Immunoproteomics: Methods and Protocols

Front Matter ....Pages i-xi
 Introduction to the Immune System  (Scott McComb, Aude Thiriot, Bassel Akache, Lakshmi Krishnan, Felicity Stark)....Pages 1-24
 Immunoproteomics Methods and Techniques  (Kelly M. Fulton, Isabel Baltat, Susan M. Twine)....Pages 25-58
 Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification  (Kelly M. Fulton, Anna Ananchenko, Lawrence Wolfraim, Shannon Martin, Susan M. Twine)....Pages 59-78
 Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology  (Greg Harris, Wangxue Chen)....Pages 79-94
 Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis  (Timothy D. Veenstra)....Pages 95-102
 Enriching for Low-Abundance Serum Proteins Using ProteoMiner™ and Protein-Level HPLC  (Sophie Moggridge, Kelly M. Fulton, Susan M. Twine)....Pages 103-117
 Identification of the Antigen Content of Electroimmunoprecipitates  (N. Helena Beyer, Niels H. H. Heegaard)....Pages 119-135
 Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay  (Christine M. Tin, Stanislav V. Sosnovtsev)....Pages 137-152
 Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum  (Christie E. Delaney, John F. Kelly, Wen Ding, Arsalan S. Haqqani)....Pages 153-166
 Antigen Identification for Cell-Binding Antibodies Using Ligand-Directed Crosslinking and Biotin Transfer  (Tammy-Lynn Tremblay, Jennifer J. Hill)....Pages 167-180
 Whole-Genome Phage Display Libraries: A Powerful Tool for Antigen Discovery  (Elisa Beghetto, Nicola Gargano)....Pages 181-198
 Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray  (Johannes W. Pedersen, Alexander Nøstdal, Hans H. Wandall)....Pages 199-211
 Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification  (Fei Wen, Mason R. Smith, Huimin Zhao)....Pages 213-234
 Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification  (Sarah A. Penny, Stacy A. Malaker)....Pages 235-243
 Enhanced Mass Spectrometry Detection of MHC Peptides  (Rui Chen, Jianjun Li)....Pages 245-257
 Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide Particles  (Rui Chen, Jianjun Li)....Pages 259-268
 Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways  (Stacy A. Malaker, Michael J. Ferracane)....Pages 269-285
 Genome-Based Bioinformatic Prediction of Major Histocompatibility (MHC)  (Simon J. Foote)....Pages 287-300
 Enhancing Mass Spectrometry-Based MHC-I Peptide Identification Through a Targeted Database Search Approach  (Prathyusha Konda, J. Patrick Murphy, Morten Nielsen, Shashi Gujar)....Pages 301-307
 Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics  (Arsalan S. Haqqani, Danica B. Stanimirovic)....Pages 309-325
 Genomics-Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases  (Raghavendra Giri, Veneta Qendro, Pooja Rani, Carren Jepchumba, Grace Bugos, Volker Stadler et al.)....Pages 327-332
 Identification of Antigens in Immune Complexes  (Nozomi Aibara, Kaname Ohyama)....Pages 333-338
 Analysis and Characterization of Immune Cells and Their Activation Status by Whole-Cell MALDI-TOF Mass Spectrometry  (Richard Ouedraogo, Julien Textoris, Laurent Gorvel, Aurélie Daumas, Christian Capo, Jean-Louis Mege)....Pages 339-351
 A Molecular Immunoproteomics Approach to Assess the Viral Antigenicity of Influenza  (Kevin M. Downard)....Pages 353-369
 Immunoproteomic Biomarkers: From Publication to Personalized Medicine  (Devanand M. Pinto)....Pages 371-377
Back Matter ....Pages 379-380