Mass spectrometry and stable isotopes in nutritional and pediatric research

Content: Wiley Series on Mass Spectrometry
 Title Page
 Copyright
 Table of Contents
 List of Contributors
 Introduction
 List of Abbreviations
 Chapter 1: Mass Spectrometry Techniques for In Vivo Stable Isotope Approaches
 1.1 Introduction
 1.2 Nomenclature for Light-Stable Isotope Changes
 1.3 Mass Spectrometry Techniques
 1.4 Choice of Mass Spectrometric Techniques and Applications to Measure Isotopic Enrichments in Metabolic Studies
 1.5 Conclusion and Future Perspectives
 References
 Chapter 2: Stable Isotope Technology
 2.1 History
 2.2 Definition
 2.3 Safety 2.4 Stable Isotopes and Natural Abundances2.5 Stable Isotope Selection
 2.6 Single or Multiple Label Selection
 2.7 Precursor Model
 2.8 Simultaneous Infusion
 2.9 Infusion Techniques
 2.10 Steady State
 2.11 Pool Selection
 2.12 Pool Models
 2.13 Flux: Synthesis and Breakdown
 2.14 Nitrogen Balance
 2.15 Doubly Labeled Water Method
 2.16 Whole-body Protein Synthesis
 2.17 Specific Protein Synthesis
 2.18 Calculations
 2.19 Considerations and Drawbacks of Isotopic Tracers
 2.20 Conclusion
 References
 Chapter 3: Stable Isotopes in Nutritional and Pediatric Research
 3.1 Introduction 3.2 Ethical Aspects3.3 Applications of Stable Isotopes in Nutritional and Pediatric Research
 3.4 Conclusion
 References
 Chapter 4: Early-Life Nutrition and Stable Isotope Techniques
 4.1 Introduction
 4.2 Breast Milk versus Infant Formula
 4.3 Techniques to Monitor Milk Intake
 4.4 Body Composition in Term and Preterm Infants
 4.5 Amino Acid Requirement
 4.6 Clinical Applications
 4.7 Additional Applications
 4.8 Discussion
 4.9 Conclusion
 4.10 Future Perspectives
 References
 Chapter 5: Assessment of Amino Acid Requirement in Children Using Stable Isotopes
 5.1 Introduction 5.2 Nutrient Needs and Definitions5.3 Methods to Determine Requirements
 5.4 Isotopic Tracer Methods
 5.5 Existing Methods to Determine Amino Acid Requirement for Neonates
 5.6 Use of the IAAO Method in the Pediatric Population
 5.7 Necessity for Performing the Study
 5.8 Biochemistry
 5.11 Analysis and Calculations
 5.12 Results
 5.13 Statistical Analysis
 5.14 Discussion
 5.15 Conclusion
 5.16 Future Perspectives
 References
 Chapter 6: Metabolism of Glutamine, Citrulline, and Arginine
 Stable Isotopes Analyzing the Intestinal-Renal Axis
 6.1 Introduction
 6.2 Biochemistry 6.3 Isotopic Model6.4 Study Design
 6.5 Mass Spectrometry Methods
 6.6 Clinical Applications
 6.7 Calculations
 6.8 Discussion and Future Perspectives
 References
 Chapter 7: Applications in Fat Absorption and Metabolism
 7.1 Introduction
 7.2 Biochemistry of Fat Absorption
 7.3 Isotope Model
 7.4 Study Design/Infusion Protocols
 7.5 Analytical Equipment
 7.6 Analytical Conditions
 7.7 Accuracy and Precision
 7.8 Calculations
 7.9 Clinical Applications
 7.10 Future Perspectives
 References
 Chapter 8: Materno-Fetal Lipid Kinetics
 8.1 Introduction