Mass Spectrometer for Proteome Studies....Pages 179-198
Life Without Databases: De Novo Sequencing of Small Gene Products and Complete Characterization of Posttranslational Modifications....Pages 199-215
Investigation of Intact Subunit Polypeptide Composition of the 20S Proteasome Complex from Rat Liver Using Mass Spectrometry....Pages 217-235
Deciphering Functionally Important Multiprotein Complexes by Mass Spectrometry....Pages 237-269
Investigation of Apoptosis-Involved Processes by Mass Spectrometric Identification of the Apoptosis-Associated Proteins in IgM-Induced Burkitt Lymphoma Cells....Pages 271-287
IR-MALDI — Softer Ionization in MALDI-MS for Studies of Labile Macromolecules....Pages 289-307
Identification of in-vivo Phosphorylation Sites with Mass Spectrometry....Pages 309-328
Determination of Enzyme Mechanisms by Stopped-Flow ESI-MS....Pages 329-364
Glycosylation of Proteins — A Major Challenge in Mass Spectrometry and Proteomics....Pages 365-381
Site-specific Characterization of the N-linked Glycans of Murine PrP Sc Using Advanced Methods of Electrospray Mass Spectrometry....Pages 383-402
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of N-Linked Carbohydrates and Related Compounds....Pages 403-437
Electrophoretic and Mass Spectrometric Strategies for the Identification of Lipopolysaccharides and Immunodeterminants in Pathogenic Strains of Haemophilus influenzae ; Application to Clinical Isolates....Pages 439-462
Mycobacterial Lipoglycans: Structure and Roles in Mycobacterial Immunity....Pages 463-480
The Impact of Drug Metabolism in Contemporary Drug Discovery: New Opportunities and Challenges for Mass Spectrometry....Pages 481-496
Electrospray Mass Spectrometric Analysis of Lipid Mediators Derived from Arachidonic Containing Membrane Phospholipids....Pages 497-514
Mass Spectrometry After the Human Genome Project....Pages 515-529
Problems and Prospects in the Characterization of Posttranscriptional Modifications in Large RNAs....Pages 531-551
Back Matter....Pages 553-577