Immunobiology of HLA: Volume I Histocompatibility Testing 1987

Front Matter....Pages i-liv
Front Matter....Pages 1-1
Overview of Experimental Design for the Tenth International Histocompatibility Workshop....Pages 3-8
Front Matter....Pages 9-9
Description of the Reference Panel of B-Lymphoblastoid Cell Lines for Factors of the HLA System: The B-Cell Line Panel Designed for the Tenth International Histocompatibility Workshop....Pages 11-19
Serologic Characterization of the Reference Panel of B-Lymphoblastoid Cell Lines for Factors of the HLA System....Pages 19-38
HLA-Dw and HLA-DP Typing of the Reference Panel of B-Lymphoblastoid Cell Lines....Pages 38-42
Assignment of HLA-A and HLA-B Antigens for the Reference Panel of B-Lymphoblastoid Cell Lines Determined by One-Dimensional Isoelectric Focusing (ID-IEF) Gel Electrophoresis....Pages 43-44
Assignment of HLA-Class II α and β Chain 2-D Gel Patterns for the Workshop Reference Panel of B-Lymphoblastoid Cell Lines....Pages 44-46
Assignment of T-Cell-Defined (TCD) HLA Class II Specificities for the Reference Panel of B-Lymphoblastoid Cell Lines....Pages 46-48
Front Matter....Pages 49-49
New HLA Specificities and Provisional HLA Determinants Defined by the Tenth International Histocompatibility Workshop....Pages 51-51
New HLA Antigens Identified in the Serologic Studies of the Tenth International Histocompatibility Workshop, 1987....Pages 52-53
Nomenclature for HLA-A and HLA-B Alleles Detected by One-Dimensional Isoelectric Focusing (1D-IEF) Gel Electrophoresis....Pages 54-57
Nomenclature for Two-Dimensional (2-D) Gel Patterns of the HLA Class II α and β Chains Defined in the Tenth Workshop....Pages 58-61
Nomenclature for T-Cell-Defined HLA Class II Determinants....Pages 62-67
Nomenclature for HLA-RFLP....Pages 67-71
Nomenclature for Factors of the HLA System, 1987....Pages 72-79
Front Matter....Pages 81-81
Tenth International Histocompatibility Workshop: Overview of Data Processing and Analysis....Pages 83-85
Display of Correlation Structures....Pages 85-90
Front Matter....Pages 91-91
Serologic Exercises of the Tenth Workshop....Pages 93-106
Data Processing and Serum Analysis....Pages 106-110
Pattern Analysis....Pages 110-114
Cell Typing....Pages 114-116
Front Matter....Pages 91-91
Antigen Society #1 Report (A1, Aw36, A3, A11, A9, A23, A24)....Pages 116-119
Antigen Society #2 Report (A25, A26, AW34, AW43 and AW66)....Pages 119-123
Antigen Society #3 Report (AW19 and A32/Aw74)....Pages 124-126
Antigen Society #4 Report (A2, AW68, AW69 and A28)....Pages 126-127
Antigen Society #5 Report (B5, B51, Bw52, B18, B35, Bw53)....Pages 128-132
Antigen Society #6 Report (B21, B49, Bw50, BN21, B12, B44, B45)....Pages 133-147
Antigen Society #7 Report (B8, w59, B14, w64, w65)....Pages 148-150
Antigen Society #8 Report (Bw70, Bw71, Bw72)....Pages 151-153
Antigen Society #9 Report (Bw46 and the Subgroups of B15)....Pages 153-195
Antigen Society #10 Report (Bw57 and Bw58)....Pages 196-197
Antigen Society #11 Report (B37, B38, B39, and Bw67)....Pages 197-198
Antigen Society #12 Report (Bw54, Bw55, Bw56 and Bw42)....Pages 199-202
Antigen Society #13 Report (B7, B27, Bw47, Bw73)....Pages 202-203
Antigen Society #13 Report (B7, B27, Bw47, Bw73): Public Determinants of the HLA-B7 CREG Defined by Antibodies and T-Cell Clones....Pages 204-206
Antigen Society #13 Report (B7, B27, Bw47, Bw73): Six Subtypes of HLA-B7 Defined by Histotypes, Epitopes and 1D-IEF....Pages 207-208
Antigen Society #14 Report (B40 CREG, BW60, BW61, BW41, BW48, B13)....Pages 209-214
Antigen Society #15 Report (Bw4 and Bw6)....Pages 214-217
Antigen Society #16 Report (Cw1, Cw3, Cw9, Cwl0, Cw11)....Pages 217-222
Antigen Society #17 Report (Cw5 and Cw8)....Pages 222-222
Antigen Society #18 Report (Cw4 and Cw6)....Pages 222-235
Front Matter....Pages 91-91
Antigen Society #19 Report (Cw7)....Pages 236-236
Antigen Society #20 Report (Cw2)....Pages 237-238
Antigen Society #21 Report DR1, DRw10)....Pages 238-240
Antigen Society #22 Report (DR2)....Pages 240-242
Antigen Society #23 Report (DR4, DR9): Part 1: Analysis of Core and Antigen Society #23 Panel Data....Pages 242-245
Antigen Society #23 Report (DR4, DR9): Part 2: Serologic Typing of B-Lymphoblastoid Cell Lines: DR4 and DR9....Pages 245-249
Antigen Society #24 Report (DRw11, DRwl2, DRw8)....Pages 249-251
Antigen Society #25 Report DRw6, DRw13, DRw14)....Pages 252-255
Antigen Society #26 Report (DR3, DR7, DQw2): Part 1....Pages 255-258
Antigen Society #26 Report (DR3, DR7, DQw2): Part 2: HLA-DR3....Pages 259-260
Antigen Society #26 Report (DR3, DR7, DQw2): Part 3: Serologic Definition of HLA-DR7....Pages 260-263
Antigen Society #26 Report (DR3, DR7, DQw2): Part 4: HLA-DQw2....Pages 263-266
Antigen Society #27 Report DQw1....Pages 266-268
Antigen Society #28 Report (DQw3)....Pages 269-273
Antigen Society #29 Report (DRw52)....Pages 273-275
Antigen Society #30 Report (DRw53)....Pages 275-277
Antigen Society #31 Report (Qa and TL-Like Antigens) Part 1: The Leiden “Class IV” or “Class I-like” Workshop....Pages 277-280
Antigen Society #31 Report Part 2: Antigen Society #31 Report....Pages 281-283
Antigen Society #31 Report Part 3: Leukemic Blasts Express New HLA Class I-Like Alloantigens....Pages 283-285
Antigen Society #32 Report (DP)....Pages 286-288
Front Matter....Pages 91-91
Antigen Society #33 Report (Public Epitopes)....Pages 288-289
Antigen Society #35 Report DQw4 [DQ “WA”])....Pages 290-291
Micro EIA and Monoclonal Antibodies....Pages 292-301
Cytotoxic Monoclonal Antibodies....Pages 301-305
Front Matter....Pages 307-307
Population Analysis of Class I HLA Antigens by One-Dimensional Isoelectric Focusing Gel Electrophoresis: Workshop Summary Report....Pages 309-331
A Standardized Method for Detection of HLA-A and HLA-B Alleles by One-Dimensional Isoelectric Focusing (IEF) Gel Electrophoresis....Pages 332-335
Detection of HLA-Cw3 Subtypes and a New HLA-C Antigen by One-Dimensional Isoelectric Focusing (1D-IEF)....Pages 336-337
Identification of HLA-C Locus Products by One-Dimensional Isoelectric Focusing (1D-IEF)....Pages 338-339
Serological Definition of HLA-A2 Variants....Pages 340-341
Variants of HLA-Aw68 Recognized by Isoelectric Focusing....Pages 341-343
Biochemical Variants of the Bw70 Antigen (Bw71, Bw72)....Pages 343-344
B “SNA”: HLA-B5 Split Associated with Bw6 Studied by Serology and 1D-IEF....Pages 344-346
B40 CREG Variants Detected by Serology, IEF, and CTL....Pages 346-347
Serology and IEF Analysis of New HLA-B Locus Gene Products B“POT,” B“RI,” B“ST40,” and B“LEV”....Pages 347-349
HLA Class I Typing by Computer Analysis of 1D-IEF Data....Pages 349-351
Segregation Analysis of HLA Class I Determinants by Biochemistry....Pages 352-352
Charge Variants of HLA-A and B Gene Products....Pages 353-355
Front Matter....Pages 357-357
T-Cell Recognition of HLA Class I Molecules....Pages 359-362
Front Matter....Pages 363-363
Structural Polymorphism of the HLA Class II α and β Chains: Summary of the 10th Workshop 2-D Gel Analysis....Pages 365-380
DR1: 2-D Gel Patterns....Pages 380-382
Front Matter....Pages 363-363
DR2 (DRw15, 16): 2-D Gel Patterns....Pages 383-385
DR3 (DRw17, DRw18): 2-D Gel Patterns....Pages 385-387
DR4: 2-D Gel Patterns....Pages 388-390
DR5 (DRw11, DRw12): 2-D Gel Patterns....Pages 390-392
DRw6 (DRw13, DRw14): 2-D Gel Patterns....Pages 393-395
DR7: 2-D Gel Patterns....Pages 396-397
DRw8: 2-D Gel Patterns....Pages 397-399
DR9: 2-D Gel Patterns....Pages 399-401
DRw52: 2-D Gel Patterns....Pages 401-404
DRw53: 2-D Gel Patterns....Pages 404-406
DQw1 (DQw5, DQw6): 2-D Gel Patterns....Pages 407-410
DQw2: 2-D Gel Patterns....Pages 410-412
DQw3 (DQw7, DQw8, DQw9): 2-D Gel Patterns....Pages 412-415
DQw4 (Wa): 2-D Gel Patterns....Pages 415-417
DPα Chains: 2-D Gel Patterns....Pages 417-420
Binding Patterns of the Monoclonal Antibodies in the 10th Workshop 2-D Gel Analysis of Class II Antigens....Pages 420-425
Standardized Equipment, Reagents, and Techniques Developed for the 10th Workshop 2-D Gel Study of HLA Class II Antigens....Pages 425-427
Computerized Analysis of 2-D Gel Patterns of the HLA Class II Antigens....Pages 427-437
Front Matter....Pages 439-441
Background, Goals, and Experimental Design....Pages 443-448
Collection and Processing of HLA Class II Cellular Data....Pages 449-457
Front Matter....Pages 439-441
Statistical Analysis of HLA Class II Cellular Data....Pages 458-487
Immunogenetic Analysis of HLA Class II Cellular Data....Pages 488-502
DR1: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 502-504
DR2: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 504-507
DR3: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 507-509
DR4 and DQw3: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 509-513
DR5: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 513-515
DRw6: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 516-518
DR7, DQw2, and DRw53: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 518-521
DRw8 and DQw4: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 522-524
DRw52: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 524-526
DQw1: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 527-528
DP: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 529-529
T-cell Clones Recognizing Shared or Cross-Reactive Epitopes: Antigen Report of the Cellular Studies of the Tenth International Histocompatibility Workshop....Pages 529-531
Functional Polymorphisms of HLA Class II Gene Products Detected by T-Lymphocyte Clones: Summary of the Tenth International Histocompatibility Workshop Cellular Studies....Pages 532-550
Front Matter....Pages 551-551
Standardized Southern Blot Workshop Technique....Pages 553-560
Organization and Design of the Southern Blot Component of the Tenth Histocompatibility Workshop....Pages 560-566
Southern Blot Experiments: Data Processing....Pages 567-572
Front Matter....Pages 573-573
RFLP Standardization Report for DR Beta/SstI....Pages 575-578
RFLP Standardization Report for DR Beta/PstI....Pages 579-581
Front Matter....Pages 573-573
RFLP Standardization Report for DR Beta/KpnI....Pages 581-583
RFLP Standardization Report for DR Beta/EcoRV....Pages 584-587
RFLP Standardization Report for DR Beta/TaqI....Pages 587-590
RFLP Standardization Report for DR Beta/BglII....Pages 591-593
RFLP Standardization Report for DR Beta/PvuII....Pages 593-595
RFLP Standardization Report for DR Beta/EcoRI....Pages 596-598
RFLP Standardization Report for DR Beta/HindIII....Pages 598-601
RFLP Standardization Report for DR Beta/MsPI....Pages 601-604
RFLP Standardization Report for DR Beta/BamHI....Pages 605-607
RFLP Standardization Report for: DR Beta/HincII....Pages 608-608
RFLP Standardization Report for DQ Beta/SstI....Pages 608-612
RFLP Standardization Report for DQ Beta/PstI....Pages 613-614
RFLP Standardization Report for DQ Beta/KpnI....Pages 615-616
RFLP Standardization Report for DQ Beta/EcoRV....Pages 617-619
RFLP Standardization Report for DQ Beta/TaqI....Pages 620-622
RFLP Standardization Report for DQ Beta/BglII....Pages 623-624
RFLP Standardization Report for DQ Beta/PvuII....Pages 625-626
RFLP Standardization Report for DQ Beta/EcoRI....Pages 627-629
RFLP Standardization Report for DQ Beta/HindIII....Pages 629-631
RFLP Standardization Report for DQ Beta/MspI....Pages 632-634
Front Matter....Pages 573-573
RFLP Standardization Report for DQ Beta/BamHI....Pages 634-636
RFLP Standardization Report for DQ Beta/HincII....Pages 637-641
RFLP Standardization Report for DP Beta/SstI....Pages 641-645
RFLP Standardization Report for DP Beta/PstI....Pages 646-647
RFLP Standardization Report for DP Beta/KpnI....Pages 648-649
RFLP Standardization Report for DP Beta/EcoRV....Pages 650-652
RFLP Standardization Report for DP Beta/TaqI....Pages 652-654
RFLP Standardization Report for DP Beta/BgIII....Pages 655-656
RFLP Standardization Report for DP Beta/PvuII....Pages 657-659
RFLP Standardization Report for DP Beta/EcoRI....Pages 660-662
RFLP Standardization Report for DP Beta/HindIII....Pages 662-664
RFLP Standardization Report for DP Beta/MspI....Pages 664-667
RFLP Standardization Report for DP Beta/BamHI....Pages 667-669
RFLP Standardization Report for: DP Beta/HincII....Pages 670-673
RFLP Standardization Report for DO Beta/SstI....Pages 674-676
RFLP Standardization Report for DO Beta/PstI....Pages 677-679
RFLP Standardization Report for DO Beta/KpnI....Pages 679-679
RFLP Standardization Report for DR Beta/EcoRV....Pages 680-682
RFLP Standardization Report for DO Beta/TaqI....Pages 682-683
RFLP Standardization Report for DO Beta/BglII....Pages 684-685
Front Matter....Pages 573-573
RFLP Standardization Report for DO Beta/PvuII....Pages 686-687
RFLP Standardization Report for DO Beta/EcoRI....Pages 688-689
RFLP Standardization Report for DO Beta/HindIII....Pages 690-690
RFLP Standardization Report for DO Beta/MspI....Pages 690-692
RFLP Standardization Report for DO Beta/BamHI....Pages 692-694
RFLP Standardization Report for Class I/SstI....Pages 695-696
RFLP Standardization Report for Class I/PstI....Pages 696-699
RFLP Standardization Report for Class I/KpnI....Pages 700-702
RFLP Standardization Report for Class I/EcoRV....Pages 702-705
RFLP Standardization Report for Class I/TaqI....Pages 706-708
RFLP Standardization Report for Class I/BgIII....Pages 708-710
RFLP Standardization Report for Class I/PvuII....Pages 710-714
RFLP Standardization Report for Class I/EcoRI....Pages 714-716
RFLP Standardization Report for Class I/HindIII....Pages 716-718
RFLP Standardization Report for Class I/MspI....Pages 719-720
RFLP Standardization Report for Class I/BamHI....Pages 721-722
RFLP Standardization Report for BF/SstI....Pages 723-723
RFLP Standardization Report for BF/PstI....Pages 724-724
RFLP Standardization Report for BF/KpnI....Pages 725-726
RFLP Standardization Report for BF/TaqI....Pages 726-727
Front Matter....Pages 573-573
RFLP Standardization Report for BF/Bg1II....Pages 728-728
RFLP Standardization Report for BF/PvuII....Pages 729-729
RFLP Standardization Report for BF/EcoRI....Pages 730-730
RFLP Standardization Report for BF/HindIII....Pages 731-731
RFLP Standardization Report for BF/MspI....Pages 732-733
RFLP Standardization Report for BF/BamHI....Pages 734-734
RFLP Standardization Report for BF/EcoRV....Pages 735-736
RFLP Standardization Report for C2/SstI....Pages 737-738
RFLP Standardization Report for C2/PstI....Pages 739-740
RFLP Standardization Report for C2/KpnI....Pages 741-742
RFLP Standardization Report for C2/EcoRV....Pages 743-744
RFLP Standardization Report for C2/TaqI....Pages 745-745
RFLP Standardization Report for C2/Bg1II....Pages 746-746
RFLP Standardization Report for C2/PvuII....Pages 747-748
RFLP Standardization Report for C2/EcoRI....Pages 749-750
RFLP Standardization Report for C2/HindIII....Pages 750-751
RFLP Standardization Report for C2/MspI....Pages 752-753
RFLP Standardization Report for C2/BamHI....Pages 754-754
RFLP Standardization Report for C4/SstI....Pages 755-756
RFLP Standardization Report for C4/PstI....Pages 757-757
Front Matter....Pages 573-573
RFLP Standardization Report for C4/KpnI....Pages 758-759
RFLP Standardization Report for C4/EcoRV....Pages 759-761
RFLP Standardization Report for C4/TaqI....Pages 761-762
RFLP Standardization Report for C4/PvuII....Pages 763-764
RFLP Standardization Report for C4/EcoRI....Pages 765-767
RFLP Standardization Report for C4/HindIII....Pages 767-768
RFLP Standardization Report for C4/MspI....Pages 769-770
RFLP Standardization Report for C4/BamHI....Pages 770-771
RFLP Standardization Report for 210H/SstI....Pages 772-772
RFLP Standardization Report for 210H/PstI....Pages 772-773
RFLP Standardization Report for 210H/KpnI....Pages 774-775
RFLP Standardization Report for 210H/EcoRV....Pages 775-777
RFLP Standardization Report for 210H/TaqI....Pages 778-779
RFLP Standardization Report for 210H/BgIII....Pages 779-780
RFLP Standardization Report for 210H/PvuII....Pages 781-781
RFLP Standardization Report for 210H/EcoRI....Pages 782-784
RFLP Standardization Report for 210H/HindIII....Pages 784-785
RFLP Standardization Report for 210H/MspI....Pages 786-787
RFLP Standardization Report for 210H/BamHI....Pages 788-789
RFLP Standardization Report for DR Alpha/SstI....Pages 789-789
Front Matter....Pages 573-573
RFLP Standardization Report for DR Alpha/PstI....Pages 790-790
RFLP Standardization Report for DR Alpha/KpnI....Pages 791-792
RFLP Standardization Report for DR Alpha/EcoRV....Pages 792-794
RFLP Standardization Report for DR Alpha/TaqI....Pages 795-796
RFLP Standardization Report for DR Alpha/BgIII....Pages 796-798
RFLP Standardization Report for DR Alpha/PvuII....Pages 799-801
RFLP Standardization Report for DR Alpha/EcoRI....Pages 801-803
RFLP Standardization Report for DR Alpha/HindIII....Pages 804-804
RFLP Standardization Report for DR Alpha/MspI....Pages 805-806
RFLP Standardization Report for DQ Alpha/SstI....Pages 807-808
RFLP Standardization Report for DQ Alpha/PstI....Pages 809-810
RFLP Standardization Report for DQ Alpha/KpnI....Pages 811-812
RFLP Standardization Report for DQ Alpha/EcoRV....Pages 812-814
RFLP Standardization Report for DQ Alpha/TaqI....Pages 815-816
RFLP Standardization Report for DQ Alpha/BgIII....Pages 817-818
RFLP Standardization Report for DQ Alpha/PvuII....Pages 819-821
RFLP Standardization Report for DQ Alpha/EcoRI....Pages 822-823
RFLP Standardization Report for DQ Alpha/HindIII....Pages 824-825
RFLP Standardization Report For DQ Alpha/MspI....Pages 826-827
RFLP Standardization Report for DQ Alpha/BamHI....Pages 828-828
Front Matter....Pages 573-573
RFLP Standardization Report for DP Alpha/SstI....Pages 829-829
RFLP Standardization Report for DP Alpha/PstI....Pages 829-831
RFLP Standardization Report for DP Alpha/KpnI....Pages 832-833
RFLP Standardization Report for DP Alpha/EcoRV....Pages 834-836
RFLP Standardization Report for DP Alpha/TaqI....Pages 836-837
RFLP Standardization Report for DP Alpha/BglII....Pages 838-839
RFLP Standardization Report for DP Alpha/PvuII....Pages 840-842
RFLP Standardization Report for DP Alpha/EcoRI....Pages 842-843
RFLP Standardization Report for DP Alpha/HindIII....Pages 844-845
RFLP Standardization Report for DP Alpha/MspI....Pages 846-847
RFLP Standardization Report for DP Alpha/BamHI....Pages 848-848
Front Matter....Pages 849-849
DNA-RFLP Analysis: HLA-DR Beta Workshop Report....Pages 851-860
RFLP Analysis of DQ-Beta Chain Gene: Workshop Report....Pages 860-867
Workshop Report for the HLA-DP Region....Pages 867-876
Final Workshop Report: HLA Class I RFLP....Pages 877-881
Workshop Report of RFLP in C4, C2, BF, and 21OH Genes....Pages 881-884
Specificity Analysis of DRA Fragments in Core Cell Lines....Pages 885-886
Specificity Analysis of DQA Fragments in Core Cell Lines....Pages 886-888
Unusual DR-DQ Haplotypes....Pages 888-890
Linkage Disequilibrium, Interlocus Association and Ancestral Haplotypes....Pages 891-892
Front Matter....Pages 849-849
The Genomic Structure of Ancestral Haplotypes Revealed by Pulsed Field Gel Electrophoresis (PFGE)....Pages 893-895
Myasthenia Gravis: Joint Report....Pages 895-899
Analysis of HLA Recombinant Families by Southern Blots....Pages 900-908
Southern Blot Analysis of Rheumatoid Arthritis....Pages 909-913
An RFLP Study of HLA-D Gene Polymorphism in Multiple Sclerosis....Pages 913-915
DP RFLP Studies of the 72 Core Cell Lines Selected for the Southern Blot Protocol....Pages 916-920
Analysis of Class II RFLP Patterns and T-Cell Clone Reactions....Pages 921-942
HLA Class II Typing by the Workshop Defined RFLP Specificities in Unrelated Individuals....Pages 943-952
RFLP: Sequence Interrelations at the DPA and DPB Loci....Pages 952-959
Restriction Fragment Length Polymorphism of HLA Genes: Summary of the Tenth International Workshop Southern Blot Analysis....Pages 959-1023
Front Matter....Pages 1025-1025
First Domain Sequence Diversity of DR and DQ Subregion Alleles....Pages 1027-1031
Front Matter....Pages 1033-1033
A Display of Positive Correlations Obtained by Serology, Southern Blot Analysis, T-Lymphocyte Clones, and Two-Dimensional Gel Analysis Tested on the Reference Panel of B-Lymphoblastoid Cell Lines for Factors of the HLA System....Pages 1035-1067
Back Matter....Pages 1069-1084