Mammalian Synthetic Promoters

Front Matter ....Pages i-xi
 Initial Considerations Before Designing a Promoter Construct  (David Gould)....Pages 1-7
Front Matter ....Pages 9-9
 Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay  (Nasim Yousaf, David Gould)....Pages 11-21
 Chromatin Immunoprecipitation and Quantitative Real-Time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites Within a Promoter  (Jordan E. Read)....Pages 23-32
 Secreted Reporters for Monitoring Multiple Promoter Function  (Ghazal Lashgari, Rami S. Kantar, Bakhos A. Tannous)....Pages 33-47
 Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo  (Juliette M. K. M. Delhove, Rajvinder Karda, Kate E. Hawkins, Lorna M. FitzPatrick, Simon N. Waddington, Tristan R. McKay)....Pages 49-64
 Monitoring Promoter Activity by Flow Cytometry  (Taher E. I. Taher)....Pages 65-73
Front Matter ....Pages 75-75
 Functional Screening of Core Promoter Activity  (Dan Y. Even, Adi Kedmi, Diana Ideses, Tamar Juven-Gershon)....Pages 77-91
 Bioinformatically Informed Design of Synthetic Mammalian Promoters  (Michael L. Roberts, Polyxeni Katsoupi, Vivian Tseveleki, Era Taoufik)....Pages 93-112
 Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication  (Maria Veronica Lopez, Eduardo G. Cafferata, Diego L. Viale, Osvaldo L. Podhajcer)....Pages 113-130
 Constructing Strong Cell Type-Specific Promoters Through Informed Design  (Adam J. Brown, David C. James)....Pages 131-145
 PCR Assembly of Synthetic Promoters  (Hodan Mohamed, David Gould)....Pages 147-156
Front Matter ....Pages 157-157
 The Tetracycline Responsive System  (Nasim Yousaf, David Gould)....Pages 159-172
 Light-Responsive Promoters  (Maximilian Hörner, Konrad Müller, Wilfried Weber)....Pages 173-186
 A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation  (Ryohei Ogawa, Go Kagiya, Akihiko Watanabe, Akihiro Morii, Zheng-Guo Cui, Takashi Kondo)....Pages 187-203
 Promoter Activation with Electromagnetism  (Abraham O. Rodríguez-De la Fuente, J. Antonio Heredia-Rojas, Juan M. Alcocer-González, Laura E. Rodríguez-Flores, Cristina Rodríguez-Padilla, Reyes S. Taméz-Guerra)....Pages 205-212
 Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment  (Eduardo G. Cafferata, Maria Veronica Lopez, Felipe J. Nuñez, Maria A. R. Maenza, Osvaldo L. Podhajcer)....Pages 213-227
Front Matter ....Pages 229-229
 A Modular Approach to Building Complex Synthetic Circuits  (Yinqing Li, Ron Weiss)....Pages 231-248
 Computational Sequence Design with R2oDNA Designer  (James T. MacDonald, Velia Siciliano)....Pages 249-262
 Design of Synthetic Promoters for Gene Circuits in Mammalian Cells  (Pratik Saxena, Daniel Bojar, Martin Fussenegger)....Pages 263-273
Back Matter ....Pages 275-277