Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics, 2nd Edition

Content: 
Series Editors
Page ii

Copyright
Page iv

Contributors
Pages xiii-xvii

Preface
Page xix
Paul M. Wassarman, Philippe M. Soriano

Contents of Previous Volumes
Pages xxi-xlviii

Chapter one - Lentivirus Transgenesis
Pages 3-15
Alexander Pfeifer, Tiongti Lim, Katrin Zimmermann

Chapter Two - Germline Modification Using Mouse Spermatogonial Stem Cells
Pages 17-36
Mito Kanatsu-Shinohara, Takashi Shinohara

Chapter Three - Embryonic In Vivo Electroporation in the Mouse
Pages 37-50
Tetsuichiro Saito

Chapter Four - Current Applications of Transposons in Mouse Genetics
Pages 53-70
Adam J. Dupuy

Chapter Five - Functional Genomics in the Mouse using the Sleeping Beauty Transposon System
Pages 71-89
Kyoji Horie, Chikara Kokubu, Junji Takeda

Chapter Six - The Use of DNA Transposons for Cancer Gene Discovery in Mice
Pages 91-106
George Vassiliou, Roland Rad, Allan Bradley

Chapter Seven - A Practical Summary of Site-Specific Recombination, Conditional Mutagenesis, and Tamoxifen Induction of CreERT2
Pages 109-123
Konstantinos Anastassiadis, Stefan Glaser, Andrea Kranz, Kaj Bernhardt, A. Francis Stewart

Chapter Eight - A Recombineering Pipeline to Make Conditional Targeting Constructs
Pages 125-144
Jun Fu, Madeleine Teucher, Konstantinos Anastassiadis, William Skarnes, A. Francis Stewart

Chapter Nine - Confirmation of Recombination Site Functionality in Gene Targeting Vectors using Recombinase-Expressing Bacteria
Pages 145-151
M. David Stewart, Richard R. Behringer

Chapter Ten - Genetic Fate Mapping Using Site-Specific Recombinases
Pages 153-181
Emilie LeguГ©, Alexandra L. Joyner

Chapter Eleven - Mapping Cell Fate and Function Using Recombinase-Based Intersectional Strategies
Pages 183-213
Susan M. Dymecki, Russell S. Ray, Jun C. Kim

Chapter Twelve - Genome-Wide Forward Genetic Screens in Mouse ES Cells
Pages 217-242
Meng Amy Li, Stephen J. Pettitt, Kosuke Yusa, Allan Bradley

Chapter Thirteen - Gene Trap Mutagenesis in the Mouse
Pages 243-269
Roland H. Friedel, Philippe Soriano

Chapter Fourteen - A Wider Context for Gene Trap Mutagenesis
Pages 271-295
Joshua M. Brickman, Anestis Tsakiridis, Christine To, William L. Stanford

Chapter Fifteen - Mouse Mutagenesis with the Chemical Supermutagen ENU
Pages 297-312
Frank J. Probst, Monica J. Justice

Chapter Sixteen - Phenotype-Driven Mouse ENU Mutagenesis Screens
Pages 313-327
Tamara Caspary

Chapter Seventeen - Using ENU Mutagenesis for Phenotype-Driven Analysis of the Mouse
Pages 329-348
Rolf W. Stottmann, David R. Beier

Chapter Eighteen - Exploration of Self-Renewal and Pluripotency in ES Cells Using RNAi
Pages 351-365
Christoph Schaniel, Dung-Fang Lee, Foster C. Gonsalves, Ramanuj DasGupta, Ihor R. Lemischka

Chapter Nineteen - Transgenic RNAi Applications in the Mouse
Pages 367-386
Jost Seibler, Frieder Schwenk

Chapter Twenty - Gene Knockdown in the Mouse Through RNAi
Pages 387-414
Aljoscha Kleinhammer, Wolfgang Wurst, Ralf KГјhn

Chapter Twenty-one - In Vivo Analysis of Gene Knockdown in Tetracycline-Inducible shRNA Mice
Pages 415-427
Christopher S. Raymond, Lei Zhu, Thomas F. Vogt, Myung K. Shin

Chapter Twenty-Two - The Power of Reversibility: Regulating Gene Activities via Tetracycline-Controlled Transcription
Pages 429-453
Kai Schönig, Hermann Bujard, Manfred Gossen

Chapter Twenty-Three - Gene Expression Profiling of Mouse Oocytes and Preimplantation Embryos
Pages 457-480
Francesca E. Duncan, Richard M. Schultz

Chapter Twenty-Four - Interrogating the Transcriptome of Oocytes and Preimplantation Embryos
Pages 481-510
Anne E. Peaston, Joel H. Graber, Barbara B. Knowles, Wilhelmine N. de Vries

Chapter Twenty-five - Gene Expression Profiling of Mouse Embryos with Microarrays
Pages 511-541
Alexei A. Sharov, Yulan Piao, Minoru S.H. Ko

Author Index
Pages 543-569

Subject Index
Pages 571-580