MAP Kinase Signaling Protocols (Methods in Molecular Biology Vol 250)

Front......Page 1
1 The ERK Cascade As a Prototype of MAPK Signaling Pathways......Page 2
2 Determination of ERK Activity......Page 30
3 Detection of ERK1/2 Activities Using Affinity Reagents......Page 50
4 Activation of SAPKs/JNKs and p38s In Vitro......Page 62
5 Investigating the Cellular BMK1/ERK5 Signaling Pathway......Page 90
6 Pull-Down Assays for Guanoside 5'-Triphosphate-Bound Ras-Like Guanosine 5'-Triphosphatases......Page 98
7 Regulation of MAPK Cascades by Protein Tyrosine Phosphatases......Page 104
8 Use of Inhibitors in the Study of MAPK Signaling......Page 114
9 Structure of MAPKs......Page 128
10 Subcellular Localization of MAPKs......Page 146
11 Study of MAPK Signaling Using Knockout Mice......Page 156
12 Computer Simulation of MAPK Signal Transduction......Page 168
13 Signaling by Growth Factor Receptors......Page 178
14 Activation of MAPKs by G Protein-Coupled Receptors......Page 204
15 Identification of MAPK Substrates by Expression Screening With Solid-Phase Phosphorylation......Page 212
16 Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries......Page 238
17 Ras Signaling Pathway for Analysis of Protein–Protein Interactions in Yeast and Mammalian Cells......Page 252
18 Methods in Functional Proteomics......Page 264
19 Practical Methods for Deuterium Exchange/Mass Spectrometry......Page 284
20 Use of Xenopus Oocytes and Early Embryos to Study MAPK Signaling......Page 300
21 MAPK Cascades in the Brain......Page 316