Human Monoclonal Antibodies: Methods and Protocols

Front Matter ....Pages i-xiv
 Human Monoclonal Antibodies: The Benefits of Humanization  (Herman Waldmann)....Pages 1-10
 Cancer Immunotherapy: The Dawn of Antibody Cocktails  (Ilaria Marrocco, Donatella Romaniello, Yosef Yarden)....Pages 11-51
 IgM Natural Autoantibodies in Physiology and the Treatment of Disease  (Mahboobeh Fereidan-Esfahani, Tarek Nayfeh, Arthur Warrington, Charles L. Howe, Moses Rodriguez)....Pages 53-81
 Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies  (Madhuri Bhandaru, Anand Rotte)....Pages 83-108
 An Efficient Method to Generate Monoclonal Antibodies from Human B Cells  (Jenna J. Guthmiller, Haley L. Dugan, Karlynn E. Neu, Linda Yu-Ling Lan, Patrick C. Wilson)....Pages 109-145
 Isolation of Antigen-Specific, Antibody-Secreting Cells Using a Chip-Based Immunospot Array  (Hiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, Atsushi Muraguchi)....Pages 147-162
 Purification of Human Monoclonal Antibodies and Their Fragments  (Nicole Ulmer, Sebastian Vogg, Thomas Müller-Späth, Massimo Morbidelli)....Pages 163-188
 One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood  (Anna-Jasmina Donaubauer, Paul F. Rühle, Ina Becker, Rainer Fietkau, Udo S. Gaipl, Benjamin Frey)....Pages 189-212
 Humanization and Simultaneous Optimization of Monoclonal Antibody  (Taichi Kuramochi, Tomoyuki Igawa, Hiroyuki Tsunoda, Kunihiro Hattori)....Pages 213-230
 Antibody Fragments Humanization: Beginning with the End in Mind  (Nicolas Aubrey, Philippe Billiald)....Pages 231-252
 Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice  (Susana Magadán Mompó, África González-Fernández)....Pages 253-291
 Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab Framework  (Jeremy D. King, John C. Williams)....Pages 293-298
 Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen Receptors  (Keichiro Mihara, Tetsumi Yoshida, Joyeeta Bhattacharyya)....Pages 299-306
 Rapid Chimerization of Antibodies  (Koji Hashimoto, Kohei Kurosawa, Hidetaka Seo, Kunihiro Ohta)....Pages 307-317
 Phage Display Technology for Human Monoclonal Antibodies  (Marco Dal Ferro, Serena Rizzo, Emanuela Rizzo, Francesca Marano, Immacolata Luisi, Olga Tarasiuk et al.)....Pages 319-338
 Recombinant Antibody Selections by Combining Phage and Yeast Display  (Fortunato Ferrara, Maria Felicia Soluri, Daniele Sblattero)....Pages 339-352
 Epitope Mapping via Phage Display from Single-Gene Libraries  (Viola Fühner, Philip Alexander Heine, Kilian Johannes Carl Zilkens, Doris Meier, Kristian Daniel Ralph Roth, Gustavo Marçal Schmidt Garcia Moreira et al.)....Pages 353-375
 A High-Throughput Magnetic Nanoparticle-Based Semi-Automated Antibody Phage Display Biopanning  (Angela Chiew Wen Ch’ng, Azimah Ahmad, Zoltán Konthur, Theam Soon Lim)....Pages 377-400
 A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin  (Ivan Babic, Santosh Kesari, Mark C. Glassy)....Pages 401-415
 A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors  (Patricia Blundell, Richard Pleass)....Pages 417-421
 A Cell-Based Reporter Assay Measuring the Activation of Fc Gamma Receptors Induced by Therapeutic Monoclonal Antibodies  (Michihiko Aoyama, Minoru Tada, Akiko Ishii-Watabe)....Pages 423-429
 “BIClonals”: Production of Bispecific Antibodies in IgG Format in Transiently Transfected Mammalian Cells  (Dana Litvak-Greenfeld, Lilach Vaks, Stav Dror, Limor Nahary, Itai Benhar)....Pages 431-454
 Production of Stabilized Antibody Fragments in the E. coli Bacterial Cytoplasm and in Transiently Transfected Mammalian Cells  (Racheli Birnboim-Perach, Yehudit Grinberg, Lilach Vaks, Limor Nahary, Itai Benhar)....Pages 455-480
Back Matter ....Pages 481-483