Guide to Electroporation and Electrofusion

Content: 
Front Matter, Page iii
In Memorium, Page iv
Copyright, Page iv
Preface, Pages ix-x
1 - Overview of Electroporation and Electrofusion, Pages 1-6, Donald C. Chang, James A. Saunders, Bruce M. Chassy, Arthur E. Sowers
2 - Structure and Dynamics of Electric Field-Induced Membrane Pores as Revealed by Rapid-Freezing Electron Microscopy, Pages 9-27, Donald C. Chang
3 - Events of Membrane Electroporation Visualized on a Time Scale from Microsecond to Seconds, Pages 29-46, Kazuhiko Kinosita Jr., Masahiro Hibino, Hiroyasu Itoh, Masaya Shigemori, Ken'ichi Hirano, Yutaka Kirino, Tsuyoshi Hayakawa
4 - Time Sequence of Molecular Events in Electroporation, Pages 47-61, Tian Y. Tsong
5 - Electropores in Lipid Bilayers and Cell Membranes, Pages 63-76, Leonid V. Chernomordik
6 - Biophysical Considerations of Membrane Electroporation, Pages 77-90, Eberhard Neumann, Andreas Sprafke, Elvira Boldt, Hendrik Wolf
7 - Progress toward a Theoretical Model for Electroporation Mechanism: Membrane Electrical Behavior and Molecular Transport, Pages 91-117, James C. Weaver, Alan Barnett
8 - Mechanisms of Electroporation and Electrofusion, Pages 119-138, Arthur E. Sowers
9 - Interfacial Membrane Alteration Associated with Electropermeabilization and Electrofusion, Pages 139-153, J. Teissié, M.-P. Rols
10 - Membrane Fusion Kinetics, Pages 155-166, Dimiter S. Dimitrov
11 - Effects of Intercellular Forces on Electrofusion, Pages 167-178, Sek Wen Hui, David A. Stenger
12 - Dynamics of Cytoskeletal Reorganization in CV-1 Cells during Electrofusion, Pages 179-198, Qiang Zheng, Donald C. Chang
13 - Gene Transfer into Adherent Cells Growing on Microbeads, Pages 201-208, Huntington Potter, Stefan W.F. Cooke
14 - Gene Targeting and Electroporation, Pages 209-225, Laura H. Reid, Oliver Smithies
15 - Pollen Electrotransformation for Gene Transfer in Plants, Pages 227-247, James A. Saunders, Benjamin F. Matthews, Sally L. Van Wert
16 - Electrofusion of Plant Protoplasts and the Production of Somatic Hybrids, Pages 249-264, George W. Bates
17 - Electrotransformation of Bacteria by Plasmid DNA, Pages 265-290, Jack T. Trevors, Bruce M. Chassy, William J. Dower, Hans P. Blaschek
18 - Creating Vast Peptide Expression Libraries: Electroporation as a Tool to Construct Plasmid Libraries of Greater than 109 Recombinants, Pages 291-301, William J. Dower, Steven E. Cwirla
19 - Electroporation and Electrofusion Using a Pulsed Radio-Frequency Electric Field, Pages 303-326, Donald C. Chang, John R. Hunt, Qiang Zheng, Pie-Qiang Gao
20 - Electroinsertion: An Electrical Method for Protein Implantation into Cell Membranes, Pages 327-346, Youssef Mouneimne, Pierre-Franç Tosi, Roula Barhoumi, Claude Nicolau
21 - Electroporation as a Tool to Study Enzyme Activities in Situ, Pages 347-361, R.R. Swezey, D. Epel
22 - Comparison of PEG-Induced and Electric Field-Mediated Cell Fusion in the Generation of Monoclonal Antibodies against a Variety of Soluble and Cellular Antigens, Pages 363-369, Uwe Karsten, Peter Stolley, Bertolt Seidel
23 - Production of Genetically Identical Embryos by Electrofusion, Pages 371-391, Lawrence Charles Smith
24 - Development of Cell–Tissue Electrofusion for Biological Applications, Pages 393-410, Richard Heller, Richard Gilbert
25 - Novel Applications of Electroporation, Pages 411-425, Sean R. Gallagher, Donald C. Chang
26 - Design of Protocols for Electroporation and Electrofusion: Selection of Electrical Parameters, Pages 429-455, Donald C. Chang
27 - Protocols for Using Electroporation to Stably or Transiently Transfect Mammalian Cells, Pages 457-463, Huntington Potter
28 - Optimization of Electroporation Using Reporter Genes, Pages 465-470, Grant R. MacGregor
29 - Genetic Manipulation of Plant Cells by Means of Electroporation and Electrofusion, Pages 471-483, James A. Saunders, George W. Bates
30 - Protocols for the Transformation of Bacteria by Electroporation, Pages 485-499, William J. Dower, Bruce M. Chassy, Jack T. Trevors, Hans P. Blaschek
31 - Protocol for High-Efficiency Yeast Transformation, Pages 501-505, Daniel M. Becker, Leonard Guarente
32 - Protocols of Electroporation and Electrofusion for Producing Human Hybridomas, Pages 507-522, Martin I. Mally, Michael E. McKnight, Mark C Glassy
33 - Human Hybridoma Formation by Hypo-Osmolar Electrofusion, Pages 523-533, S.M. Maseehur Rehman, Susan Perkins, Ulrich Zimmermann, Steven K.H. Foung
34 - Electrically Induced Fusion and Activation in Nuclear Transplant Embryos, Pages 535-551, James M. Robl, Philippe Collas, Rafael Fissore, John Dobrinsky
35 - Pulse Generators for Electrofusion and Electroporation, Pages 555-569, Bruce M. Chassy, James A. Saunders, Arthur E. Sowers
Index, Pages 571-581