Immunochemical Techniques Part I: Hybridoma Technology and Monoclonal Antibodies

Content: 
Contributors to volume 121
Pages xiii-xix

Preface
Page xxi
John J. Langone, Helen Van Vunakis

Volumes in series
Pages xxiii,xxv-xxxiv

[1] Factors affecting production of monoclonal antibodies Original Research Article
Pages 3-18
Regine J. Westerwoudt

[2] In vitro immunization for the production of antigen-specific lymphocyte hybridomas Original Research Article
Pages 18-27
Christopher L. Reading

[3] An improved in vitro immunization procedure for the production of monoclonal antibodies Original Research Article
Pages 27-33
Barbara D. Boss

[4] “Single-shot” intrasplenic immunization for the production of monoclonal antibodies Original Research Article
Pages 33-41
M. Spitz

[5] Method for generating a high frequency of hybridomas producing monoclonal IgA antibodies Original Research Article
Pages 42-51
Dawn E. Colwell, Suzanne M. Michalek, Jerry R. McGhee

[6] Production of IgA-secreting rat Г— rat hybridomas Original Research Article
Pages 52-59
C.J. Dean, L.A. Gyure, J.G. Hall, J.M. Styles

[7] The use of conventional antisera in the production of specific monoclonal antibodies Original Research Article
Pages 59-69
Kendra B. Eager, Roger H. Kennett

[8] Preparation of monoclonal antibodies to preselected protein regions Original Research Article
Pages 69-95
M. Zouhair Atassi

[9] The auto-anti-idiotypic strategy for preparing monoclonal antibodies to receptor combining sites Original Research Article
Pages 95-107
W. Louis Cleveland, Bernard F. Erlanger

[10] Optimal strategies for developing human—human monoclonal antibodies Original Research Article
Pages 107-119
Paul G. Abrams, Jeffrey L. Rossio, H.C. Stevenson, K.A. Foon

[11] Comparative phenotypic analysis of available human hybridoma fusion partners Original Research Article
Pages 120-140
Danuta Kozbor, John C. Roder, Michael E. Sierzega, Susan P.C. Cole, Carlo M. Croce

[12] The EBV-hybridoma technique Original Research Article
Pages 140-167
J.C. Roder, S.P.C. Cole, D. Kozbor

[13] Generation of human monoclonal antibodies by fusion of EBV-activated B cells to a human-mouse hybridoma Original Research Article
Pages 168-174
Steven K.H. Foung, Edgar G. Engleman, F. Carl Grumet

[14] Improved hybridoma technology: Spleen cell separation and soluble growth factors Original Research Article
Pages 174-182
P. Van Mourik, W.P. Zeijlemaker

[15] High efficiency fusion procedure for producing monoclonal antibodies against weak immunogens Original Research Article
Pages 183-192
Richard D. Lane, Robert S. Crissman, Stephen Ginn

[16] Methods for high frequency production of soluble antigen-specific hybridomas; specificities and affinities of the monoclonal antibodies obtained Original Research Article
Pages 193-210
Maurizio Cianfriglia, Massimo Mariani, Doretta Armellini, Annalisa Massone, Margherita Lafata, Rivo Presentini, Guido Antoni

[17] Bispecific monoclonal antibodies from hybrid hybridomas Original Research Article
Pages 210-228
M.R. Suresh, A.C. Cuello, C. Milstein

[18] Human Г— (mouse Г— human) hybridomas Original Research Article
Pages 228-234
Lars Г–stberg

[19] Generation of rat-rat hybridomas with the use of the LOU IR983F nonsecreting fusion cell line Original Research Article
Pages 234-238
Lieve De Clercq, Françoise Cormont, Hervé Bazin

[20] Production of Syrian and Armenian hamster monoclonal antibodies of defined specificity Original Research Article
Pages 239-244
Francisco Sanchez-Madrid, Timothy A. Springer

[21] Production and characterization of bovine immunoglobulins from bovine Г— murine hybridomas Original Research Article
Pages 244-265
Albert J. Guidry, S. Srikumaran, Richard A. Goldsby

[22] Serum-free medium for the growth of NS-1 mouse myeloma cells and the isolation of NS-1 hybridomas Original Research Article
Pages 266-277
Tomoyuki Kawamoto, J. Denry Sato, Don B. McClure, Gordon H. Sato

[23] Serum-free medium for hybridoma and parental myeloma cell cultivation Original Research Article
Pages 277-292
Jan KovГЎЕ™, FrantiЕЎek FranД›k

[24] Endothelial cell growth supplements for promoting the growth of monoclonal antibody-producing hybridoma cells Original Research Article
Pages 293-295
Janet H. Ransom

[25] Feeder effect of enriched human monocytes on human hybridoma, myeloma, and lymphoma cell lines Original Research Article
Pages 295-300
Thomas Brodin, Lennart Olsson, Hans Olov Sjögren

[26] Removal of bovine immunoglobulin from serum in hybridoma culture media using protein A Original Research Article
Pages 301-306
P.A. Underwood

[27] A single-step technique for selecting and cloning hybridomas for monoclonal antibody production Original Research Article
Pages 307-322
John M. Davis

[28] Cloning of murine hybridoma cells in ultra-low gelation temperature agarose Original Research Article
Pages 322-327
Curt I. Civin, M.L. Banquerigo

[29] A simple method for cloning hybridomas in 20-Ојl hanging drops Original Research Article
Pages 327-331
Marvin B. Rittenberg, Abigail Buenafe, McKay Brown

[30] Clonal isolation of hybridomas by manual single-cell isolation Original Research Article
Pages 332-340
Albert BoeyГ©

[31] Semiautomated plaque method for the detection of antibody-forming cell clones Original Research Article
Pages 341-352
Beverley L. Pike

[32] Production of monoclonal antibodies by agarose-entrapped hybridoma cells Original Research Article
Pages 352-360
K. Nilsson, W. Scheirer, H.W.D. Katinger, K. Mosbach

[33] Automated production of monoclonal antibodies in a cytostat Original Research Article
Pages 360-375
S. Fazekas De St. Groth

[34] The effect of pristane on ascites tumor formation and monoclonal antibody production Original Research Article
Pages 375-381
Nicholas J. Hoogenraad, Christopher J. Wraight

[35] Production of ascites and rescue of hybridomas by intrasplenic inoculation Original Research Article
Pages 381-385
Pamela L. Witte, Rosalie Ber

[36] Growth of rat-mouse hybridomas in nude mice and nude rats Original Research Article
Pages 386-411
Dhirendra N. Misra, Saad A. Noeman, Heinz W. Kunz, Thomas J. Gill III

[37] Poisson statistical analysis of repetitive subcloning by the limiting dilution technique as a way of assessing hybridoma monoclonality Original Research Article
Pages 412-417
Hilary A. Coller, Barry S. Coller

[38] A method for freezing hybridoma clones in 96-well microculture plates Original Research Article
Pages 417-422
David E. Wells, William F. Bibb

[39] Multivalent antibody radioimmunoassay (MARIA) for screening specific antibody secretion by lymphocyte hybridoma cultures Original Research Article
Pages 425-433
J. Gheuens, D.E. McFarlin

[40] A rapid technique using radiolabeled soluble antigen to screen hybridoma culture supernatants Original Research Article
Pages 433-437
K.F. Miller, D.J. Bolt, R.A. Goldsby

[41] Culture-well solid-phase 3H-RIA for the isotype-specific measurement of antibody from mouse and human antibody-secreting cells Original Research Article
Pages 438-453
Patricia K.A. Mongini, Clare Blessinger

[42] The spaghetti overlay procedure Original Research Article
Pages 453-459
W. Steven Adair

[43] Solution-phase RIA and solid-phase EIA using avidin-biotin systems for analysis of monoclonal antibody epitopes and affinity constants Original Research Article
Pages 459-472
John E. Shively, Christoph Wagener, Brian R. Clark

[44] A simple method for ranking the affinities of monoclonal antibodies Original Research Article
Pages 472-481
Veronica Van Heyningen

[45] The hazards of mycoplasma contamination in the screening of hybridoma supernatants for effects on [3H]thymidine incorporation Original Research Article
Pages 481-484
James P. Jakway

[46] The dot immunobinding assay Original Research Article
Pages 484-491
Richard Hawkes

[47] Detection of antibody-secreting hybridomas with diazobenzyloxymethyl paper Original Research Article
Pages 491-497
GeneviГЁve Motta, Daniel Locker

[48] The use of alkaline phosphatase-conjugated anti-immunoglobulin with immunoblots for determining the specificity of monoclonal antibodies to protein mixtures Original Research Article
Pages 497-509
Peter L. Ey, Leonie K. Ashman

[49] Slide immunoenzymatic assay (SIA) in hybridoma technology Original Research Article
Pages 509-525
Everly Conway De Macario, Alberto J.L. Macario, Robert J. Jovell

[50] Use of mouse and human monoclonal antibodies in enzyme immunofiltration Original Research Article
Pages 525-532,IN1,533-541
Mark C Glassy, Patrick H. Cleveland

[51] Measurement of monoclonal immunoglobulin concentrations in hybridoma cultures by competitive inhibition enzyme immunoassay Original Research Article
Pages 541-547
Kenneth W. Hunter Jr., John M. Bosworth Jr.

[52] The detection and characterization of antigen-specific monoclonal antibodies using anti-immunoglobulin isotype antibodies coupled to red blood cells Original Research Article
Pages 548-556
M.R. Clark

[53] Agglutination assay using protein A-sensitized erythrocytes for detection of immunoglobulin in tissue culture supernatants Original Research Article
Pages 556-561
David Male, Gareth Pryce

[54] Immunohistochemical techniques using monoclonal antibodies Original Research Article
Pages 562-579
Daniel R. Ciocca, Robert J. Bjercke

[55] Method for rapid detection of membrane antigens by immunofluorescence and its application to screening monoclonal antibodies Original Research Article
Pages 580-587
Claude Boucheix, Patricia Krief, Jean-Yves Perrot, Massoud Mirshahi, Claude Rosenfeld

[56] Purification of mouse monoclonal antibodies from ascitic fluid by DEAE affi-gel blue chromatography Original Research Article
Pages 587-596
Claudine Bruck, Jeffrey A. Drebin, Corinne Glineur, Daniel Portetelle

[57] Purification and analysis of monoclonal antibodies by high-performance liquid chromatography Original Research Article
Pages 596-615
Scott W. Burchiel

[58] Separation of IgG idiotypes by high-performance hydroxylapatite chromatography Original Research Article
Pages 615-622
Hector Juarez-Salinas, Gary S. Ott, Jeng-Chyh Chen, Timothy L. Brooks, Larry H. Stanker

[59] The use of rat monoclonal antibodies to characterize, quantify, and purify polyclonal or monoclonal mouse IgM Original Research Article
Pages 622-631
Françoise Cormont, Patrick Manouvriez, Lieve De Clercq, Hervé Bazin

[60] Purification of IgG monoclonal antibodies from ascitic fluid based on rivanol precipitation Original Research Article
Pages 631-638
FrantiЕЎek FranД›k

[61] Purification of rat monoclonal antibodies Original Research Article
Pages 638-652
Hervé Bazin, Françoise Cormont, Lieve De Clercq

[62] Preparation of F(ab′)2 fragments from mouse IgG of various subclasses Original Research Article
Pages 652-663
Edmundo Lamoyi

[63] Optimal conditions for the preparation of proteolytic fragments from monoclonal IgG of different rat IgG subclasses Original Research Article
Pages 663-669
J. Rousseaux, R. Rousseaux-Prevost, H. Bazin

[64] Tritium radiolabeling of antibodies to high specific activity with N-succinimidyl [2,3-3H]propionate: Use in detecting and analyzing monoclonal antibodies Original Research Article
Pages 670-678
Udo Kummer

[65] Determination of the true immunoreactive fraction of monoclonal antibodies after radiolabeling Original Research Article
Pages 678-691
Tore Lindmo, Paul A. Bunn Jr.

[66] Ultrasensitive cooperative immunoassays with mixed monoclonal antibodies Original Research Article
Pages 695-702
Paul H. Ehrlich, William R. Moyle

[67] Use of monoclonal antibody to increase the sensitivity and specificity of precipitating immunoassays and cell surface binding immunoassays Original Research Article
Pages 702-717
Robert R. Montgomery, Thomas J. Kunicki, L. Michael Glode

[68] Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and streptavidin-agarose Original Research Article
Pages 717-725
Timothy V. Updyke, Garth L. Nicolson

[69] An indirect rosette technique for the identification and separation of lymphocyte populations by monoclonal antibodies Original Research Article
Pages 726-737
Kingston Mills

[70] Separation of T cell subpopulations by monoclonal antibodies and affinity chromatography Original Research Article
Pages 737-748
RГјdiger W. Braun, Gunther KГјmel

[71] A rapid micromethod for the enumeration of T cell subsets in blood by epifluorescence microscopy Original Research Article
Pages 748-758
Ian H. Frazer, Ian R. Mackay, Frank Battye

[72] Cytotoxicity assays based on the use of antibody secretion as a measure of hybridoma viability Original Research Article
Pages 759-771
J. Marbrook

[73] Use of iron- or selenium-coupled monoclonal antibodies to cell surface antigens as a positive selection system for cells Original Research Article
Pages 771-783
Mark Bothwell, Timothy Block

[74] Screening for monoclonal antibodies to human cellular and soluble antigens Original Research Article
Pages 783-796
Daniel P. Eskinazi, William C. Eby, Giuseppe A. Molinaro

[75] Epitope ratio analysis for the simultaneous study of several cell surface antigens Original Research Article
Pages 797-801
Bennett Kaufman, Richard Goldsby

[76] Use of monoclonal antibodies as radiopharmaceuticals for the localization of human carcinoma xenografts in athymic mice Original Research Article
Pages 802-816
David Colcher, Jose Esteban, Françoise Mornex

[77] Coating of liposomes with subunits of monoclonal IgM antibody and targeting of the liposomes Original Research Article
Pages 817-828
Yoshiyuki Hashimoto, Minoru Sugawara, Toshio Kamiya, Shinya Suzuki

[78] Immunohistological studies with monoclonal antibodies Original Research Article
Pages 828-848
Wayne W. Hancock, Robert C. Atkins

[79] Use of alkaline phosphatase-conjugated antibodies for detection of protein antigens on nitrocellulose filters Original Research Article
Pages 848-855
Bryan M. Turner

[80] Stepwise amplified immunoenzyme staining techniques for the detection of monoclonal antibodies and antigens Original Research Article
Pages 855-867
P.M. Lansdorp, A.W. Wognum, W.P. Zeijlemaker

[81] Other articles from methods in enzymology related to immunochemical techniques
Pages 871-876
Helen Van Vunakis, John J. Langone

Author index
Pages 877-909

Subject index
Pages 911-947