High-Throughput Protein Production and Purification: Methods and Protocols

Front Matter ....Pages i-xvi
Front Matter ....Pages 1-1
 Overview of High-Throughput Cloning Methods for the Post-genomic Era  (Claudia Ortega, Cecilia Abreu, Pablo Oppezzo, Agustín Correa)....Pages 3-32
 Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins  (Joanne E. Nettleship, Heather Rada, Raymond J. Owens)....Pages 33-49
 Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins  (Edward Kraft, Yvonne Franke, Katharine Heeringa, Stephanie Shriver, Inna Zilberleyb, Christine Kugel et al.)....Pages 51-68
 High-Throughput Protein Production in Yeast  (Francisco J. Fernández, Sara Gómez, M. Cristina Vega)....Pages 69-91
 A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells  (Sarah M. Rue, Paul W. Anderson, Michelle R. Gaylord, Jessica J. Miller, Scott M. Glaser, Scott A. Lesley)....Pages 93-142
 A High-Throughput Automated Protein Folding System  (Kenneth W. Walker, Philip An, Dwight Winters)....Pages 143-161
Front Matter ....Pages 163-163
 High-Throughput Production of Oxidized Animal Toxins in Escherichia coli  (Yoan Duhoo, Ana Filipa Sequeira, Natalie J. Saez, Jeremy Turchetto, Laurie Ramond, Fanny Peysson et al.)....Pages 165-190
 High-Throughput Purification of Protein Kinases from Escherichia coli and Insect Cells  (Sebastian Mathea, Eidarus Salah, Stefan Knapp)....Pages 191-202
 Parallelized Microscale Expression of Soluble scFv  (Giulio Russo, Viola Fühner, André Frenzel, Michael Hust, Stefan Dübel)....Pages 203-211
 High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression  (Duygu Sari-Ak, Shervin Bahrami, Magdalena J. Laska, Petra Drncova, Daniel J. Fitzgerald, Christiane Schaffitzel et al.)....Pages 213-226
 High-Throughput Protein Production of Membrane Proteins in Saccharomyces cerevisiae  (Jennifer M. Johnson, Franklin A. Hays)....Pages 227-259
 High-Throughput E. coli Cell-Free Expression: From PCR Product Design to Functional Validation of GPCR  (Sandra Cortès, Fatima-Ezzahra Hibti, Frydman Chiraz, Safia Ezzine)....Pages 261-279
 High-Throughput Site-Directed Mutagenesis  (Claire Strain-Damerell, Nicola A. Burgess-Brown)....Pages 281-296
Front Matter ....Pages 297-297
 Hot CoFi Blot: A High-Throughput Colony-Based Screen for Identifying More Thermally Stable Protein Variants  (Ignacio Asial, Pär Nordlund, Sue-Li Dahlroth)....Pages 299-320
 High-Throughput Isolation of Soluble Protein Domains Using a Bipartite Split-GFP Complementation System  (Amélie Massemin, Stéphanie Cabantous, Geoffrey S. Waldo, Jean-Denis Pedelacq)....Pages 321-333
 High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization  (Daniel Some, Vladimir Razinkov)....Pages 335-359
 High-Throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography  (Alex J. Vecchio, Robert M. Stroud)....Pages 361-388
 A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production  (Hui Xu, Thomas Clairfeuille, Christine C. Jao, Hoangdung Ho, Zachary Sweeney, Jian Payandeh et al.)....Pages 389-402
 Adaption of the Leishmania Cell-Free Expression System to High-Throughput Analysis of Protein Interactions  (Wayne A. Johnston, Shayli Varasteh Moradi, Kirill Alexandrov)....Pages 403-421
 High-Throughput Protein–Protein Interaction Assays Using Tripartite Split-GFP Complementation  (Jean-Denis Pedelacq, Geoffrey S. Waldo, Stéphanie Cabantous)....Pages 423-437
 High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay  (Yoan Duhoo, Virginie Girault, Jeremy Turchetto, Laurie Ramond, Fabien Durbesson, Patrick Fourquet et al.)....Pages 439-476
 High-Throughput Protein Analysis Using Negative Stain Electron Microscopy and 2D Classification  (Christopher P. Arthur, Claudio Ciferri)....Pages 477-485
 High-Throughput Protein Production Combined with High- Throughput SELEX Identifies an Extensive Atlas of Ciona robusta Transcription Factor DNA-Binding Specificities  (Kazuhiro R. Nitta, Renaud Vincentelli, Edwin Jacox, Agnès Cimino, Yukio Ohtsuka, Daniel Sobral et al.)....Pages 487-517
 High-Throughput Micro-Characterization of RNA–Protein Interactions  (Sara Gómez, Francisco J. Fernández, M. Cristina Vega)....Pages 519-531
Back Matter ....Pages 533-537