Laboratory Manual for Microbiology Fundamentals: A Clinical Approach

Cover
Title
Copyright
About the Authors
Contents
Laboratory Overview: Introduction
Laboratory Safety, Equipment, and Materials
Tools of the Laboratory: Basic Techniques
	1 Use of the Microscope
	2 Aseptic Techniques
	3 Isolation Streak Plate
Eukaryotic Cells and Microorganisms
	4 Microbial Phototrophs: Algae and Cyanobacteria
	5 Protozoa
	6 Fungi
	7 Bacterial Motility: Hanging Drop Method
Methods for Microscopic Analysis: Staining
	8 Simple Staining and Smear Preparation
	9 Negative Stain
	10 Capsule Stain
	11 Gram Stain
	12 Acid-Fast Stain (Ziehl-Neelsen and Kinyoun Methods)
	13 Endospore Stain (Schaeffer-Fulton Method)
Methods for Culturing and Diagnosing Microbes: Media
	14 Blood Agar
	15 Mannitol Salt Agar
	16 Eosin Methylene Blue Agar
	17 MacConkey Agar
Microbial Nutrition, Ecology, and Growth
	18 Nutrients and Growth: Chemical Content of Media
	19 Osmotic Pressure and Growth
	20 Oxygen and Growth: Aerotolerance
	21 Oxygen and Growth: Catalase
	22 Oxygen and Growth: Oxidase
Food and Water Safety
	23 Plate Count
Treatment and Control of Microbial Growth
	24 Killing by Ultraviolet Light
	25 Antimicrobial Drug Susceptibility Testing (Kirby-Bauer Method)
Microbial Genetics and Genetic Engineering
	26 ELISA and Using Nucleotide BLAST (Dry Lab)
	27 Analysis of DNA: Electrophoresis
	28 Gene Transfer: Transformation
Infectious Diseases
	29 Parasitic Protozoa
	30 Parasitic Worms
	31 Transmission: Vectors of Disease
	32 Transmission: Epidemiology Activity
Diagnosing Infections
	33 Gel Immunoprecipitation (Immunodiffusion)
	34 Passive (Indirect) Agglutination
	35 Rapid Immunoassays
	36 Hemagglutinin Inhibition Assay
	37 EnteroPluri-Test
Environmental Microbiology
	38 Soil Microbiology
	39 Nitrate Reduction
Hydrolytic Enzymes and Disease Mechanisms
	40 DNase Test
	41 Gelatin Hydrolysis
	42 Starch Hydrolysis
	43 Casein Hydrolysis
Biochemical Testing and Diagnosis of Disease
	44 Phenol Red Sugar Broth
	45 Triple-Sugar Iron Agar
	46 Litmus Milk
	47 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) Reactions
	48 Urease Test
Putting It All Together
	49 "Known" Bacteria for Biochemical Testing
	50 Identification of Unknown Bacteria
Index
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	P
	Q
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	X
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