HPLC and CE Principles and Practice

Front Cover......Page 1
HPLC and CE: Principles and Practice......Page 4
Copyright Page......Page 5
Contents......Page 8
Preface......Page 14
1.1 Introduction......Page 16
1.2 Classification of Liquid Chromatographic Methods......Page 17
1.3 Basic Concepts of HPLC......Page 22
1.4 Summary of Major Concepts......Page 35
References......Page 36
2.2 Normal-Phase Chromatography......Page 39
2.3 Reversed-Phase Chromatography......Page 44
2.5 Ion-Exchange Chromatography......Page 53
2.6 Size-Exclusion Chromatography......Page 61
2.7 Affinity Chromatography......Page 65
2.8 Chiral Separations......Page 72
2.9 Summary of Major Concepts......Page 77
References......Page 82
3.2 Solvent Delivery Systems......Page 86
3.3 Solvent Degassing......Page 93
3.4 Isocratic versus Gradient Pumping Systems......Page 95
3.5 Sample Introduction......Page 98
3.6 Columns......Page 100
3.7 Detectors......Page 105
3.8 Sample Preparation......Page 124
3.9 Troubleshooting......Page 132
3.10 Summary of Major Concepts......Page 144
References......Page 145
4.2 Classification of Electrophoretic Modes......Page 149
4.3 Basic Concepts of Capillary Electrophoresis......Page 151
4.4 Summary of Major Concepts......Page 165
References......Page 167
5.1 Introduction......Page 169
5.2 Capillary Zone Electrophoresis......Page 170
5.3 Micellar Electrokinetic Capillary Chromatography......Page 176
5.4 Capillary Gel Electrophoresis......Page 180
5.5 Capillary Electrochromatography......Page 185
5.6 Chiral Capillary Electrophoresis......Page 187
5.7 Capillary Isoelectric Focusing......Page 189
5.8 Capillary Isotachophoresis......Page 193
5.9 Summary of Major Concepts......Page 197
References......Page 198
6.1 Introduction......Page 200
6.2 High-Voltage Power Supply......Page 201
6.3 Sample Injection......Page 202
6.4 Capillaries......Page 205
6.5 Detection......Page 208
6.7 Sample Preparation......Page 220
6.8 Troubleshooting......Page 221
6.9 Summary of Major Concepts......Page 225
References......Page 226
7.2 Identification of Peaks......Page 229
7.3 Quantitation......Page 237
7.4 Summary of Major Concepts......Page 254
References......Page 255
8.2 Classification of Columns......Page 257
8.3 Theoretical and Practical Considerations......Page 260
8.4 Applications of Miniaturized Liquid Chromatography......Page 269
8.5 Separations on a Chip......Page 274
8.6 Summary of Major Concepts......Page 285
References......Page 286
Index......Page 290