Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate

Content: 
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xv-xvii

Miscellaneous
Page xix

Preface
Page xxi
Jon Lorsch

Methods in Enzymology
Pages xxiii-lix

Chapter One - Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies
Pages 3-14
Elizabeth Eyler

Chapter Two - Storage of Bacteria and Yeast
Pages 15-21
Cheryl M. Koh

Chapter Three - Cellular Fractionation – Mammalian Cells
Pages 25-30
Joerg von Hagen, Uwe Michelsen

Chapter Four - Cellular Fractionation – Yeast Cells
Pages 31-39
Joerg von Hagen, Uwe Michelsen

Chapter Five - Yeast-Gene Replacement Using PCR Products
Pages 43-55
Megan Bergkessel, Christine Guthrie, John Abelson

Chapter Six - Measuring RNAi Knockdown using qPCR
Pages 57-77
Stuart Milstein, Michael Nguyen, Rachel Meyers, Antonin de Fougerolles

Chapter Seven - Recombineering: Using Drug Cassettes to Knock out Genes in vivo
Pages 79-102
James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, Donald L. Court

Chapter Eight - Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae
Pages 103-131
Samantha Stuckey, Francesca Storici

Chapter Nine - Gene Targeting and Site-Specific Recombination in Mouse ES Cells
Pages 133-155
Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, A. Francis Stewart

Chapter Ten - Recombineering: Highly Efficient in vivo Genetic Engineering using Single-strand Oligos
Pages 157-177
James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, Donald L. Court

Chapter Eleven - Growth Media for E. coli
Pages 181-189
Juliane C. Lessard

Chapter Twelve - Saccharomyces Cerevisiae Growth Media
Pages 191-204
Jessica S. Dymond

Chapter Thirteen - Immunohistochemistry on Freely Floating Fixed Tissue Sections
Pages 207-215
Julia Bachman

Chapter Fourteen - Preparation of Slides for Microscopy from Frozen Tissue Sections
Pages 217-224
Julia Bachman

Chapter Fifteen - Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry
Pages 225-233
Kirstie Canene-Adams

Chapter Sixteen - Preparation of Cells for Microscopy using Cytospin
Pages 235-240
Cheryl M. Koh

Chapter Seventeen - Preparation of Cells for Microscopy using Chamber Slides and Coverslips
Pages 241-247
Cheryl M. Koh

Chapter Eighteen - Preparation of Cells for Microscopy using ‘Cell Blocks’
Pages 249-255
Cheryl M. Koh

Chapter Nineteen - Enzymatic Deglycosylation of Glycoproteins
Pages 259-263
Min-Sung Kim, Dan Leahy

Chapter Twenty - Preparation of Fatty Acid or Phospholipid Vesicles by Thin-film Rehydration
Pages 267-274
Ting F. Zhu, Itay Budin, Jack W. Szostak

Chapter Twenty-one - Vesicle Extrusion Through Polycarbonate Track-etched Membranes using a Hand-held Mini-extruder
Pages 275-282
Ting F. Zhu, Itay Budin, Jack W. Szostak

Chapter Twenty-two - Preparation of Fatty Acid Micelles
Pages 283-288
Ting F. Zhu, Itay Budin, Jack W. Szostak

Chapter Twenty-three - Reverse-phase HPLC Analysis and Purification of Small Molecules
Pages 291-301
Kirstie Canene-Adams

Chapter Twenty-Four - Thin Layer Chromatography
Pages 303-324
Marina Santiago, Scott Strobel

Chapter Twenty-five - ATP and GTP Hydrolysis Assays (TLC)
Pages 325-334
Vaishnavi Rajagopal, Jon R. Lorsch

Author Index
Pages 335-337

Subject Index
Pages 339-347