Handbook of Biological Confocal Microscopy

Front Matter....Pages i-xiii
Foundations of Confocal Scanned Imaging in Light Microscopy....Pages 1-14
Fundamental Limits in Confocal Microscopy....Pages 15-26
Quantitative Fluorescence Imaging with Laser Scanning Confocal Microscopy....Pages 27-39
The Pixelated Image....Pages 41-51
Laser Sources for Confocal Microscopy....Pages 53-67
Non-laser Illumination for Confocal Microscopy....Pages 69-76
Objective Lenses For Confocal Microscopy....Pages 77-86
Size and Shape of The Confocal Spot: Control and Relation to 3D Imaging and Image Processing....Pages 87-91
The Intermediate Optical System of Laser-scanning Confocal Microscopes....Pages 93-103
Intermediate Optics in Nipkow Disk Microscopes....Pages 105-111
The Role of the Pinhole in Confocal Imaging Systems....Pages 113-126
Photon Detectors for Confocal Microscopy....Pages 127-139
Manipulation, Display, and Analysis of Three-Dimensional Biological Images....Pages 141-150
Three-dimensional Imaging on Confocal and Wide-field Microscopes....Pages 151-161
Direct Recording of Stereoscopic Pairs Obtained From Disk-scanning Confocal Light Microscopes....Pages 163-168
Fluorophores for Confocal Microscopy: Photophysics and Photochemistry....Pages 169-178
Image Contrast in Confocal Light Microscopy....Pages 179-195
Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy....Pages 197-205
A Comparison of Various Optical Sectioning Methods: The Scanning Slit Confocal Microscope....Pages 207-214
Back Matter....Pages 215-232