Lectins and Glycobiology

Front Matter....Pages I-XX
Front Matter....Pages 1-1
Lectins: Insights into the State of Knowledge by Literature Searches....Pages 3-5
Front Matter....Pages 7-7
Synthetic Ligands for Lectins....Pages 9-22
Sugar-Polyacrylamide Conjugates as Probes for Cell Lectins....Pages 23-28
Front Matter....Pages 29-29
Isolation of Plant Lectins....Pages 31-46
A General Procedure for the Purification of Fimbrial Lectins from Escherichia Coli ....Pages 47-54
Isolation, Detection, and Ligand Binding Specificity of a Lectin Uniquely Found in Rat Kupffer Cells....Pages 55-64
Galaptin: Isolation, Electrophoretic Analysis, Peptide Preparation for Sequencing, and Carbohydrate-Binding Site Specificity....Pages 65-80
Determination of Carbohydrate Specificity in Solid-Phase Assays....Pages 81-85
Analysis of Isoelectric Variants of Carbohydrate Binding Protein 35....Pages 86-97
Chemical Modification of Lectins by Group-Specific Reagents....Pages 98-101
Front Matter....Pages 103-103
Study of Oligosaccharide-Lectin Interaction by Various Nuclear Magnetic Resonance (NMR) Techniques and Computational Methods....Pages 105-116
Lectin-Glycoconjugate Cross-Linking Interactions....Pages 117-128
Lectins as Analytical Probes to Define the Physical Characteristics of Binding Events....Pages 129-137
Front Matter....Pages 139-139
Glycoprotein-Lectin-Immunosorbent Assay (GLIA)....Pages 141-149
Use of Lectins in Quantification and Characterization of Soluble Glycoproteins....Pages 151-157
Lectins as Indicators of Disease-Associated Glycoforms....Pages 158-174
Viral Lectins for the Detection of 9-O-Acetylated Sialic Acid on Glycoproteins and Glycolipids....Pages 175-181
Affinoblotting Detection of the Glycoprotein Ligands of the Endogenous Cerebellar Soluble Lectin CSL and of Concanavalin A....Pages 182-187
Front Matter....Pages 189-189
An Enzyme Immunoassay (EIA) for Human Endogenous β -Galactoside-Binding Lectin....Pages 191-195
Analysis of Lectin Expression by Immunoblotting....Pages 196-201
Front Matter....Pages 189-189
Application of Immuno- and Affinocytochemistry to the Detection of an Endogenous β -Galactoside-Specific 14 kDa Lectin and Its Ligands....Pages 202-208
Front Matter....Pages 209-209
Tissue Lectins in Histopathology — Markers in Search of Their Physiological Ligands....Pages 211-214
Lectin Cytochemistry Using Colloidal Gold Methodology....Pages 215-233
Neoglycoprotein-Gold Complexes as a Tool in the Study of Carbohydrate-Specific Binding Proteins....Pages 234-240
Lectins and Neoglycoproteins — Attractive Molecules to Study Glycoconjugates and Accessible Sugar-Binding Sites of Lower Vertebrates Histochemically....Pages 241-250
In Situ Microdensitometry of Neoglycoprotein Staining: Detection of Tissue-Site Specific Patterns of Endogenous Lectin Expression....Pages 251-259
Lectin and Neoglycoprotein Binding to Cells....Pages 260-271
Use of Lectins for the Phenotypic Characterization of Cultured Cell Monolayers....Pages 272-277
The Use of Lectins in the Purification of Cellular Subpopulations....Pages 278-286
Lectin-Binding Properties of Extracellular Matrix in the Chick Oral Membrane....Pages 287-294
Biodistribution of Radioactive Lectins....Pages 295-303
Lectin Interactions with Intestinal Epithelial Cells and Their Potential for Drug Delivery....Pages 304-318
Neoglycoprotein-Liposome Conjugates for Studies of Membrane Lectins....Pages 319-326
Front Matter....Pages 327-327
Effects of Lectins on Adenylylation and Phosphorylation of Plasma Membrane Proteins....Pages 329-355
Lectin-Induced Alterations in the Level of Phospholipids, Inositol Phosphates, and Phosphoproteins....Pages 356-361
Measurement of Intracellular Calcium Levels by Flow Cytometry Following Treatment of Murine Macrophage/Monocytes with Mistletoe Lectin....Pages 362-364
Measurement of Lectin-Induced Superoxide Release from Human Neutrophils....Pages 365-368
Isolation of Lymphocytes and Accessory Cells for Assays of Mitogenicity....Pages 369-375
Determination of Lectin-Mediated Augmentation of Natural Killer Cell-Mediated Cytotoxicity....Pages 376-379
Effects of Pseudomonas aeruginosa PA-I and PA-II Lectins on Tumoral Cells....Pages 380-395
Front Matter....Pages 327-327
Determination of Lectin-Dependent Alterations of Cellular Parameters by Immunophenotyping During Adjuvant Lectin Application....Pages 396-401
Lectin-Dependent Alteration in Availability of Nutrients in Serum and Erythrocytes....Pages 402-404
Front Matter....Pages 405-405
Bacterial Adhesion to Immobilized Carbohydrates....Pages 407-410
Quantitative Micro-Adhesion Assay on Polystyrene Matrices....Pages 411-417
Lectin-Mediated Binding Activities of Bradyrhizobium japonicum ....Pages 418-424
Identifying a Role for Carbohydrate Recognition in Regulating Neuronal Architecture....Pages 425-432
Identifying Cell-Cell Adhesion-Inhibitory Antibodies to Cell Surface Proteins Using Divalent Primary and Monovalent Secondary Antibodies — a Method Developed Using Cellular Slime Molds....Pages 433-440
A Modified Capillary Method for Measuring Bacterial Chemotaxis....Pages 441-444
Front Matter....Pages 445-445
Cloning Lectin Genes....Pages 447-457
Cell-Free Synthesis of Lectins....Pages 458-468
cDNA Cloning and Expression of Plant Lectins from the Legume Family....Pages 469-473
Production of Intact Recombinant Lectins in Escherichia coli ....Pages 474-481
Construction of Mutant Lectin Genes by Oligonucleotide-Directed Mutagenesis and Their Expression in Escherichia coli ....Pages 482-491
Mammalian Lectin as Transforming Growth Factor....Pages 492-499
Identification and Expression of a Protozoan Invasion Factor: Penetrin, the Heparin-Binding Protein from Trypanosoma cruzi ....Pages 500-512
Back Matter....Pages 513-521