Lipid Metabolism in Signaling Systems

Content: 
Methods in Neurosciences
Page ii

Front Matter
Page iii

Copyright page
Page iv

Contributors to Volume 18
Pages ix-xiii

Preface
Pages xv-xvi
JOHN N. FAIN

Methods in Neurosciences
Page xvii

1 - Phosphatidylinositol Glycan-Anchor-Specific Phospholipase D from Mammalian Brain
Pages 3-13
Marius C. Hoener, Urs Brodbeck

2 - Rat Brain Membrane-Bound Phospholipase D
Pages 14-24
Michal Danin, Vered Chalifa, Heidi Möhn, Uta-Susanne Schmidt, Mordechai Liscovitch

3 - Soluble Phospholipase D
Pages 25-29
M. Motasim Billah, John C. Anthes, Peng Wang

4 - Phosphatidylethanol Formation as Index of Phospholipase D Activity in Rat Brain Cortex Slices
Pages 30-37
Silvia Llahi, John N. Fain

5 - Agonist Stimulation of Phosphoinositide Breakdown in Brain Membranes
Pages 38-48
Enrique Claro, Fernando Picatoste, John N. Fain

6 - Regulation of Phosphoinositide Metabolism in Membranes Prepared from Human Brain Cortex
Pages 49-57
Michael A. Wallace

7 - Purification of Phospholipase C-β3 from Rat Brain
Pages 58-63
Deok-Young Jhon, Dongeun Park, Sue Goo Rhee

8 - Purification of Phospholipase C-β4 and Phospholipase C-δ2 from Bovine Retinas
Pages 64-71
Chang-Won Lee, Kweon-Haeng Lee, Sue Goo Rhee

9 - Purification of αq/11 from Brain
Pages 72-82
Alan V. Smrcka, Paul C. Sternweis

10 - Purification of Phosphatidylinositol Synthase from Brain
Pages 85-92
Abdallah Ghalayini, Joseph Eichberg

11 - Phosphatidylinositol 4-Kinase from Bovine Brain
Pages 93-99
Akio Yamakawa, Tadaomi Takenawa

12 - Phosphatidylinositol 3-Kinase
Pages 100-112
Stephen P. Soltoff, David R. Kaplan, Lewis C. Cantley

13 - Identification of Phosphatidylinositol Trisphosphate in Rat Brain
Pages 113-124
Ranganathan Parthasarathy, Lathakumari Parthasarathy, Robert E. Vadnal

14 - Preparation of Protein Kinase C Isozymes and Substrates from Rat Brain
Pages 127-137
Freesia L. Huang, Kuo-Ping Huang, Fwu-Shan Sheu, Ken-ichi Osada

15 - Protein Kinase C, a Zinc Metalloprotein: Quantitation of Zinc by Atomic Absorption Spectrometry
Pages 138-153
Andrew F.G. Quest, Elaine S.G. Bardes, John Bloomenthal, Roy A. Borchardt, Robert M. Bell

16 - Expression of Protein Kinase C Isozymes in Insect Cells and Isolation of Recombinant Proteins
Pages 154-173
Silvia Stabel, Marek Liyanage, David Frith

17 - Immunochemical Localization of Protein Kinase C and Phosphoinositide-Specific Phospholipase C
Pages 174-189
Susan Jake, Karen Leach

18 - Quantitative Analysis of Molecular Species of Diacylglycerol in Biological Samples
Pages 190-198
Chunghee Lee, Amiya K. Hajra

19 - Measurement of Inositol Trisphosphate by Gas Chromatography/Mass Spectrometry: Femtomole Sensitivity Provided by Negative-Ion Chemical Ionization Mass Spectrometry in Submilligram Quantities of Tissue
Pages 201-212
Leona J. Rubin, Fong-Fu Hsu, William R. Sherman

20 - Characterization of Inositol Phosphates by High-Performance Liquid Chromatography
Pages 213-223
Nobuyuki Sasakawa, Toshio Nakaki, Ryuichi Kato

21 - Measurement of Inositol 1,4,5-Trisphosphate, Inositol 1,3,4,5-Tetrakisphosphate, and Phosphatidylinositol 4,5-Bisphosphate in Brain
Pages 224-244
R.A. John Challiss, Stefan R. Nahorski

22 - Molecular Analysis of Inositol 1,4,5-Trisphosphate Receptors
Pages 247-265
Gregory A. Mignery, Thomas C. Südhof

23 - Inositol 1,4,5-Trisphosphate Receptor Down-Regulation
Pages 266-279
Richard J.H. Wojcikiewicz, Stefan R. Nahorski

24 - High-Affinity Inositol 1,3,4,5-Tetrakisphosphate Receptor from Cerebellum
Pages 280-297
Georg Reiser

25 - Inositol 1,4,5-Trisphosphate-Binding Proteins in Rat Brain Cytosol
Pages 298-311
Masato Hirata, Takashi Kanematsu

26 - Inositol 1,4,5-Trisphosphate Phosphatase and Kinase from Brain
Pages 312-319
Christophe Erneux, Kazunaga Takazawa, Benoît Verjans

27 - Synthesis of 32P-Labeled Phosphoinositides and Inositol Phosphates: Characterization and Purification of Inositol 1,3,4,5-Tetrakisphosphate-3-phosphatase from Brain
Pages 320-336
Ariane Höer, Eckard Oberdisse

28 - Species Differences in the Response of Second Messenger Inositol 1,4,5-Trisphosphate to Lithium
Pages 337-352
Lowell E. Hokin, John F. Dixon

Index
Pages 353-357