Green Fluorescent Proteins

Content: 
Series Editors
Page ii

Edited by
Page iii

Copyright page
Page iv

Contributors
Pages xi-xiii

Preface
Pages xv-xvi

Chapter 1: Biophysics of the Green Fluorescent Protein Original Research Article
Pages 1-18
F.G. Prendergast

Chapter 2: Understanding Structure—Function Relationships in the Aequorea victoria Green Fluorescent Protein Original Research Article
Pages 19-30
Andrew B. Cubitt, Leslie A. Woollenweber, Roger Heim

Chapter 3: Quantitative Imaging of the Green Fluorescent Protein (GFP) Original Research Article
Pages 31-48
David W. Piston, George H. Patterson, Susan M. Knobel

Chapter 4: Single-Molecule Fluorescence Detection of Green Fluorescence Protein and Application to Single-Protein Dynamics Original Research Article
Pages 49-73
Daniel W. Pierce, Ronald D. Vale

Chapter 5: Targeting GFP to Organelles Original Research Article
Pages 75-85
Francesca De Giorgi, Zimran Ahmed, Carlo Bastianutto, Marisa Brini, Laurence Sophie Jouaville, Robert Marsault, Marta Murgia, Paolo Pinton, Tullio Pozzan, Rosario Rizzuto

Chapter 6: Cytoskeletal Dynamics in Yeast Original Research Article
Pages 87-105
Janet L. Carminati, Tim Stearns

Chapter 7: Analysis of Nuclear Transport in Vivo Original Research Article
Pages 107-122
Paul Ferrigno, Pamela A. Silver

Chapter 8: GFP Fusion Proteins as Probes for Cytology in Fission Yeast Original Research Article
Pages 123-138
Kenneth E. Sawin

Chapter 9: GFP Variants for Multispectral Imaging of Living Cells Original Research Article
Pages 139-151
James Haseloff

Chapter 10: GFP Fusions to a Microtubule Motor Protein to Visualize Meiotic and Mitotic Spindle Dynamics in Drosophila Original Research Article
Pages 153-163
Sharyn A. Endow

Chapter 11: GFP as a Cell and Developmental Marker in the Drosophila Nervous System Original Research Article
Pages 165-181
Andrea Brand

Chapter 12: Using Time–Lapse Confocal Microscopy for Analysis of Centromere Dynamics in Human Cells Original Research Article
Pages 183-202
Kevin F. Sullivan, Richard D. Shelby

Chapter 13: Visualization of Large-Scale Chromatin Structure and Dynamics Using the lac Operator/lac Repressor Reporter System Original Research Article
Pages 203-222
Andrew S. Belmont, Gang Li, Gail Sudlow, Carmen Robinett

Chapter 14: Centrosome Dynamics in Living Cells Original Research Article
Pages 223-238
Aaron Young, Richard Tuft, Walter Carrington, Stephen J. Doxsey

Chapter 15: Transfections of Primary Muscle Cell Cultures with Plasmids Coding for GFP Linked to Full-Length and Truncated Muscle Proteins Original Research Article
Pages 239-260
Guissou A. Dabiri, Kenan K. Turnacioglu, Joseph C. Ayoob, Jean M. Sanger, Joseph W. Sanger

Chapter 16: Monitoring the Dynamics and Mobility of Membrane Proteins Tagged with Green Fluorescent Protein Original Research Article
Pages 261-281
J. Lippincott-Schwartz, J.F. Presley, K.J.M. Zaal, K. Hirschberg, C.D. Miller, J. Ellenberg

Chapter 17: Synchronous Real-Time Reporting of Multiple Cellular Events Original Research Article
Pages 283-291
Jeffrey D. Plautz, Steve A. Kay

Chapter 18: Visualizing Protein Interactions in Living Cells Using Digitized GFP Imaging and FRET Microscopy Original Research Article
Pages 293-314
Ammasi Periasamy, Richard N. Day

Chapter 19: Flow Cytometric Analysis and FACS Sorting of Cells Based on GFP Accumulation Original Research Article
Pages 315-341
David W. Galbraith, Michael T. Anderson, Leonard A. Herzenberg

Chapter 20: GFP Biofluorescence: Imaging Gene Expression and Protein Dynamics in Living Cells: Design Considerations for a Fluorescence Imaging Laboratory Original Research Article
Pages 343-367
Paul C. Goodwin

Index
Pages 369-379

Volumes in Series
Pages 381-386